Literature DB >> 7000319

Development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of Brucella suis-infected swine.

C O Thoen, M P Hopkins, A L Armbrust, R D Angus, D E Pietz.   

Abstract

An enzyme-linked immunosorbent assay was developed using a heat-killed Brucella suis antigen for detecting antibodies in the sera of swine from which B. suis was isolated. Optimal enzyme-linked immunosorbent assay reactions were obtained using heat-killed B. suis antigen at a concentration comparable to McFarland Standard No. 1. Statistically significant differences were observed in the enzyme-linked immunosorbent assay results of 40 animals from which B. suis was isolated and the results for 48 noninfected swine at serum dilutions of 1:25 and 1:50 (P < 0.0001). The enzyme-linked immunosorbent assay is a rapid reproducible test which can be readily automated that appears to have practical value for screening large numbers of breeding and slaughter swine for brucellosis.

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Year:  1980        PMID： 7000319      PMCID： PMC1320076     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN： 0008-4050


  10 in total

1.  Development and evaluation of an enzyme-labeled antibody test for the rapid detection of hog cholera antibodies.

Authors:  G C Saunders
Journal:  Am J Vet Res       Date:  1977-01       Impact factor: 1.156

2.  An enzyme-labeled antibody test for detecting antibodies in chickens infected with Mycobacterium avium serotype 2.

Authors:  C O Thoen; W G Eacret; E M Himes
Journal:  Avian Dis       Date:  1978 Jan-Mar       Impact factor: 1.577

3.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

4.  Enzyme-linked immunosorbent assay for detecting antibodies in swine infected with Mycobacterium avium.

Authors:  C O Thoen; A L Armbrust; M P Hopkins
Journal:  Am J Vet Res       Date:  1979-08       Impact factor: 1.156

5.  Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.

Authors:  G C Saunders; E H Clinard
Journal:  J Clin Microbiol       Date:  1976-06       Impact factor: 5.948

6.  Reliability of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Trichinella spiralis infections in conventionally raised pigs.

Authors:  E J Ruitenberg; P A Steerenberg; B J Brosi; J Buys
Journal:  J Immunol Methods       Date:  1976       Impact factor: 2.303

7.  Enzyme immunoassay for detecting Brucella antibodies in cow's milk.

Authors:  C O Thoen; D E Pietz; A L Armbrust; R Harrington
Journal:  J Clin Microbiol       Date:  1979-08       Impact factor: 5.948

8.  Evaluation of the enzyme-linked immunosorbent assay for detecting Brucella abortus antibodies.

Authors:  J W Byrd; F C Heck; R J Hidalgo
Journal:  Am J Vet Res       Date:  1979-06       Impact factor: 1.156

9.  Use of an enzyme immunoassay test for characterizing the A and M antigens of Brucella.

Authors:  C O Thoen; A L Armbrust; W G Eacret; R Harrington; G M Brown
Journal:  J Clin Microbiol       Date:  1979-04       Impact factor: 5.948

10.  Cell-mediated immune responses in swine from a herd infected with Brucella suis.

Authors:  J M Kaneene; R K Anderson; D W Johnson; R D Angus; C C Muscoplat; D E Pietz; L C Vanderwagon; E E Sloane
Journal:  Am J Vet Res       Date:  1978-10       Impact factor: 1.156
  10 in total
  2 in total

1.  Evaluation of primary binding assays for presumptive serodiagnosis of swine brucellosis in Argentina.

Authors:  P S Paulo; A M Vigliocco; R F Ramondino; D Marticorena; E Bissi; G Briones; C Gorchs; D Gall; K Nielsen
Journal:  Clin Diagn Lab Immunol       Date:  2000-09

2.  Applications of enzyme-linked immunosorbent assay in veterinary medicine: a bibliography.

Authors:  S Charan; O P Gautam
Journal:  Vet Res Commun       Date:  1984-11       Impact factor: 2.459
  2 in total

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