Essia Sebai1,2, Raja Serairi3,4, Katerina Saratsi5, Amel Abidi6,7, Nadia Sendi4, Mohamed Aziz Darghouth7, Mark S Wilson8, Smaragda Sotiraki5, Hafidh Akkari7. 1. Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Manar II Tunis, Tunisia. essia.sebai@fst.utm.tn. 2. Laboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Tunisia. essia.sebai@fst.utm.tn. 3. National School of Health Sciences of Tunis, Tunis, Tunisia. 4. Laboratoire Des Plantes Aromatiques Et Medicinales, Centre de Biotechnologie de Borj-Cedria, B.P. 901, 2050, Hammam-Lif, Tunisie. 5. Veterinary Research Institute Hellenic Agricultural Organisation-Demeter (Former NAGREF, NAGREF Campus, 57001, Thermi, Thessalniki, Greece. 6. Faculty of Sciences of Tunis, University of Tunis El Manar, 2092, Manar II Tunis, Tunisia. 7. Laboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, 2020, Sidi Thabet, Tunisia. 8. Allergy and Anti-Helminth Immunity Laboratory, The Francis Crick Institute, London, UK.
Abstract
INTRODUCTION: During recent decades, the emergence of chemoresistance among synthetic anthelmintic drugs has increased the interest in screening novel natural anthelmintic compounds derived from plants. The current study is aimed to determine the chemical profile, anthelmintic and antioxidant properties of Mentha pulegium hydro-ethanolic extract. MATERIALS AND METHODS: Two tests were used to assess the in vitro anthelmintic activity of the hydro-ethanolic extract of M. pulegium against Haemonchus contortus; egg hatch assay (EHA) and adult worm motility (AWM) assay. M. pulegium extracts at the doses of 500, 1000, 2000 and 4000 mg/kg were evaluated in vivo in mice infected with Heligmosomoides polygyrus. The anthelmintic efficacy was monitored using faecal egg count reduction (FECR) and total worm count reduction (TWCR). The antioxidant activity of M. pulegium extract was evaluated by testing the total antioxidant capacity and the DPPH free radical-scavenging ability. RESULTS: Chromatographic characterization of M. pulegium composition using RP-HPLC revealed the presence of phenolic acids such as syringic acid, ferulic acid and the presence of flavonoid compounds, such as isorhamnetin-3-O-glucoside and kaempferol-3-O-rutinoside. We observed 91.58% inhibition in the EHA at 8 mg/mL after 48 h of incubation (IC50=1.82 mg/mL). In the AWM assay, M. pulegium extract achieved 65.2% inhibition at 8 mg/mL after 8 h. The highest dose (4000 mg/kg) showed a significant nematicidal effect 7 days post-treatment by inducing 60.39% FECR and 71.6% TWCR. We also report strong in vivo antioxidant capacity of the extract, as revealed by a significant increase of the enzymatic activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in mice infected with H. polygyrus. CONCLUSION: Together, the results in this paper suggest that M. pulegium possesses anthelmintic properties and could be a potential source of novel compounds for the control of helminth parasites as well as its associated oxidative damage.
INTRODUCTION: During recent decades, the emergence of chemoresistance among synthetic anthelmintic drugs has increased the interest in screening novel natural anthelmintic compounds derived from plants. The current study is aimed to determine the chemical profile, anthelmintic and antioxidant properties of Mentha pulegium hydro-ethanolic extract. MATERIALS AND METHODS: Two tests were used to assess the in vitro anthelmintic activity of the hydro-ethanolic extract of M. pulegium against Haemonchus contortus; egg hatch assay (EHA) and adult worm motility (AWM) assay. M. pulegium extracts at the doses of 500, 1000, 2000 and 4000 mg/kg were evaluated in vivo in mice infected with Heligmosomoides polygyrus. The anthelmintic efficacy was monitored using faecal egg count reduction (FECR) and total worm count reduction (TWCR). The antioxidant activity of M. pulegium extract was evaluated by testing the total antioxidant capacity and the DPPH free radical-scavenging ability. RESULTS: Chromatographic characterization of M. pulegium composition using RP-HPLC revealed the presence of phenolic acids such as syringic acid, ferulic acid and the presence of flavonoid compounds, such as isorhamnetin-3-O-glucoside and kaempferol-3-O-rutinoside. We observed 91.58% inhibition in the EHA at 8 mg/mL after 48 h of incubation (IC50=1.82 mg/mL). In the AWM assay, M. pulegium extract achieved 65.2% inhibition at 8 mg/mL after 8 h. The highest dose (4000 mg/kg) showed a significant nematicidal effect 7 days post-treatment by inducing 60.39% FECR and 71.6% TWCR. We also report strong in vivo antioxidant capacity of the extract, as revealed by a significant increase of the enzymatic activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes in mice infected with H. polygyrus. CONCLUSION: Together, the results in this paper suggest that M. pulegium possesses anthelmintic properties and could be a potential source of novel compounds for the control of helminth parasites as well as its associated oxidative damage.
Authors: Aisha M H Al-Rajhi; Husam Qanash; Mohammed S Almuhayawi; Soad K Al Jaouni; Marwah M Bakri; Magdah Ganash; Hanaa M Salama; Samy Selim; Tarek M Abdelghany Journal: Molecules Date: 2022-07-28 Impact factor: 4.927