Literature DB >> 321012

Isolation of amino-terminal fragment of lactose repressor necessary for DNA binding.

N Geisler, K Weber.   

Abstract

lac repressor can be dissected by trypsin into a homogenous tetrameric core (accounting for residues 60 to 347), carrying inducer binding activity, and the monomeric amino-terminal peptides ("headpieces") accounting for residues 1 to 59 and 1 to 51, respectively. This restriction of the action of trypsin on lac repressor is obtained in 1 M Tris-HCl (pH 7.5)-30% in glycerol at 25 degrees C since only the peptide bonds at lysine-59 and to a lesser extent after at arginine-51 are cleaved under these conditions. The headpieces can be purified by gel filtration. They have ordered secondary structure as revealed by circular dichroism studies. The monomeric headpieces show the relatively weak binding to nonoperator DNA but not the highly specific and strong binding to operator DNA typical for tetrameric lac repressor.

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Year:  1977        PMID: 321012     DOI: 10.1021/bi00624a020

Source DB:  PubMed          Journal:  Biochemistry        ISSN: 0006-2960            Impact factor:   3.162


  24 in total

1.  Plasticity of quaternary structure: twenty-two ways to form a LacI dimer.

Authors:  L Swint-Kruse; C R Elam; J W Lin; D R Wycuff; K Shive Matthews
Journal:  Protein Sci       Date:  2001-02       Impact factor: 6.725

2.  Fine-tuning function: correlation of hinge domain interactions with functional distinctions between LacI and PurR.

Authors:  Liskin Swint-Kruse; Christopher Larson; B Montgomery Pettitt; Kathleen Shive Matthews
Journal:  Protein Sci       Date:  2002-04       Impact factor: 6.725

3.  How different DNA sequences are recognized by a DNA-binding protein: effects of partial proteolysis.

Authors:  P C Supakar; X Y Zhang; S Githens; R Khan; K C Ehrlich; M Ehrlich
Journal:  Nucleic Acids Res       Date:  1989-11-11       Impact factor: 16.971

4.  An amino-terminal fragment of lac repressor binds specifically to lac operator.

Authors:  R T Ogata; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

Review 5.  Structural aspects of protein-DNA recognition.

Authors:  P S Freemont; A N Lane; M R Sanderson
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

6.  Comparison of simulated and experimentally determined dynamics for a variant of the Lacl DNA-binding domain, Nlac-P.

Authors:  L Swint-Kruse; K S Matthews; P E Smith; B M Pettitt
Journal:  Biophys J       Date:  1998-01       Impact factor: 4.033

7.  The lambda repressor contains two domains.

Authors:  C O Pabo; R T Sauer; J M Sturtevant; M Ptashne
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

8.  Role of the purine repressor hinge sequence in repressor function.

Authors:  K Y Choi; H Zalkin
Journal:  J Bacteriol       Date:  1994-03       Impact factor: 3.490

9.  Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

Authors:  M Fried; D M Crothers
Journal:  Nucleic Acids Res       Date:  1981-12-11       Impact factor: 16.971

10.  Perturbation of lac operator DNA modification by tryptic core protein from lac repressor.

Authors:  S P Manly; G N Bennett; K S Matthews
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

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