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Literature DB >> 287002

The lambda repressor contains two domains.

C O Pabo, R T Sauer, J M Sturtevant, M Ptashne.

Abstract

Papain digestion of the lambda phage repressor produces two fragments that are relatively resistant to further digestion. One includes the amino terminus (residues 1-92) and the other the carboxyl terminus (residues 132-236). Calorimetry shows that the amino-terminal fragment denatures near 50 degrees C and that the carboxyl-terminal fragment denatures near 70 degrees C. Intact repressor undergoes two denaturations, one near 50 degrees C and another near 70 degrees C. These and other data show that lambda repressor consists of two domains joined by a "connector" 40 amino acids long that is sensitive to proteases. The amino-terminal domain binds DNA, and the carboxyl-terminal domain oligomerizes.

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Year: 1979 PMID： 287002 PMCID： PMC383439 DOI： 10.1073/pnas.76.4.1608

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. Electron microscopic study of the repressor of bacteriophage lambda and its interaction with operator DNA.

Authors: C Brack; V Pirrotta
Journal: J Mol Biol Date: 1975-07-25 Impact factor: 5.469

2. An amino-terminal fragment of lac repressor binds specifically to lac operator.

Authors: R T Ogata; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1978-12 Impact factor: 11.205

3. Heat capacity and entropy changes in processes involving proteins.

Authors: J M Sturtevant
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

4. A thermodynamic approach to the problem of stabilization of globular protein structure: a calorimetric study.

Authors: P L Privalov; N N Khechinashvili
Journal: J Mol Biol Date: 1974-07-05 Impact factor: 5.469

5. Ionization of a nitrophenol-containing reporter group at the active site of papain.

Authors: S D Lewis; J A Shafer
Journal: Biochemistry Date: 1974-02-12 Impact factor: 3.162

6. Control and selectivity of lambda DNA transcription in lysogenic bacteria.

Authors: S Naono; F Gros
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

Review 8. Hydrolysis of proteins.

Authors: R L Hill
Journal: Adv Protein Chem Date: 1965

9. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages.

Authors: M Lieb
Journal: J Mol Biol Date: 1966-03 Impact factor: 5.469

10. A calorimetric study of thermally induced conformational transitions of ribonuclease A and certain of its derivatives.

Authors: T Y Tsong; R P Hearn; D P Wrathall; J M Sturtevant
Journal: Biochemistry Date: 1970-06-23 Impact factor: 3.162

73 in total

1. Genetic selection for dissociative inhibitors of designated protein-protein interactions.

Authors: S H Park; R T Raines
Journal: Nat Biotechnol Date: 2000-08 Impact factor: 54.908

2. The preferred substrate for RecA-mediated cleavage of bacteriophage 434 repressor is the DNA-bound dimer.

Authors: David R Pawlowski; Gerald B Koudelka
Journal: J Bacteriol Date: 2004-01 Impact factor: 3.490

3. pH-dependent autocleavage of lambda repressor occurs in the operator-bound form: characterization of lambda repressor autocleavage.

Authors: Kaushik Ghosh; Atasi Pal; Rajagopal Chattopadhyaya
Journal: Biochem J Date: 2004-04-15 Impact factor: 3.857

The lambda repressor contains two domains.

1. Electron microscopic study of the repressor of bacteriophage lambda and its interaction with operator DNA.

2. An amino-terminal fragment of lac repressor binds specifically to lac operator.

3. Heat capacity and entropy changes in processes involving proteins.

4. A thermodynamic approach to the problem of stabilization of globular protein structure: a calorimetric study.

5. Ionization of a nitrophenol-containing reporter group at the active site of papain.

6. Control and selectivity of lambda DNA transcription in lysogenic bacteria.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 8. Hydrolysis of proteins.

9. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages.

10. A calorimetric study of thermally induced conformational transitions of ribonuclease A and certain of its derivatives.

1. Genetic selection for dissociative inhibitors of designated protein-protein interactions.

2. The preferred substrate for RecA-mediated cleavage of bacteriophage 434 repressor is the DNA-bound dimer.

3. pH-dependent autocleavage of lambda repressor occurs in the operator-bound form: characterization of lambda repressor autocleavage.

4. Cooperativity in long-range gene regulation by the lambda CI repressor.

5. Bacteria-based in vivo peptide library screening using biopanning approach.

6. How different DNA sequences are recognized by a DNA-binding protein: effects of partial proteolysis.

7. Structural analysis of the carboxy terminus of bacteriophage lambda repressor determined by antipeptide antibodies.

8. The Leptospira interrogans lexA gene is not autoregulated.

9. The bacteriophage 434 repressor dimer preferentially undergoes autoproteolysis by an intramolecular mechanism.

10. Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.