Literature DB >> 318763

Novel screening procedure for recombinant plasmids.

J Telford, P Boseley, W Schaffner, M Birnstiel.   

Abstract

Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 318763     DOI： 10.1126/science.318763

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  16 in total

1.  Isolation of Plasmid DNA from Butyrivibrio fibrisolvens.

Authors:  R M Teather
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.

Authors:  S Mickel; V Arena; W Bauer
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

Review 4.  Molecular cloning of DNA. An introduction into techniques and problems.

Authors:  H P Vosberg
Journal:  Hum Genet       Date:  1977-12-29       Impact factor: 4.132

5.  Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.

Authors:  P K Umeda; A M Sinha; S Jakovcic; S Merten; H J Hsu; K N Subramanian; R Zak; M Rabinowitz
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

6.  Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.

Authors:  P E Berg
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

7.  Construction of a cDNA clone corresponding to mouse alpha 1(IV) procollagen.

Authors:  C L dos Santos; L L Villa; S Sonohara; R R Brentani
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

8.  Rapid procedure for the detection of plasmids in Staphylococcus epidermidis.

Authors:  C R Wilson; P A Totten; J N Baldwin
Journal:  Appl Environ Microbiol       Date:  1978-08       Impact factor: 4.792

9.  Sequence-specific DNA uptake in Haemophilus transformation.

Authors:  K L Sisco; H O Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

10.  Propagation of restriction fragments from the mitochondrial DNA of Saccharomyces cerevisiae in E. coli by means of plasmid vectors.

Authors:  P E Berg; A Lewin; T Christianson; M Rabinowitz
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.