Literature DB >> 35575887

Isolation of Porcine Bone Marrow Cells and Generation of Recombinant African Swine Fever Viruses.

Anusyah Rathakrishnan1, Ana Luisa Reis2, Katy Moffat2, Linda K Dixon2.   

Abstract

Genetic manipulation of ASFV has been increasingly used not only for the development of live attenuated vaccines but also as an indispensable tool to further our understanding of the virus-host interactions. Here we present methods for isolation of porcine bone marrow cells and purification of recombinant ASFV using both chromogenic and fluorescent reporters. We also describe in detail a newly developed method to purify genetically modified ASFV using fluorescence-activated cell sorting (FACS).
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  ASFV; FACS; Porcine bone marrow cells; Recombinant virus; Single-cell isolation; Titration

Mesh：

Substances：

Year:  2022        PMID： 35575887     DOI： 10.1007/978-1-0716-2333-6_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  24 in total

1.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.

Authors:  W A MALMQUIST; D HAY
Journal:  Am J Vet Res       Date:  1960-01       Impact factor: 1.156

2.  Propagation, modification, and hemadsorption of African swine fever virus in cell cultures.

Authors:  W A MALMQUIST
Journal:  Am J Vet Res       Date:  1962-03       Impact factor: 1.156

Review 3.  Gaps in African swine fever: Analysis and priorities.

Authors:  M Arias; C Jurado; C Gallardo; J Fernández-Pinero; J M Sánchez-Vizcaíno
Journal:  Transbound Emerg Dis       Date:  2017-09-22       Impact factor: 5.005

4.  Simultaneous Deletion of the 9GL and UK Genes from the African Swine Fever Virus Georgia 2007 Isolate Offers Increased Safety and Protection against Homologous Challenge.

Authors:  Vivian O'Donnell; Guillermo R Risatti; Lauren G Holinka; Peter W Krug; Jolene Carlson; Lauro Velazquez-Salinas; Paul A Azzinaro; Douglas P Gladue; Manuel V Borca
Journal:  J Virol       Date:  2016-12-16       Impact factor: 5.103

5.  ICTV Virus Taxonomy Profile: Asfarviridae.

Authors:  Covadonga Alonso; Manuel Borca; Linda Dixon; Yolanda Revilla; Fernando Rodriguez; Jose M Escribano
Journal:  J Gen Virol       Date:  2018-03-22       Impact factor: 3.891

Review 6.  African swine fever - A review of current knowledge.

Authors:  Sandra Blome; Kati Franzke; Martin Beer
Journal:  Virus Res       Date:  2020-08-02       Impact factor: 3.303

Review 7.  Laboratory methods to study African swine fever virus.

Authors:  Patricia de León; María J Bustos; Angel L Carrascosa
Journal:  Virus Res       Date:  2012-10-03       Impact factor: 3.303

8.  African swine fever virus NL gene is not required for virus virulence.

Authors:  C L Afonso; L Zsak; C Carrillo; M V Borca; D L Rock
Journal:  J Gen Virol       Date:  1998-10       Impact factor: 3.891

9.  A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs.

Authors:  Weiye Chen; Dongming Zhao; Xijun He; Renqiang Liu; Zilong Wang; Xianfeng Zhang; Fang Li; Dan Shan; Hefeng Chen; Jiwen Zhang; Lulu Wang; Zhiyuan Wen; Xijun Wang; Yuntao Guan; Jinxiong Liu; Zhigao Bu
Journal:  Sci China Life Sci       Date:  2020-03-01       Impact factor: 6.038

10.  Deletion of the African Swine Fever Virus Gene DP148R Does Not Reduce Virus Replication in Culture but Reduces Virus Virulence in Pigs and Induces High Levels of Protection against Challenge.

Authors:  Ana L Reis; Lynnette C Goatley; Tamara Jabbar; Pedro J Sanchez-Cordon; Christopher L Netherton; David A G Chapman; Linda K Dixon
Journal:  J Virol       Date:  2017-11-30       Impact factor: 5.103
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