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Literature DB >> 32124180

A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs.

Weiye Chen¹, Dongming Zhao¹, Xijun He², Renqiang Liu¹, Zilong Wang¹, Xianfeng Zhang², Fang Li¹, Dan Shan¹, Hefeng Chen¹, Jiwen Zhang¹, Lulu Wang¹, Zhiyuan Wen¹, Xijun Wang¹, Yuntao Guan², Jinxiong Liu¹, Zhigao Bu^3,4.

Abstract

African swine fever (ASF) is a devastating infectious disease in swine that is severely threatening the global pig industry. An efficacious vaccine is urgently required. Here, we used the Chinese ASFV HLJ/18 as a backbone and generated a series of gene-deleted viruses. The virulence, immunogenicity, safety, and protective efficacy evaluation in specific-pathogen-free pigs, commercial pigs, and pregnant sows indicated that one virus, namely HLJ/18-7GD, which has seven genes deleted, is fully attenuated in pigs, cannot convert to the virulent strain, and provides complete protection of pigs against lethal ASFV challenge. Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV, and as such is expected to play an important role in controlling the spread of ASFV.

Entities: Disease Gene Species

Keywords: African swine fever; pig; protective efficacy; safety; vaccine

Year: 2020 PMID： 32124180 PMCID： PMC7223596 DOI： 10.1007/s11427-020-1657-9

Source DB: PubMed Journal: Sci China Life Sci ISSN： 1674-7305 Impact factor: 6.038

Supplementary materials

32 in total

1. African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus.

Authors: Vivian O'Donnell; Lauren G Holinka; Douglas P Gladue; Brenton Sanford; Peter W Krug; Xiqiang Lu; Jonathan Arzt; Bo Reese; Consuelo Carrillo; Guillermo R Risatti; Manuel V Borca
Journal: J Virol Date: 2015-03-25 Impact factor: 5.103

2. Genetic characterization of African swine fever virus in Cameroon, 2010-2018.

Authors: Abel Wade; Jenna Elizabeth Achenbach; Carmina Gallardo; Tirumala Bharani K Settypalli; Abdoulkadiri Souley; Gaston Djonwe; Angelika Loitsch; Gwenaelle Dauphin; Jean Justin Essia Ngang; Onana Boyomo; Giovanni Cattoli; Adama Diallo; Charles Euloge Lamien
Journal: J Microbiol Date: 2019-03-30 Impact factor: 3.422

3. Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge.

Authors: Vivian O'Donnell; Guillermo R Risatti; Lauren G Holinka; Peter W Krug; Jolene Carlson; Lauro Velazquez-Salinas; Paul A Azzinaro; Douglas P Gladue; Manuel V Borca
Journal: J Virol Date: 2016-12-16 Impact factor: 5.103

4. Outbreak of African swine fever in South Korea, 2019.

Authors: Hyun-Joo Kim; Ki-Hyun Cho; Soo-Kyoung Lee; Da-Young Kim; Jin-Ju Nah; Hyun-Jeong Kim; Hyo-Jin Kim; Ji-Yong Hwang; Hyun-Joo Sohn; Jun-Gu Choi; Hae-Eun Kang; Yong-Joo Kim
Journal: Transbound Emerg Dis Date: 2020-01-28 Impact factor: 5.005

5. A nonessential African swine fever virus gene UK is a significant virulence determinant in domestic swine.

Authors: L Zsak; E Caler; Z Lu; G F Kutish; J G Neilan; D L Rock
Journal: J Virol Date: 1998-02 Impact factor: 5.103

6. Evaluation of an African swine fever (ASF) vaccine strategy incorporating priming with an alphavirus-expressed antigen followed by boosting with attenuated ASF virus.

Authors: Maria V Murgia; Mark Mogler; Andrea Certoma; Diane Green; Paul Monaghan; David T Williams; Raymond R R Rowland; Natasha N Gaudreault
Journal: Arch Virol Date: 2018-10-26 Impact factor: 2.574

7. Genetic characterization of African swine fever virus isolates from soft ticks at the wildlife/domestic interface in Mozambique and identification of a novel genotype.

Authors: C J Quembo; F Jori; W Vosloo; L Heath
Journal: Transbound Emerg Dis Date: 2017-09-17 Impact factor: 5.005

8. Outbreak of African Swine Fever, Vietnam, 2019.

Authors: Van Phan Le; Dae Gwin Jeong; Sun-Woo Yoon; Hye-Min Kwon; Thi Bich Ngoc Trinh; Thi Lan Nguyen; Thi To Nga Bui; Jinsik Oh; Joon Bae Kim; Kwang Myun Cheong; Nguyen Van Tuyen; Eunhye Bae; Thi Thu Hang Vu; Minjoo Yeom; Woonsung Na; Daesub Song
Journal: Emerg Infect Dis Date: 2019-07-17 Impact factor: 6.883

9. DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain.

Authors: Sun-Young Sunwoo; Daniel Pérez-Núñez; Igor Morozov; Elena G Sánchez; Natasha N Gaudreault; Jessie D Trujillo; Lina Mur; Marisa Nogal; Daniel Madden; Kinga Urbaniak; In Joong Kim; Wenjun Ma; Yolanda Revilla; Juergen A Richt
Journal: Vaccines (Basel) Date: 2019-01-28

10. Molecular Characterization of African Swine Fever Virus, China, 2018.

Authors: Shengqiang Ge; Jinming Li; Xiaoxu Fan; Fuxiao Liu; Lin Li; Qinghua Wang; Weijie Ren; Jingyue Bao; Chunju Liu; Hua Wang; Yutian Liu; Yongqiang Zhang; Tiangang Xu; Xiaodong Wu; Zhiliang Wang
Journal: Emerg Infect Dis Date: 2018-11-17 Impact factor: 6.883

75 in total

1. Deletion of the H108R Gene Reduces Virulence of the Pandemic Eurasia Strain of African Swine Fever Virus with Surviving Animals Being Protected against Virulent Challenge.

Authors: Elizabeth Vuono; Elizabeth Ramirez-Medina; Ediane Silva; Ayushi Rai; Sarah Pruitt; Nallely Espinoza; Alyssa Valladares; Lauro Velazquez-Salinas; Douglas P Gladue; Manuel V Borca
Journal: J Virol Date: 2022-07-06 Impact factor: 6.549

2. Evaluation of the Deletion of MGF110-5L-6L on Swine Virulence from the Pandemic Strain of African Swine Fever Virus and Use as a DIVA Marker in Vaccine Candidate ASFV-G-ΔI177L.

Authors: Elizabeth Ramirez-Medina; Elizabeth Vuono; Ediane Silva; Ayushi Rai; Alyssa Valladares; Sarah Pruitt; Nallely Espinoza; Lauro Velazquez-Salinas; Manuel V Borca; Douglas P Gladue
Journal: J Virol Date: 2022-07-11 Impact factor: 6.549

3. Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses.

Authors: Anusyah Rathakrishnan; Ana Luisa Reis; Katy Moffat; Linda K Dixon
Journal: Methods Mol Biol Date: 2022

4. Transcriptome profile of spleen tissues from locally-adapted Kenyan pigs (Sus scrofa) experimentally infected with three varying doses of a highly virulent African swine fever virus genotype IX isolate: Ken12/busia.1 (ken-1033).

Authors: Eunice Magoma Machuka; John Juma; Anne Wangari Thairu Muigai; Joshua Oluoch Amimo; Roger Pelle; Edward Okoth Abworo
Journal: BMC Genomics Date: 2022-07-19 Impact factor: 4.547

5. A Triplex PCR Method for Distinguishing the Wild-Type African Swine Fever Virus From the Deletion Strains by Detecting the Gene Insertion.

Authors: Zhao Huang; Zhiying Xu; Haoxuan Cao; Fanliang Zeng; Heng Wang; Lang Gong; Shengxun Zhang; Sen Cao; Guihong Zhang; Zezhong Zheng
Journal: Front Vet Sci Date: 2022-06-28

Review 6. Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update.

Authors: Dionigia Meloni; Giulia Franzoni; Annalisa Oggiano
Journal: Vaccines (Basel) Date: 2022-04-29

7. Development and Application of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Detection and Differentiation of EP402R-Deleted and Wild-Type African Swine Fever Virus.

Authors: Junhai Zhu; Weijun Jian; Yifan Huang; Qi Gao; Fei Gao; Huahan Chen; Guihong Zhang; Ming Liao; Wenbao Qi
Journal: Front Vet Sci Date: 2022-06-06