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Literature DB >> 32101196

A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.

Santanu Maity¹, Xiaojian Wang, Subhamoy Das, Maomao He, Lee W Riley, Niren Murthy.

Abstract

The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin.

Entities: Chemical Disease Gene Species

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Year: 2020 PMID： 32101196 PMCID： PMC7666973 DOI： 10.1039/c9cc09498a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

20 in total

1. Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions.

Authors: André Galarneau; Martin Primeau; Louis-Eric Trudeau; Stephen W Michnick
Journal: Nat Biotechnol Date: 2002-06 Impact factor: 54.908

Review 2. Molecular analysis of beta-lactamase structure and function.

Authors: Fahd K Majiduddin; Isabel C Materon; Timothy G Palzkill
Journal: Int J Med Microbiol Date: 2002-07 Impact factor: 3.473

3. Cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase activity.

Authors: Bengang Xing; Ashot Khanamiryan; Jianghong Rao
Journal: J Am Chem Soc Date: 2005-03-30 Impact factor: 15.419

4. Novel fluorogenic substrates for imaging beta-lactamase gene expression.

Authors: Wenzhong Gao; Bengang Xing; Roger Y Tsien; Jianghong Rao
Journal: J Am Chem Soc Date: 2003-09-17 Impact factor: 15.419

Review 5. Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small-Molecule Chemiluminescence Probes.

Authors: Michal Roth-Konforti; Ori Green; Mario Hupfeld; Lars Fieseler; Nadine Heinrich; Julian Ihssen; Raffael Vorberg; Lukas Wick; Urs Spitz; Doron Shabat
Journal: Angew Chem Int Ed Engl Date: 2019-06-24 Impact factor: 15.336

6. Covalent protein labeling based on noncatalytic beta-lactamase and a designed FRET substrate.

Authors: Shin Mizukami; Shuji Watanabe; Yuichiro Hori; Kazuya Kikuchi
Journal: J Am Chem Soc Date: 2009-04-15 Impact factor: 15.419

7. Point-of-Care Detection of β-Lactamase in Milk with a Universal Fluorogenic Probe.

Authors: Yiping Chen; Yunlei Xianyu; Jing Wu; Wenfu Zheng; Jianghong Rao; Xingyu Jiang
Journal: Anal Chem Date: 2016-05-06 Impact factor: 6.986

8. A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta-galactosidase in lacZ-transfected cells.

Authors: V K Jain; I T Magrath
Journal: Anal Biochem Date: 1991-11-15 Impact factor: 3.365

A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.

1. Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions.

Review 2. Molecular analysis of beta-lactamase structure and function.

3. Cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase activity.

4. Novel fluorogenic substrates for imaging beta-lactamase gene expression.

Review 5. Ultrasensitive Detection of Salmonella and Listeria monocytogenes by Small-Molecule Chemiluminescence Probes.

6. Covalent protein labeling based on noncatalytic beta-lactamase and a designed FRET substrate.

7. Point-of-Care Detection of β-Lactamase in Milk with a Universal Fluorogenic Probe.

8. A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta-galactosidase in lacZ-transfected cells.

9. Rapid point-of-care detection of the tuberculosis pathogen using a BlaC-specific fluorogenic probe.

10. Chemiluminescent determination of esterases in monocytes.

Review 1. Chemiluminescent spiroadamantane-1,2-dioxetanes: Recent advances in molecular imaging and biomarker detection.

2. β-Lactamase triggered visual detection of bacteria using cephalosporin functionalized biomaterials.

3. Advances in activity-based diagnostics for infectious disease and microbiome health.