Mette Ji Riis-Vestergaard1,2, Christoffer Laustsen3, Christian Østergaard Mariager3, Rolf F Schulte4, Steen Bønløkke Pedersen5,6,7, Bjørn Richelsen5,6,7. 1. Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Palle Juul Jensens Boulevard 99, 8200, Aarhus N, Denmark. metteriis@clin.au.dk. 2. Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark. metteriis@clin.au.dk. 3. MR Research Center, Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark. 4. GE Healthcare, Munich, Germany. 5. Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Palle Juul Jensens Boulevard 99, 8200, Aarhus N, Denmark. 6. Institute of Clinical Medicine, Aarhus University, Aarhus, Denmark. 7. Steno Diabetes Center Aarhus, Aarhus, Denmark.
Abstract
BACKGROUND/ OBJECTIVES: Brown adipose tissue (BAT) has gained growing interest as a potential target for treatment of obesity. Currently, the most widely used technique/method for in vivo measurements of BAT activity in humans is 18FDG PET/CT. To supplement these investigations novel radiation-free methods are warranted. Deuterium metabolic imaging (DMI) is a novel modality that combines magnetic resonance spectroscopic (MRS) imaging with deuterium-labelled glucose (2H-glucose). This allows for spatio-temporal and metabolic imaging beyond glucose uptake. We aimed to evaluate if DMI could discriminate glucose metabolism in BAT of cold-acclimatised and thermoneutral rats. SUBJECTS/ METHODS: Male Sprague-Dawley rats were housed in a cold environment (9 °C, n = 10) or at thermoneutrality (30 °C, n = 11) for 1 week. For imaging rats were anaesthetized, received a 2H-glucose (1 M, 1.95 g/kg) bolus and DMI was acquired at baseline followed by 20 min time intervals up to 2 h. Furthermore, Dixon MRI was performed for anatomical determination of the interscapular BAT (iBAT) depot along with dynamic contrast enhanced (DCE) MRI to evaluate perfusion. RESULTS: 2H-glucose signal was higher in cold-acclimatised rats compared with thermoneutral rats (p ≤ 0.001) indicating an overall increase in glucose uptake and metabolism. This was in line with a lower fat/water threshold, higher perfusion and increased UCP1 mRNA expression in iBAT (ninefold increment) of cold-acclimatised rats compared with thermoneutral rats. CONCLUSIONS: We find that DMI can discriminate cold-acclimatised and thermoneutral BAT in rats. This is the first study to evaluate BAT activity by DMI, which may open up for the use of the non-radioactive DMI method for BAT measurements in humans.
BACKGROUND/ OBJECTIVES: Brown adipose tissue (BAT) has gained growing interest as a potential target for treatment of obesity. Currently, the most widely used technique/method for in vivo measurements of BAT activity in humans is 18FDG PET/CT. To supplement these investigations novel radiation-free methods are warranted. Deuterium metabolic imaging (DMI) is a novel modality that combines magnetic resonance spectroscopic (MRS) imaging with deuterium-labelled glucose (2H-glucose). This allows for spatio-temporal and metabolic imaging beyond glucose uptake. We aimed to evaluate if DMI could discriminate glucose metabolism in BAT of cold-acclimatised and thermoneutral rats. SUBJECTS/ METHODS: Male Sprague-Dawley rats were housed in a cold environment (9 °C, n = 10) or at thermoneutrality (30 °C, n = 11) for 1 week. For imaging rats were anaesthetized, received a 2H-glucose (1 M, 1.95 g/kg) bolus and DMI was acquired at baseline followed by 20 min time intervals up to 2 h. Furthermore, Dixon MRI was performed for anatomical determination of the interscapular BAT (iBAT) depot along with dynamic contrast enhanced (DCE) MRI to evaluate perfusion. RESULTS:2H-glucose signal was higher in cold-acclimatised rats compared with thermoneutral rats (p ≤ 0.001) indicating an overall increase in glucose uptake and metabolism. This was in line with a lower fat/water threshold, higher perfusion and increased UCP1 mRNA expression in iBAT (ninefold increment) of cold-acclimatised rats compared with thermoneutral rats. CONCLUSIONS: We find that DMI can discriminate cold-acclimatised and thermoneutral BAT in rats. This is the first study to evaluate BAT activity by DMI, which may open up for the use of the non-radioactive DMI method for BAT measurements in humans.
Authors: Denis P Blondin; Amani Daoud; Taryn Taylor; Hans C Tingelstad; Véronic Bézaire; Denis Richard; André C Carpentier; Albert W Taylor; Mary-Ellen Harper; Céline Aguer; François Haman Journal: J Physiol Date: 2017-02-05 Impact factor: 5.182
Authors: Wouter D van Marken Lichtenbelt; Joost W Vanhommerig; Nanda M Smulders; Jamie M A F L Drossaerts; Gerrit J Kemerink; Nicole D Bouvy; Patrick Schrauwen; G J Jaap Teule Journal: N Engl J Med Date: 2009-04-09 Impact factor: 91.245
Authors: Kirsi A Virtanen; Martin E Lidell; Janne Orava; Mikael Heglind; Rickard Westergren; Tarja Niemi; Markku Taittonen; Jukka Laine; Nina-Johanna Savisto; Sven Enerbäck; Pirjo Nuutila Journal: N Engl J Med Date: 2009-04-09 Impact factor: 91.245
Authors: Xia Ge; Kyu-Ho Song; John A Engelbach; Liya Yuan; Feng Gao; Sonika Dahiya; Keith M Rich; Joseph J H Ackerman; Joel R Garbow Journal: Front Oncol Date: 2022-05-30 Impact factor: 5.738
Authors: Rohit Mahar; Huadong Zeng; Anthony Giacalone; Mukundan Ragavan; Thomas H Mareci; Matthew E Merritt Journal: Magn Reson Med Date: 2021-02-12 Impact factor: 3.737
Authors: Ayhan Gursan; Martijn Froeling; Arjan D Hendriks; Dimitri Welting; Arno P M Kentgens; Dennis W J Klomp; Jeanine J Prompers Journal: Magn Reson Med Date: 2021-10-17 Impact factor: 3.737
Authors: Loreen Ruhm; Nikolai Avdievich; Theresia Ziegs; Armin M Nagel; Henk M De Feyter; Robin A de Graaf; Anke Henning Journal: Neuroimage Date: 2021-10-09 Impact factor: 6.556
Authors: Yudu Li; Yibo Zhao; Rong Guo; Tao Wang; Yi Zhang; Matthew Chrostek; Walter C Low; Xiao-Hong Zhu; Zhi-Pei Liang; Wei Chen Journal: IEEE Trans Med Imaging Date: 2021-11-30 Impact factor: 10.048
Authors: Cornelius von Morze; John A Engelbach; Tyler Blazey; James D Quirk; Galen D Reed; Joseph E Ippolito; Joel R Garbow Journal: Magn Reson Med Date: 2020-11-28 Impact factor: 4.668