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Literature DB >> 6546618

Construction of a cDNA clone corresponding to mouse alpha 1(IV) procollagen.

C L dos Santos, L L Villa, S Sonohara, R R Brentani.

Abstract

A new procedure for the synthesis of double stranded cDNA, based upon release of mRNA by "in vitro" translation, was used to clone type IV collagen. Collagen synthesizing polysomes selectively isolated from a mouse parietal yolk sac carcinoma (PYS-2) were used for translation in an heterologous cell-free system. Translation products were collagenase-sensitive and displayed an electrophoretic mobility correspondent to type IV collagen. Translation released mRNA was employed to construct a 100 base pairs long cDNA clone which hybridized to a 7,800 nucleotides long mRNA. Peptides synthesized by "in vitro" translation of hybrid selected mRNA displayed an electrophoretic mobility compatible with that of alpha 1 (IV) collagen, were sensitive to collagenase and were immunoprecipitated by anti-type IV collagen antibody.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1984 PMID： 6546618 PMCID： PMC318638 DOI： 10.1093/nar/12.4.2035

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

31 in total

1. Translation of chick calvarial procollagen messenger RNA'S by a messenger RNA dependent reticulocyte lysate.

Authors: J M Monson; H M Goodman
Journal: Biochemistry Date: 1978-11-28 Impact factor: 3.162

2. Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis.

Authors: D W Rowe; R C Moen; J M Davidson; P H Byers; P Bornstein; R D Palmiter
Journal: Biochemistry Date: 1978-05-02 Impact factor: 3.162

3. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP.

Authors: T F Walseth; R A Johnson
Journal: Biochim Biophys Acta Date: 1979-03-28

4. Heterogeneity of pepsin-solubilized human glomerular basement membrane collagen.

Authors: K Tryggvason; K I Kivirikko
Journal: Nephron Date: 1978 Impact factor: 2.847

5. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.

Authors: B Peterkofsky; R Diegelmann
Journal: Biochemistry Date: 1971-03-16 Impact factor: 3.162

Construction of a cDNA clone corresponding to mouse alpha 1(IV) procollagen.

1. Translation of chick calvarial procollagen messenger RNA'S by a messenger RNA dependent reticulocyte lysate.

2. Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis.

3. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP.

4. Heterogeneity of pepsin-solubilized human glomerular basement membrane collagen.

5. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.

6. Isolation and characterization of two collagenous components from anterior lens capsule.

7. Isolation of a collagen from basement membranes containing three identical - chains.

8. The effect of ribonuclease on rat-liver ribosomes.

9. Construction of a recombinant bacterial plasmid containing a chick pro-alpha2 collagen gene sequence.

10. Isolation of cDNA clones for basal lamina components: type IV procollagen.

Review 1. Collagen genes and inherited connective tissue disease.