| Literature DB >> 3202841 |
R Barra1, M Chiong, E González, C Vásquez.
Abstract
A type II modification methylase (M BstVI) was partially purified from the thermophilic bacterium Bacillus stearothermophilus V. The methylase catalyses the transfer of methyl groups from S-adenosyl-L-methionine to unmodified double-stranded DNA. The product of methylation was identified by paper chromatography as N6-methyladenine. Since M BstVI protects DNA against cleavage by BstVI and XhoI restriction endonucleases, it follows that it methylates the adenine residue in the sequence 5'-C-T-C-G-A-G-3'.Entities:
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Year: 1988 PMID： 3202841 PMCID： PMC1135282
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857