GSK-3β-Targeting Fisetin Promotes Melanogenesis in B16F10 Melanoma Cells and Zebrafish Larvae through β-Catenin Activation.

Fisetin is found in many fruits and plants such as grapes and onions, and exerts anti-inflammatory, anti-proliferative, and anticancer activity. However, whether fisetin regulates melanogenesis has been rarely studied. Therefore, we evaluated the effects of fisetin on melanogenesis in B16F10 melanoma cell and zebrafish larvae. The current study revealed that fisetin slightly suppressed in vitro mushroom tyrosinase activity; however, molecular docking data showed that fisetin did not directly bind to mushroom tyrosinase. Unexpectedly, fisetin significantly increased intracellular and extracellular melanin production in B16F10 melanoma cells regardless of the presence or absence of α-melanocyte stimulating hormone (α-MSH). We also found that the expression of melanogenesis-related genes such as tyrosinase and microphthalmia-associated transcription factor (MITF), were highly increased 48 h after fisetin treatment. Pigmentation of zebrafish larvae by fisetin treatment also increased at the concentrations up to 200 µM and then slightly decreased at 400 µM, with no alteration in the heart rates. Molecular docking data also revealed that fisetin binds to glycogen synthase kinase-3β (GSK-3β). Therefore, we evaluated whether fisetin negatively regulated GSK-3β, which subsequently activates β-catenin, resulting in melanogenesis. As expected, fisetin increased the expression of β-catenin, which was subsequently translocated into the nucleus. In the functional assay, FH535, a Wnt/β-catenin inhibitor, significantly inhibited fisetin-mediated melanogenesis in zebrafish larvae. Our data suggested that fisetin inhibits GSK-3β, which activates β-catenin, resulting in melanogenesis through the revitalization of MITF and tyrosinase.

1. Introduction

Melanin is important for the prevention of damages that occurs as a result of exposure to ultraviolet (UV) light [1]. The synthesis and storage of melanin occur inside a specific organelle in the melanocytes, called the melanosome, which then transfers melanin into adjacent keratinocytes [2]. Two main types of melanin are synthesized inside the melanosomes, namely eumelanin (black to brown melanin) and pheomelanin (reddish or yellowish melanin) [3]. The synthesis of these two types of melanin is regulated by three structurally related enzymatic proteins in the downstream melanogenic process: tyrosinase, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (DCT) [4]. When keratinocytes are exposed to the UV light, they secrete α-melanocyte stimulating hormone (α-MSH), a peptide hormone, which then binds to the melanocortin 1 receptor (MC1R) on the melanocytes and activates adenylyl cyclase (AC) leading to an increase in cyclic adenosine monophosphate (cAMP) [5]. As a result of the increase in cAMP, protein kinase A (PKA) is increased and subsequently phosphorylates the transcription factor cAMP response element (CRE)-binding protein (CREB) at SER 133 [6]. The phosphorylation of CREB results in the initiation of a transcriptional cascade of melanogenic processes including the induction of microphthalmia-associated transcription factor (MITF) expression, which ultimately stimulates the expression of tyrosinase, TYRP-1, and DCT [7].

Glycogen synthase kinase-3 (GSK-3) is a multi-tasking serine/threonine kinase, which transfers a phosphate group to either the serine or threonine residues of its substrates [8]. It was initially described as a key molecule for the inhibition of glycogen synthase in glycogen metabolism. However, recent studies have confirmed that GSK-3 is also involved in regulating many critical biological processes, including inflammation [9], tumorigenesis [10], Alzheimer’s disease (AD) [11], and Parkinson’s disease (PD) [12], and its phosphorylation is required for the initiation, enhancement, or inhibition of the function of target substances. There are two structurally similar GSK-3 isoforms in mammals, GSK-3α and GSK-3β, but they are functionally different [13]. In particular, GSK-3β plays a vital role in targeting β-catenin for proteasomal degradation via ubiquitination. When Wnt receptor complexes are not bound to a ligand, tumor suppressors axin, adenomatous polyposis coli (APC), GSK-3β, casein kinase I (CK1), protein phosphatase 2A (PP2A), and the E3-ubiquitin ligase β-TrCP form the β-catenin-destruction complex to phosphorylate β-catenin at its N-terminal domain and thereby consign β-catenin to proteasomal degradation [14,15]. In the presence of the Wnt ligand, a receptor complex is formed from the transmembrane protein frizzled (FZ), low-density lipoprotein receptor-related protein 6 (LRP6), and the scaffolding protein Dishevelled (Dvl) [14,15]. The formation of the complex results in the phosphorylation of LRP6 and the recruitment of axin to the complex, and thereby leads to the inhibition of axin-mediated β-catenin phosphorylation, which ultimately results in the stabilization of β-catenin. The stabilization and accumulation of β-catenin trigger the translocation of β-catenin to the nucleus to form a complex with T-cell transcription factor/lymphoid enhancer factor (TCF/LEF) and thereby activates the expression of Wnt target genes [16,17]. Interestingly, the phosphorylation at SER 9 inactivates GSK-3β and subsequently releases β-catenin from the destruction complex [18,19]. Previously, Schepsky et al. found that β-catenin directly binds with MITF and positively stimulated MITF-specific target genes, such as tyrosinase, which resulted in melanogenesis [20].

Mouse B16F10 melanoma cells have been used as a sensitive and reliable model for melanin quantification assays [21]. However, mouse B16 melanoma cells and normal melanocytes are associated with different response to 12-tetraadecnoylphorbol 13-acetate (TPA). In B16 melanoma cells, TPA (at 160 nM) inhibited melanogenesis by inhibiting protein kinase C-mediated MITF [22]; on the other hand, in mouse normal melanocytes, TPA (at 48 nM) stimulated melanogenesis by activating MITF [23]. In addition, exposure of TPA (at 85 nM, but not at 170 nM) stimulated melanin synthesis in human normal melanocytes by promoting tyrosinase activity [24]. Above studies showed that different cell types of normal melanocyte or melanoma inconsistently regulate melanogenesis or anti-melanogenesis because of different responsibility to TPA; thus, all reports used different concentrations of TPA, which indicates that according to the concentration of TPA, melanogenesis or anti-melanogenesis is regulated accompanied by TPA-responsive signaling. Especially, TPA at below 85 nM identically stimulated melanogenesis regardless of cell types such as mouse B16F10 melanoma cells and human normal melanocytes, which indicates that B16F10 cells are a sensitive melanogenesis model. Along with B16F10 melanoma cells, zebrafish larvae have been used as an attractive in vivo model for melanogenesis because the zebrafish model directly displays melanin strips, which can be visualized by the naked eye [25,26,27]. Zebrafish also shared genetic similarity with mouse and human during pigment expression from the neural crest-derived stem cells to melanocyte progenitor by activating MITF, TYRP-1, DCT, and tyrosinase [28,29].

Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a dietary flavonoid found in various fruits and vegetables, including strawberry, grape, apple, onion, and cucumber, at a concentration of 2–160 µg/g wet food [30]. Many studies have attempted to identify its biological effects, including neuroprotective, anti-arthritic, and anti-allergic activities [31,32,33]. In particular, emerging data also indicate that fisetin possesses anti-cancer activity, in in vitro and in vivo studies, including lung [34], bladder [35], breast [36], prostate [37], colon [38], and pancreatic cancers [39]. However, it is still contradictory whether fisetin is a positive or negative regulator of melanogenesis. Takekoshi et al. found that some flavonoids such as fisetin promotes melanin contents and tyrosinase activity in human melanoma cells [40]. On the other hands, Shon et al. reported that fisetin inhibits α-MSH-mediated intracellular and extracellular melanin content in murine B16F10 melanoma cells [41]. Therefore, in the present study, using in vitro and in vivo approaches, we tried to pinpoint the precise effect of fisetin on molecular mechanism involved in melanogenesis.

In the present study, we found that fisetin positively regulated melanogenesis in both B16F10 cells and an in vivo zebrafish model through the activation of the β-catenin signaling pathway. According to the molecular docking data, fisetin binds to the non-ATP–competitive site of GSK-3β; this suggests that fisetin promotes melanogenesis through the inhibition of GSK-3β and the subsequent release of β-catenin.

2. Results

2.1. Fisetin is a Non-Specific Inhibitor of Mushroom Tyrosinase Activity in Vitro

Fisetin contains a typical flavonol backbone with three additional hydroxyl functional groups (Figure 1A), which enables its action as an anticancer agent against various types of cancers. As the melanogenic effect of fisetin has been poorly determined, we, therefore, performed the mushroom tyrosinase activity assay in vitro. Overall, fisetin very slightly increased the inhibition of tyrosinase activity and highest concentration of fisetin (200 µM) resulted in the highest inhibition of mushroom tyrosinase activity in vitro (39.9% ± 4.7%) (Figure 1B). From fisetin concentrations at over 25 µM, the inhibitory activity slightly increased (11.2% ± 1.3%, 17.7% ± 1.7%, 17.0% ± 2.4%, and 25.3% ± 1.8% at 25 µM, 50 µM, 75 µM, and 100 µM fisetin, respectively). Then, we performed molecular docking analysis to determine whether fisetin directly bound to recombinant mushroom tyrosinase (PDB ID: 5M6B). Accordingly, the docking analysis showed that fisetin did not directly bind to the tyrosinase (Figure 1C), which suggested that the slight inhibitory effect of fisetin on tyrosinase activity was non-specific and due to anti-oxidant activity. Therefore, we hypothesized that fisetin slightly blocks the oxidation of tyrosine induced by tyrosinase. Collectively, these results suggest that fisetin could slightly inhibit in vitro mushroom tyrosinase enzyme activity without direct binding to tyrosinase, and that tyrosinase was not a direct target molecule of fisetin in melanogenesis.

Figure 1

Fisetin non-specifically inhibits in vitro mushroom tyrosinase enzyme activity without directly binding of tyrosinase. (A) The chemical structure of fisetin. (B) The effect of fisetin on in vitro mushroom tyrosinase activity. Tyrosinase activity was determined by the oxidation of L-tyrosinase as a substrate. Phenylthiourea (PTU) (200 nM) was used as positive control. (C) The molecular docking of fisetin with recombinant mushroom tyrosinase (PDB ID: 5M6B). The results are the average of the three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001, **, p < 0.01, and *, p < 0.05).

2.2. High Concentrations of Fisetin Decrease Relative Viability of B16F10 Melanoma Cells

To optimize the concentrations of fisetin for cellular melanogenic activity, cell morphology and MTT activity were measured at every 24 h-interval for 96 h after the treatment of B16F10 melanoma cells with fisetin. The microscopic data showed that fisetin (≤25 µM) resulted in no changes in morphology; however, high concentrations of fisetin (≥50 µM) downregulated total cell numbers without shrunk and round shape of cells (Figure 2A). Consistent with cell morphological analysis, MTT data showed that high concentrations of fisetin (≥50 µM) gradually decreased relative cell viability of B16F10 melanoma cells (Figure 2B). Nevertheless, in flow cytometry data, no distinct dead cells were observed (Figure 2C), which indicates that fisetin-mediated decrease of cell viability is not due to cell death. The results indicate that high concentrations of fisetin results in a decreased number of cells, but is not cytotoxic. Therefore, fisetin at below 25 µM was used for the subsequent experiments.

Figure 2

High concentrations of fisetin decrease the viability of B16F10 melanoma cells. (A) B16F10 melanoma cells were treated with the indicated concentrations (0–200 µM) of fisetin for 96 h and images were regularly captured at 24-h interval (10× Magnification). (B) After that, the same samples were used to determine the cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. (C) In a parallel experiment, the population of dead cells was analyzed by flow cytometry. The results are the average of three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001 and *, p < 0.05). 0v represents 0.01% DMSO (vehicle control).

2.3. Fisetin Increases Intracellular and Extracellular Melanin Content of B16F10 Melanoma Cells

To quantify intracellular and extracellular melanin content, B16F10 melanoma cells were treated with fisetin (5 µM and 20 µM) in the presence or absence of α-MSH for 96 h. Intracellular melanin content was assessed using the cell pellet extract, and extracellular melanin content was measured by the absorbance of culture medium. Unexpectedly, as shown in Figure 3A,B, 5 µM fisetin resulted in a moderate increase in spontaneous intracellular (157.0% ± 24.8% at 72 h and 207.5% ± 8.9% at 96 h) and extracellular melanin content (316.9% ± 9.3% at 72 h and 353.4% ± 3.4% at 96 h), compared with the untreated control. Treatment with 20 µM fisetin significantly increased intracellular melanin content to 224.3% ± 19.0% at 72 h and 293.4% ± 6.3% at 96 h and extracellular melanin content to 450.7% ± 80.7% at 72 h and 426.5% ± 6.1% at 96 h. The fisetin-mediated increase of spontaneous melanin content was comparable to that induced by 500 ng/mL α-MSH, which indicates that fisetin promotes in vitro melanogenesis in B16F10 melanoma cells. We also examined the intracellular and extracellular melanin content in α-MSH-treated B16F10 melanoma cells after treatment with fisetin (5 µM and 20 µM) for 96 h. We observed that fisetin strongly increased the α-MSH-induced intracellular (Figure 3C) and extracellular (Figure 3D) melanin content in B16F10 melanoma cells in a time-dependent manner compared with those induced by α-MSH treatment alone. The maximum effect occurred at 96 h at both fisetin concentrations tested (344.5% ± 8.7% and 406.2% ± 6.8% for intracellular melanin content at 5 µM and 25 µM fisetin and 148.3% ± 4.4% and 172.3% ± 3.1% for extracellular melanin content at 5 µM and 25 µM fisetin, respectively), which was comparable with the α-MSH-induced values of 291.4% ± 5.2% for intracellular melanin content and 142.4% ± 5.9% for extracellular melanin content. These results suggest that fisetin increases melanogenesis in B16F10 melanoma cells in both α-MSH-stimulated and unstimulated conditions.

Figure 3

Fisetin increases intracellular and extracellular melanin production in B16F10 melanoma cells. (A,B) B16F10 melanoma cells were cultured at a density of 1 × 104 cells/mL in 6 well plate overnight. Then, fisetin (5 µM and 20 µM) was treated for 96 h, and the cell pellet and the culture media were collected at every 24 h. (A) The cell pellets were washed with ice-cold PBS and dissolved in 400 µL of 1 M NaOH containing 10% DMSO at 90 ℃ for 60 min. Then, the absorbance was measured at 405 nm. (B) The culture media was directly measured at 405 nm for extracellular melanin contents. α-melanocyte stimulating hormone (α-MSH) (500 ng/mL) and PTU (200 nM) were used as the positive and the negative controls, respectively. (C,D) In a parallel experiment, B16F10 melanoma cells were treated with fisetin (5 µM and 20 µM) or PTU 2 h after treatment with 500 ng/mL α-MSH, and intracellular (C) and extracellular (D) melanin contents were measured at every 24 h for 96 h. The results are the averages of three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001, **, p < 0.01, and *, p < 0.05).

2.4. Fisetin Upregulates MITF and Tyrosinase Expression

As MITF is a key enzyme in the melanogenic pathway through the activation of tyrosinase activity, we examined the mRNA and protein expression of MITF and tyrosinase. B16F10 melanoma cells were treated with 20 µM fisetin and the mRNA was extracted. Fisetin significantly enhanced the expression of MITF and tyrosinase at 48 h, which completely disappeared from 72 h (Figure 4A). RT-PCR data also showed that fisetin concentration-dependently upregulated the expression of MITF and tyrosinase at 48 h and the highest concentration of fisetin (40 µM) was comparable with that of α-MSH treatment (Figure 4B). In addition, we purified the proteins after fisetin treatment for 72 h and measured the protein expression of MITF and tyrosinase. Consistent with RT-PCR data, we observed that fisetin increased the expression of both proteins in a dose-dependent manner and the highest concentration of fisetin induced the expression comparable with those of α-MSH treatment (Figure 4C). Altogether, these results indicate that fisetin increases both the mRNA and protein of MITF and tyrosinase, leading to melanogenesis.

Figure 4

Fisetin increases the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase (TYR) in B16F10 melanoma cells. B16F10 melanoma cells were cultured at a density of 1 × 104 cells/mL in 6 well plate overnight and then treated with the indicated concentrations of fisetin. (A) Time-dependent MITF and TYRexpression was detected by RT-PCR under stimulation with 20 µM fisetin for 96 h. (B) MITF and TYR expression was assessed 48 h after treatment with different concentrations of fisetin (0–40 µM) and 500 ng/mL α-MSH was used as a positive control. GAPDH was used as the house keeping gene for normalizing MITF and TYR expression. (C) B16F10 melanoma cells were treated with the indicated concentrations of fisetin for 72 h and western blotting was performed to analyze the expression of MITF and TYRprotein. β-Actin was used as a house keeping protein. The results are the average of three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001, **, p < 0.01, and *, p < 0.05).

2.5. Fisetin Inhibits Melanogenesis in Zebrafish Larvae but Did not Affect Heart Rate

As fisetin upregulates melanogenesis in B16F10 melanoma cells, we wondered whether fisetin could also increase melanogenesis in zebrafish larvae. After 24 h postfertilization, the chorion of the larvae was manually removed and fisetin was treated for an additional 3 days. On day 4, the images and the heart rate of the larvae were measured to assess melanogenic effect and cardiotoxicity of fisetin. Consistent with data in B16F10 melanoma cells, we found that fisetin strongly increased melanogenesis in zebrafish larvae in a concentration-dependent manner, with a maximum effect (212.0% ± 41.9%) at 200 µM fisetin, compared with the untreated control (Figure 5A,B). In addition, the heart rate remained almost similar to the untreated condition (179.7 ± 3.7 beats/min) (Figure 5C). Then, we tested the melanogenic effect in the presence of α-MSH in zebrafish larvae. First, we treated PTU for 24 h to remove all pigments and then α-MSH was treated 2 h prior to the fisetin treatment (50–200 µM) for 72 h. We found that fisetin concentration-dependently upregulated α-MSH-induced melanogenesis (167.1% ± 9.7%, 189.3% ± 8.5%, and 235.1% ± 15.7% at 50, 100, and 200 µM fisetin, respectively), compared with that of α-MSH treatment alone (145.8% ± 3.2%) (Figure 5D,E), and no alteration was observed in the heart rate of the zebrafish larvae compared with the untreated conditions (Figure 5F). Therefore, these results suggest that fisetin itself upregulates melanogenesis in zebrafish larvae, without causing cardiotoxicity.

Figure 5

Fisetin increases melanogenesis in a zebrafish model. (A–C) Zebrafish larvae were manually dechorionated at 1 dpf and exposed to the indicated concentrations of fisetin for another 72 h. (A) Images of the zebrafish larvae were captured using Olympus microscopy (4×) and (B) the relative density was calculated using ImageJ software. (C) The heart rate of zebrafish larvae was measured to assess the cardiotoxicity of fisetin. (D–F) In a parallel experiment, 200 μM PTU was treated in zebrafish larvae for 24 h and then administrated with 1 µg/mL α-MSH 2 h before fisetin treatment for 72 h. (D) The images were captured and (E) the relative density was calculated using ImageJ software. (F) The heart rate of zebrafish was used to measure the cardiotoxicity of the combined treatment. The results are the average of three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001, **, p < 0.01, and *, p < 0.05). 0v represents 0.01% DMSO (vehicle control).

2.6. Fisetin Possibly Binds to GSK-3β

GSK-3β is a constitutively active serine/threonine protein kinase that is involved in the hormonal control of glucose homeostasis, Wnt/β-catenin signaling, transcription factor regulation, and microtubules [8]. To investigate the structural binding of fisetin to GSK-3β, we performed computational docking of GSK-3β-fisetin interactions. Based on the interacting docking score, binding of amino acids with hydrogen bonds, and hydrogen bond distances, four predicted molecular docking complex models are shown in Table 1. In docking pose 1, the major residue of GSK-3β that interacts with fisetin is GLY34, at a distance of 2.191 Å, with a −7.9 docking score. Docking pose 2 and 3 also proposed that fisetin docked to GSK-3β through VAL101, ARG107, and ASP166 at a distance of 2.373 Å, 2.346 Å, and 2.247 Å (docking score: −7.9) and ARG107 of a distance of 1.029 Å (docking score: −7.9) through hydrogen bonding. Significant hydrogen bonding was not found in docking pose 4, but the docking score was −7.7. As shown in Figure 6, fisetin docked into the pocket of GSK-3β in four major ways, which displayed close contact with hydrogen bonds in the most energetically favorable simulation. The ATP-binding site and the activation loop of GSK-3β lie between the two domains near the hinge. Our molecular docking models showed that fisetin binds to GSK-3β at a non-ATP-competitive binding site near to the hinge regions (Figure 6). To compare the GSK-3β binding sites of fisetin with clinically available drugs, we performed the docking simulation with tideglusib (NP-12), enzastaurin (LY317615), and LY2090314. According to the molecular docking data, tideglusib, which is designed for the treatment of AD [42] and progressive supranuclear palsy (PSP), bound irreversibly to the non-ATP-competitive GSK-3β site [43] (Figure 7). The GSK-3β binding site of fisetin was also much more similar to that of tideglusib, which indicated that fisetin also binds irreversibly to the non-ATP-competitive GSK-3β site. Enzastaurin, which was originally developed as a selective inhibitor for PKCβ [5], and indirectly reduced the phosphorylation of GSK-3β through binding to ATP-binding site (Figure 7). The GSK-3 inhibitor LY2090314, which is used for the treatment of cancer [44], selectively targets the ATP-binding site as a competitive inhibitor (Figure 7). These data indicate that fisetin binds to a different binding site of GSK-3β than those previously identified.

Table 1

Classification of results gained from the docking of fisetin into glycogen synthase kinase-3β (GSK-3β).

Receptor	Docking Pose	Docking Score	Binding A.A. *(H-Bond) **	H-Bond Distance (Å)
GSK-3β(1J1B)	1	−7.9	GLY34	2.191
	2	−7.9	VAL101	2.373
			ARG107	2.346
			ASP166	2.247
	3	−7.9	ARG107	1.029
	4	−7.7	N.F.	N.F.

* A.A.: amino acid; ** H-bond: hydrogen bond.

Figure 6

Four molecular docking models show the binding site of fisetin and GSK-3β (PDB ID: 1J1B).

Figure 7

The molecular docking comparison of clinical GSK-3β inhibitors and fisetin with GSK-3β (PDB ID: 1J1B). The binding site of fisetin GSK-3β was compared with the clinically available drugs, tideglusib (NP-12), enzastaurin (LY317615), and LY2090314.

2.7. Activation of β-Catenin Positively Stimulates Fisetin-Mediated Melanogenesis

As the molecular docking prediction showed that fisetin binds to GSK-3β, we hypothesized that it promotes the release of β-catenin from the GSK-3β complex and thereby prevents the proteasomal degradation of β-catenin, leading to an increase in melanogenesis. Therefore, we investigated whether fisetin blocked the degradation of β-catenin and subsequently enhanced its nuclear translocation. Western blotting analysis confirmed that fisetin upregulated β-catenin expression in the cytosol and increased its nuclear translocation (Figure 8A). To evaluate the functional effect of β-catenin on fisetin-mediated melanogenesis, the intracellular and extracellular melanin content was detected in the presence of the Wnt/β-catenin inhibitor, FH535. Both fisetin-induced intracellular and extracellular melanin content significantly decreased in the presence of FH535 (Figure 8B,C), which suggested that fisetin-mediated melanogenesis was promoted by the activation of β-catenin. Interestingly, FH535 not only decreased fisetin-induced melanogenesis, but also α-MSH-mediated melanogenesis, which suggested that β-catenin plays a pivotal role in both fisetin- and α-MSH-induced melanogenesis. To confirm these results, zebrafish larvae were treated with 10 µM FH535 for 2 h and then exposed to fisetin or α-MSH for a further 72 h (Figure 8D). Consistent with the anti-melanogenic effects of FH535 in B16F10 cells, FH535 significantly downregulated both fisetin- and α-MSH-induced melanogenesis in zebrafish larvae without any change of the heart rate (Figure 8E), which confirmed that Wnt/β-catenin is a key molecule in both fisetin- and α-MSH-induced melanogenesis without any toxicity. Collectively, these results showed that fisetin suppresses active GSK-3β, which blocks β-catenin degradation and leads to melanogenesis.

Figure 8

Fisetin-induced melanogenesis is associated with the Wnt/β-catenin signaling pathway. (A) B16F10 melanoma cells were treated with the indicated concentrations of fisetin for 48 h and the expression of β-catenin in the cytosol (top) and the nucleus (bottom) was measured by western blotting analysis. β-Actin and nucleolin were used as the house keeping proteins. (B,C) The β-catenin inhibitor (10 µM), FH535, was pretreated for 2 h in B16F10 melanoma cells and then treated with 20 µM fisetin or 500 ng/mL α-MSH. After incubation of 72 h, the intracellular (B) and extracellular (C) melanin contents were examined. (D,E) Zebrafish larvae were manually dechorionated at 24 h and then 200 μM PTU was treated in zebrafish larvae for 24 h. Fisetin (200 µM) or α-MSH (1 µg/mL) were treated 72 h after treatment with FH535 for 2 h. (D) The images were collected at day 5 (right) and the relative density was calculated using ImageJ software (left). (E) The heart rate of the zebrafish was used to measure the toxicity of the combined treatment. The results are the average of three independent experiments; the data are expressed as the mean ± SEM (***, p < 0.001, **, p < 0.01, and *, p < 0.05).

3. Discussion

Fisetin is a bioactive diphenylpropane flavone structure that contains three aromatic rings with four additional hydroxyl groups and one oxo group [45]. It is abundant in various types of plants, but the natural biosynthesis of fisetin has not yet been described [46]. Fisetin is a powerful chemopreventive and chemotherapeutic candidate in a variety of cancers and ischemia-induced brain damage through its anti-oxidant activity [45]. In addition, He et al. reported that orally administered fisetin could cross the blood-brain barrier and lead to the promotion of long-term synaptic potentiation in the hippocampus [47]. Although fisetin has been evaluated for the beneficial pharmacological effects in animal models relevant to human diseases, whether fisetin positively or negatively regulates melanogenesis has still been contradictory [40,41]. Previously, the regulation of melanin synthesis by oxidation state was evaluated, which subjected melanocytes to the release of melanin from melanosomes [48]. Tyrosinase is a key component in melanogenesis since it catalyzes two major reactions in the Raper-Mason pathway. In one reaction, L-tyrosine is converted into its corresponding dopaquinone by the cresolase activity of tyrosinase and then subsequently dopaquinone is non-enzymatically converted to dihydroxyphenylalanine (DOPA) accompanied by O2- Generation [49]. In the other reaction, dopaquinone is also produced from L-DOPA by the catecholase activity of tyrosinase [50]. Therefore, we hypothesized that a powerful antioxidant, fisetin, was a negative regulator of melanogenesis in vitro and in vivo. As expected, high concentrations of fisetin (≥100 μM) inhibited in vitro mushroom tyrosinase activity, approximately 25%; however, the tyrosinase inhibition activity was low at below 25 μM fisetin, approximately below 10%. In addition, our molecular docking data confirmed that no direct binding was found between mushroom tyrosinase and fisetin, which indicates that fisetin could indirectly, inhibits melanin formation process. This discrepancy might be due to the initiation of cresolase and catecholase activity of tyrosinase enzyme at different time points. As Fenoll et al. [51] mentioned, addition of 3-methyl-2-benzothiazolinone hydrazine (MBTH), a potent nucleophile which traps enzyme-generated o-quinones in order to render MBTH-quinone adduct will provide a reliable measuring tool for determining the cresolase and catecholase activity of tyrosinase from fisetin. However, unexpectedly, fisetin (≤20 μM) significantly promoted intracellular melanin content and its release in B16F10 cells through the upregulation of MITF and tyrosinase expression, and also enhanced melanogenesis in zebrafish larvae. In addition, our molecular docking data present the first indication that fisetin targets GSK-3β, which consequently prevents the degradation of β-catenin and leads to the stimulation of melanogenesis (Figure 9). Nevertheless, whether fisetin directly regulates melanogenesis through human tyrosinase is disputable because mouse and human tyrosinase amino acid sequences are approximately 80% homologous.

Figure 9

The possible mechanism of fisetin promoting melanogenesis. Fisetin possibly binds to GSK-3β and subsequently inhibits its activity, which releases β-catenin. Free β-catenin moves to the nucleus and binds in the specific promoter region of MITF, which transactivates MITF expression, resulting in stimulation of tyrosinase-mediated melanogenesis.

Skin pigmentation provides the most important photoprotective effect against UV radiation [1] and it is a crucial factor in the removal of pigment formation from the skin by the cosmetic industry. Therefore, numerous attempts investigated to understand the underlying molecular mechanism that governs pigment production and their transfer into the adjacent keratinocytes [4,7]. In particular, a rate-limiting enzyme of melanogenesis, tyrosinase, promotes the generation of O2- in its catalytic response, which produces DOPA and dopaquinone [49], and suggested that the balance between the pro-oxidant and antioxidant state determines melanogenesis by the regulation of tyrosinase activity. At present, many antioxidants exerted anti-melanogenic activity through the suppression of tyrosinase and its regulatory genes, such as MITF [52]. As expected, fisetin, a powerful antioxidant slightly inhibited in vitro mushroom tyrosinase activity in the current study; in contrast, fisetin significantly increased intracellular and extracellular melanin contents in B16F10 cells and melanogenesis in a zebrafish larva model. This showed that fisetin directly regulated the intracellular signaling pathways and resulted in the positive regulation of melanogenesis. Consistent with the present data, Takekoshi et al. previously found that some flavonoids with hydroxyl group of the phenol ring (at 4′ in b, Figure 1A) such as fisetin (20 μM) increased melanin content and tyrosinase activity in human melanoma cells [40]. On the other hand, Son et al. reported that high concentrations of fisetin at over 50 μM decreased intracellular and extracellular melanin content in murine B16F10 melanoma cells [41]. We believe that the discrepancy on the dual effect of fisetin is dependent on its concentration because our unpublished data showed that high concentration of fisetin decreased melanogenesis in B16F10 melanoma cells (at 40 μM) and zebrafish larvae (at 400 μM). In addition, Kumagai et al. revealed that fisetin at 10 μM had no influence on melanogenesis and fisetin with methyl group significantly increased melanogenesis, which they indicate that methyl group is a key regulator for melanogenesis [53]; however, the data are confident that methylfisetin at 10 μM more strongly upregulates melanogenesis than fisetin at 10 μM but fisetin at 20 μM significantly increases melanogenesis. Collectively, fisetin bilaterally regulates melanogenesis in a concentration-dependent manner. We need further studies to confirm concentration-dependent bilateral effect of fisetin on melanogenesis.

MC1R is a G protein-coupled receptor, which activates AC and then leads to an increase in cAMP in response to α-MSH, which results in the activation of PKA [5]. Finally, PKA increased tyrosinase expression through the activation of CREB-mediated MITF [7]. This signaling pathway is the most common and crucial signaling pathway in melanogenesis. In the current study, we postulated that fisetin stimulated melanogenesis in vitro and in vivo through the activation of the cAMP-dependent signaling pathway, because our data showed that fisetin markedly increased tyrosinase, resulting from MITF expression at transcriptional and translational levels, which suggests that fisetin does not pass through the cAMP-dependent pathway of melanogenesis. The other negative regulatory pathway is the extracellular signal-regulated kinase (ERK) signaling pathway, which induces the phosphorylation of ERK1/2 through the activation of the c-kit-Ras-Raf axis and consequently enhances proteasome-mediated MITF degradation, which resulted in the anti-melanogenic effect. We also found that ERK1/2 was not a direct target of fisetin in accordance with the molecular docking prediction (data not shown). In addition, MITF transcription is upregulated by activating the Wnt/β-catenin signaling pathway, through the inhibition of GSK-3β, which results in melanogenesis. The accumulation of β-catenin stimulates TCF/LEF, which consequently transactivates the MITF promoter, dependent on TCF/LEF DNA consensus elements [54]. Interestingly, the molecular docking analysis revealed that fisetin strongly binds to GSK-3β at the non-ATP-competitive binding site and thereby enabled the release of β-catenin from the destructive complex. We also found that fisetin significantly increased intracellular β-catenin expression in B16F10 cells and that the inhibition of β-catenin, suppressed fisetin-mediated melanogenesis in B16F10 melanoma cells and zebrafish larva, which showed that fisetin influences melanogenesis through the activation of β-catenin via the inhibition of GSK-3β.

GSK-3β is an ubiquitously expressed serine/threonine protein kinase that is involved in glycogen synthesis in response to insulin [55] and energy metabolism, neuronal cell development, and body pattern formation [56]. Consistent with our data, a previous study determined that GSK-3β negatively regulated melanogenesis through the induction of the proteasome-mediated degradation of β-catenin, which caused the suppression of tyrosinase expression [54]. However, a more recent study showed that GSK-3β is crucially implicated in AD and PD through the direct interaction with tau, β-amyloid, and α-synuclein [57], which suggested that GSK-3β inhibition has become an attractive target for therapeutic intervention against AD and PD. In addition, owing to the conserved nature of the ATP-binding sites of GSK-3β, the drugs that effectively target GSK-3β act at nanomolar concentrations and non-ATP-competitive GSK-3β inhibitors act at relatively high (micromolar) concentrations [58]. Recently, Nabavi et al. broadly examined the chemistry, sources, bioavailability, and clinical impact of fisetin, and reported that fisetin was the potent neuroprotective flavonoid against AD and PD [59]. Therefore, fisetin is important, not only for the induction of melanogenesis, but may also provide a good platform to cure neurodegenerative diseases, including AD and PD. Other than AD and PD, GSK-3β has emerged as an interesting therapeutic target in pathological mechanisms, including inflammatory diseases, cancers, cardiovascular diseases, diabetes, and bone disorders [8]. Previous studies of John et al. revealed that GSK-3β inhibition prevent melanoma cell migration by downregulating the expression of N-cadherin and focal adhesion kinase (FAK) phosphorylation [60] indicating the possibility of using fisetin as therapeutic agent for metastatic melanoma. On the other hand, fisetin can be used as a remedy for hypo-pigmentary disorders such as vitiligo with the minimum side effects. Therefore, further study is needed to evaluate the various functions of fisetin in a broad spectrum of diseases.

We investigated the fisetin-mediated promotion of melanogenesis in B16F10 cells and zebrafish larvae through binding to GSK-3β at a non-ATP-competitive binding site, and the subsequent release of β-catenin, which promotes MITF-mediated tyrosinase activation. Although fisetin caused an unexpected increase in melanogenesis, fisetin may be useful for the treatment of many different diseases such as vitiligo and the inhibitory effect on GSK-3β is also paramount important.

4. Materials and Methods

4.1. Reagents and Antibodies

Fisetin, mushroom tyrosinase, phenylthiourea (PTU), and α-MSH were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Fisetin was dissolved in dimethyl sulfoxide (DMSO) as a stock solution at 50 mM concentration and stored at −20 °C. DMEM medium, fetal bovine serum (FBS), and antibiotic mixture were purchased from WELGENE (Gyeongsan-si, Gyeongsangbuk-do, Korea). Antibodies against MITF, tyrosinase, β-catenin, β-actin, and nucleolin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Peroxidase-labeled anti-rabbit and anti-mouse immunoglobulins were obtained from KOMA Biotechnology (Seoul, Korea). All other chemicals were purchased from Sigma grades.

4.2. Cell Culture and Viability Assay

Mouse B16F10 melanoma cells were obtained from ATCC (Manassas, VA, USA). The cells were cultured at 37 °C in a 5% CO2 humidified incubator in DMEM supplemented with 10% heat-inactivated FBS and antibiotic mixture. The cells were treated with various concentrations of fisetin for 96 h and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was performed at regular 24-h interval. Briefly, B16F10 melanoma cells were seeded at a density of 1 × 104 cells/mL in 24 well plate overnight. The cells were then treated with various concentrations of fisetin (0–200 μM) for 96 h. At every 24 h, final concentration of 0.5 mg/mL MTT solution was added to each well and incubated for 1 h at 37 °C to measure the mitochondrial related metabolism. Following the media removal, DMSO was added to each well and gently shaken for 10 min at room temperature. Dissolved formazan was transfer into 96 well plate and absorbance was determined at 540 nm by a microplate spectrophotometer (Thermo Fisher Scientific; Waltham, MA, USA).

4.3. In Vitro Mushroom Tyrosinase Activity

In vitro mushroom tryrosinase activity was performed according to the previous protocol with a little modifications [61]. In a 96 well plate, 100 mM potassium phosphate buffer (pH 6.5), different concentrations of fisetin, 1.5 mM L-tyrosine, and 210 U/mL mushroom tyrosinase were mixed. Phenylthiourea (PTU) (100 nM) was used as a positive control. Then, the microplate was incubated at 37 °C for 40 min and the absorbance of the mixture was measured at 490 nm. The value of each measurement was represented as percentage changes from the untreated control (reaction mixture without fisetin). Fisetin-treated group diluted in buffer without tyrosinase was carried out to exclude color interference in the absorbance measurement. The inhibition percentage of tyrosinase activity was calculated using following equation; Inhibition rate (%) = 100 × {[(A − B) − (C − D)]}/(A − B), where A is the absorbance at 490 nm (Abs490) without testing substance (L-tyrosine + tyrosinase), B is the Abs490 both without testing substance and tyrosinase (L-tyrosine), C is the Abs490 with testing substance (L-tyrosine + tyrosinase + sample) and D is Abs490 with testing substance but without tyrosinase (L-tyrosine + sample).

4.4. Flow Cytometry Analysis

B16F10 melanoma cells were seeded at a density of 1 × 104 cells/mL in 24 well plate overnight and then treated with fisetin (0–200 μM) for 96 h. The cells were collected and stained with Muse® cell viability assay kit (Luminex Co., Austin, TX, USA) for 10 min. The population of dead cell (%) was analyzed by Guava®Muse® Cell Analyzer (Luminex Co.).

4.5. Intracellular and Extracellular Melain Content

The effect of fisetin on melanin content in B16F10 melanoma cells was investigated according to the previous protocol [62]. Briefly, B16F10 melanoma cells were cultured at a density of 1 × 105 cells/mL in 6 well plate overnight. Then, the indicated concentrations of fisetin were treated for 96 h, and the culture media and the cell pellet were collected at every 24 h. The culture media was directly measured at 405 nm for extracellular melanin content. For intracellular melanin content, the cell pellets were washed with ice-cold PBS and dissolved in 400 µL of 1 M NaOH containing 10% DMSO at 90 °C for 60 min. Then, the absorbance was measured at 405 nm.

4.6. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Total RNA was extracted using easy-BLUE™ total RNA extraction kit (iNtRON Biotechnology, Seongnam-si, Gyeonggi, Korea) according to the manufacturer’s instruction. One microgram RNA was reverse-transcribed using MMLV reverse transcriptase (Bioneer, Daejeon-si, Korea). The cDNA was amplified using EzWay Taq PCR ReadyMix (KOMA Biotechnology) with specific primers of MITF (forward 5′-CCC GTC TCT GGA AAC TTG ATC G-3′ and reverse 5′-CTG TAC TCT GAG CAG CAG GTC-3′), tyrosinase (forward 5′-GTC GTC ACC CTG AAA ATC CTA ACT-3′ and reverse 5′-CAT CGC ATA AAA CCT GAT GGC′), and GAPDH (forward 5′- AGG TCG GTG TGA ACG GAT TTG-3′ and reverse 5′-TGT AGA CCA TGT AGT TGA GGT CA-3′). The following PCR conditions were applied for PCR amplification: tyrosinase and MITF: 25 cycles of denaturation at 95 °C for 45 s, annealing at 62 °C for 45 s and extended at 72 °C for 1 min; GAPDH 23 cycles of denaturation at 94 °C for 30 s, annealing at 60 °C for 30 s and extended at 72 °C for 30 s. GAPDH was used as an internal control to evaluate relative expression of MITF and tyrosinase.

4.7. Protein Extraction and Western Blotting Analysis

B16F10 melanoma cells were cultured at a density of 1 × 104 cells/mL in 6 well plate overnight. Then, the cells were treated with the indicated concentrations of fisetin for 96 h and lysed with PRO-PREP lysis buffer (iNtRON Biotechnology). In a parallel experiment, the cells were washed with ice-cold PBS, and the cytosolic and nuclear protein was extracted using NE-PERTM Nuclear and Cytoplasmic Extraction Reagents (Pierce, Rockford, IL, USA). After cleaning lysates by centrifugation, protein was quantified by the Bio-Rad protein assay reagents (Bio-Rad, Hercules, CA, USA). An equal amount of protein was separated by SDS-polyacrylamide gel, transferred onto nitrocellulose membrane (Schleicher & Schuell, Keene, NH, USA) and then immunoblotted with the indicated antibodies. The expressional value was normalized to the intensity of β-actin or nucleolin.

4.8. In Vivo Melanogenic Effect in Zebrafish Larvae

Zebrafish was raised and handled according to standard guidelines of the Animal Care and Use Committee of Jeju National University (approval No.: 2019-0052, 12/18/2019). All the zebrafish-related experiments are performed as previously described method [63]. In brief, inbreed AB strain of zebrafish was mated and the eggs were collected. The eggs were kept in E3 embryo media for 24 h and manually removed the chorion and treated the chemical for 72 h. Images were taken by Olympus SZ2-ILST stereomicroscope (Tokyo, Japan) and the heart rate of zebrafish was also measured to evaluate cardiotoxicity of fisetin. In order to evaluate the effect of fisetin in the presence of α-MSH, after dechorionation, zebrafish larvae were treated with 200 μM PTU for 24 h to remove all the pigments. Then, the larvae were washed with E3 embryo media and treated with 1 µg/mL α-MSH for 2 h prior to fisetin treatment for an additional 72 h.

4.9. Determination of Cardiotoxicity in Zebrafish

The cardiotoxicity of fisetin was determined by comparing the heart rate of zebrafish larvae, because monitoring the zebrafish heart rate is a great tool in drug development and cardiotoxicity study [64,65]. Briefly, zebrafish larvae were placed under a stereomicroscope (Olympus SZ2-ILST) for 4 min at room temperature for allowing embryos to acclimate to the light. The heart rate was calculated by counting the number of heart beats in 1 min. The obtained results were expressed as average heart rate per min.

4.10. Molecular Docking Prediction

Recombinant mushroom tyrosinase (PDB ID: 5M6B) and GSK-3β (PDB ID: 1J1B) were obtained from RCSB protein database bank (PDB, http://www.rcsb.org), and canonical SMILES of fisetin and other chemicals such as tideglusib, enzastaurin, and LY2090314 were obtained from PubChem (https://pubchem.ncbi.nlm.nih.gov). Then, molecular docking score was calculated using mcule with Autodock vina. Four docking poses were provided and representative images were displayed using UCSF Chimera (https://www.cgl.ucsf.edu/chimera). The UCSF Chimera predicted active hydrogen binding to amino acids and distance.

4.11. Statistical Analysis

The images for RT-PCR and western blotting analysis were visualized by Chemi-Smart 2000 (Vilber Lourmat, Marne-la-Vallee, France). Each image was captured using Chemi-Capt (Vilber Lourmat) and transported into Adobe Photoshop. Images of zebrafish larvae were taken by Olympus SZ2-ILST stereomicroscope. All bands were quantified by Image J software (Wayne Rasband, National Institute of Health) and then statistically analyzed by Sigma plot 12.0. All data are presented as the mean ± the standard error of the median (SEM). Significant differences between groups were determined using an unpaired one-way ANOVA with Bonferroni correction. Values of ***, p < 0.001, **, p < 0.01, and *, p < 0.05 were considered to indicate statistical significance. The results shown in each of the figures are representative of at least three independent experiments.