William F Pritchard1, David L Woods2, Juan A Esparza-Trujillo2, Matthew F Starost3, Michal Mauda-Havakuk2, Andrew S Mikhail2, Ivane Bakhutashvili2, Shelby Leonard2, Elizabeth C Jones2, Venkatesh Krishnasamy2, John W Karanian2, Bradford J Wood4. 1. Center for Interventional Oncology, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, 10 Center Dr., Room 3N320B, MSC 1182, Bethesda, MD 20892. Electronic address: william.pritchard@nih.gov. 2. Center for Interventional Oncology, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, 10 Center Dr., Room 3N320B, MSC 1182, Bethesda, MD 20892. 3. Division of Veterinary Resources, National Institutes of Health, 10 Center Dr., Room 3N320B, MSC 1182, Bethesda, MD 20892. 4. Center for Interventional Oncology, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, 10 Center Dr., Room 3N320B, MSC 1182, Bethesda, MD 20892; National Institute of Biomedical Imaging and Bioengineering and National Cancer Institute Center for Cancer Research, National Institutes of Health, 10 Center Dr., Room 3N320B, MSC 1182, Bethesda, MD 20892.
Abstract
PURPOSE: To assess the feasibility of transarterial chemoembolization with drug-eluting embolic (DEE) microspheres in a woodchuck model of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Nine woodchucks were studied: 4 normal animals and 5 animals infected with woodchuck hepatitis virus in which HCC had developed. Three animals with HCC underwent multidetector CT. A 3-F sheath was introduced into the femoral artery, and the hepatic arteries were selectively catheterized with 2.0-2.4-F microcatheters. Normal animals underwent diagnostic angiography and bland embolization. Animals with HCC underwent DEE transarterial chemoembolization with 70-150-μm radiopaque microspheres loaded with 37.5 mg doxorubicin per milliliter. Cone-beam CT and multidetector CT were performed. Following euthanasia, explanted livers underwent micro-CT, histopathologic examination, and fluorescence imaging of doxorubicin. RESULTS: The tumors were hypervascular and supplied by large-caliber tortuous vessels, with arteriovenous shunts present in 2 animals. There was heterogeneous enhancement on multidetector CT with areas of necrosis. Six tumors were identified. The most common location was the right medial lobe (n = 3). Mean tumor volume was 30.7 cm3 ± 12.3. DEE chemoembolization of tumors was achieved. Excluding the 2 animals with arteriovenous shunts, the mean volume of DEE microspheres injected was 0.49 mL ± 0.17. Fluorescence imaging showed diffusion of doxorubicin from the DEE microspheres into the tumor. CONCLUSIONS: Woodchuck HCC shares imaging appearances and biologic characteristics with human HCC. Selective catheterization and DEE chemoembolization may similarly be performed. Woodchucks may be used to model interventional therapies and possibly characterize radiologic-pathologic correlations. Published by Elsevier Inc.
PURPOSE: To assess the feasibility of transarterial chemoembolization with drug-eluting embolic (DEE) microspheres in a woodchuck model of hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Nine woodchucks were studied: 4 normal animals and 5 animals infected with woodchuck hepatitis virus in which HCC had developed. Three animals with HCC underwent multidetector CT. A 3-F sheath was introduced into the femoral artery, and the hepatic arteries were selectively catheterized with 2.0-2.4-F microcatheters. Normal animals underwent diagnostic angiography and bland embolization. Animals with HCC underwent DEE transarterial chemoembolization with 70-150-μm radiopaque microspheres loaded with 37.5 mg doxorubicin per milliliter. Cone-beam CT and multidetector CT were performed. Following euthanasia, explanted livers underwent micro-CT, histopathologic examination, and fluorescence imaging of doxorubicin. RESULTS: The tumors were hypervascular and supplied by large-caliber tortuous vessels, with arteriovenous shunts present in 2 animals. There was heterogeneous enhancement on multidetector CT with areas of necrosis. Six tumors were identified. The most common location was the right medial lobe (n = 3). Mean tumor volume was 30.7 cm3 ± 12.3. DEE chemoembolization of tumors was achieved. Excluding the 2 animals with arteriovenous shunts, the mean volume of DEE microspheres injected was 0.49 mL ± 0.17. Fluorescence imaging showed diffusion of doxorubicin from the DEE microspheres into the tumor. CONCLUSIONS:WoodchuckHCC shares imaging appearances and biologic characteristics with humanHCC. Selective catheterization and DEE chemoembolization may similarly be performed. Woodchucks may be used to model interventional therapies and possibly characterize radiologic-pathologic correlations. Published by Elsevier Inc.
Authors: Charles T Burke; John M Cullen; Andrei State; Sashi Gadi; Kathy Wilber; Michael Rosenthal; Anna Bulysheva; Anthony Pease; Mathew A Mauro; Henry Fuchs Journal: J Vasc Interv Radiol Date: 2011-09-29 Impact factor: 3.464
Authors: Luke R Wilkins; James R Stone; Jaime Mata; Alisha Hawrylack; Ewa Kubicka; David L Brautigan Journal: J Vasc Interv Radiol Date: 2017-10 Impact factor: 3.464
Authors: John G Thompson; William van der Sterren; Ivane Bakhutashvili; Imramsjah M van der Bom; Alessandro G Radaelli; John W Karanian; Juan Esparza-Trujillo; David L Woods; Andrew L Lewis; Bradford J Wood; William F Pritchard Journal: J Vasc Interv Radiol Date: 2018-03-02 Impact factor: 3.464
Authors: Michelle Dow; Rachel M Pyke; Brian Y Tsui; Ludmil B Alexandrov; Hayato Nakagawa; Koji Taniguchi; Ekihiro Seki; Olivier Harismendy; Shabnam Shalapour; Michael Karin; Hannah Carter; Joan Font-Burgada Journal: Proc Natl Acad Sci U S A Date: 2018-10-04 Impact factor: 11.205
Authors: Juan R Rodriguez-Madoz; Katherine H Liu; Jose I Quetglas; Marta Ruiz-Guillen; Itziar Otano; Julien Crettaz; Scott D Butler; Christine A Bellezza; Nathan L Dykes; Bud C Tennant; Jesus Prieto; Gloria González-Aseguinolaza; Cristian Smerdou; Stephan Menne Journal: J Virol Date: 2009-09-09 Impact factor: 5.103
Authors: Michal Mauda-Havakuk; Michael T Kassin; Andrew S Mikhail; Juan A Esparza-Trujillo; Ivane Bakhutashvili; David L Woods; Paul G Wakim; Matthew F Starost; John W Karanian; Bradford J Wood; William F Pritchard Journal: J Vasc Interv Radiol Date: 2021-11-17 Impact factor: 3.464
Authors: Quirina M B de Ruiter; Sheng Xu; Ming Li; William F Pritchard; Matthew F Starost; Armando Filie; Andrew S Mikhail; Michal Mauda-Havakuk; Juan A Esparza-Trujillo; Ivane Bakhutashvili; Pedram Heidari; Umar Mahmood; John W Karanian; Bradford J Wood Journal: Cardiovasc Intervent Radiol Date: 2021-05-21 Impact factor: 2.797
Authors: Andrew S Mikhail; Michal Mauda-Havakuk; Ayele H Negussie; Natalie Hong; Natalie M Hawken; Camella J Carlson; Joshua W Owen; Olga Franco-Mahecha; Paul G Wakim; Andrew L Lewis; William F Pritchard; John W Karanian; Bradford J Wood Journal: Int J Pharm Date: 2022-01-20 Impact factor: 6.510
Authors: Johnathan Zeng; Matthew F Starost; Michal Mauda-Havakuk; Andrew S Mikhail; Ari Partanen; Bradford J Wood; John W Karanian; William F Pritchard Journal: BMC Vet Res Date: 2020-11-23 Impact factor: 2.741
Authors: Danielle L Stolley; Anna Colleen Crouch; Aliçan Özkan; Erin H Seeley; Elizabeth M Whitley; Marissa Nichole Rylander; Erik N K Cressman Journal: Pharmaceutics Date: 2020-12-20 Impact factor: 6.321
Authors: Michal Mauda-Havakuk; Andrew S Mikhail; Matthew F Starost; Elizabeth C Jones; Baktiar Karim; David E Kleiner; Ari Partanen; Juan A Esparza-Trujillo; Ivane Bakhutashvili; Paul G Wakim; Michael T Kassin; Andrew L Lewis; John W Karanian; Bradford J Wood; William F Pritchard Journal: J Hepatocell Carcinoma Date: 2021-03-09
Authors: Anirudh Sharma; Erik Cressman; Anilchandra Attaluri; Dara L Kraitchman; Robert Ivkov Journal: Nanomaterials (Basel) Date: 2022-08-12 Impact factor: 5.719