Literature DB >> 31854058

An Activity-Based Sensing Approach for the Detection of Cyclooxygenase-2 in Live Cells.

Anuj K Yadav1, Christopher J Reinhardt1, Andres S Arango2, Hannah C Huff1, Liang Dong3, Michael G Malkowski3, Aditi Das2,4, Emad Tajkhorshid1,2, Jefferson Chan1.   

Abstract

Cyclooxygenase-2 (COX-2) overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to account for and illuminate the effect of the local environments on protein activity. Herein, we report the development of CoxFluor, the first activity-based sensing approach for monitoring COX-2 within live cells with confocal microscopy and flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen-dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells.
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  activity-based sensing; bioimaging; fluorescent probes; isoform selectivity; molecular dynamics

Year:  2020        PMID： 31854058      PMCID： PMC7416425          DOI： 10.1002/anie.201914845

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  86 in total

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Journal:  J Nucl Med       Date:  2002-01       Impact factor: 10.057

2.  Detection of overexpressed COX-2 in precancerous lesions of hamster pancreas and lungs by molecular imaging: implications for early diagnosis and prevention.

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Review 3.  Cyclooxygenase in biology and disease.

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4.  Effect of regulated expression of human cyclooxygenase isoforms on eicosanoid and isoeicosanoid production in inflammation.

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5.  Partnering between monomers of cyclooxygenase-2 homodimers.

Authors:  Chong Yuan; Caroline Jill Rieke; Gilad Rimon; Byron A Wingerd; William L Smith
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-10       Impact factor: 11.205

6.  Characterization of celecoxib and valdecoxib binding to cyclooxygenase.

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Journal:  Mol Pharmacol       Date:  2003-04       Impact factor: 4.436

Review 7.  Cyclooxygenases: structural and functional insights.

Authors:  Carol A Rouzer; Lawrence J Marnett
Journal:  J Lipid Res       Date:  2008-10-23       Impact factor: 5.922

8.  An off-on COX-2-specific fluorescent probe: targeting the Golgi apparatus of cancer cells.

Authors:  Hua Zhang; Jiangli Fan; Jingyun Wang; Shuangzhe Zhang; Bairui Dou; Xiaojun Peng
Journal:  J Am Chem Soc       Date:  2013-07-29       Impact factor: 15.419

9.  Involvement of arginine 120, glutamate 524, and tyrosine 355 in the binding of arachidonate and 2-phenylpropionic acid inhibitors to the cyclooxygenase active site of ovine prostaglandin endoperoxide H synthase-1.

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Journal:  J Biol Chem       Date:  1996-01-26       Impact factor: 5.157

10.  Arginine 120 of prostaglandin G/H synthase-1 is required for the inhibition by nonsteroidal anti-inflammatory drugs containing a carboxylic acid moiety.

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  8 in total

Review 1.  Advances in Activity-Based Sensing Probes for Isoform-Selective Imaging of Enzymatic Activity.

Authors:  Sarah H Gardner; Christopher J Reinhardt; Jefferson Chan
Journal:  Angew Chem Int Ed Engl       Date:  2020-09-11       Impact factor: 15.336

Review 2.  Targeted contrast agents and activatable probes for photoacoustic imaging of cancer.

Authors:  Zhenxiang Zhao; Chelsea B Swartchick; Jefferson Chan
Journal:  Chem Soc Rev       Date:  2022-02-07       Impact factor: 60.615

Review 3.  Lessons in Organic Fluorescent Probe Discovery.

Authors:  Sachin B Wagh; Vladimir A Maslivetc; James J La Clair; Alexander Kornienko
Journal:  Chembiochem       Date:  2021-06-23       Impact factor: 3.164

Review 4.  Activated molecular probes for enzyme recognition and detection.

Authors:  Meng Yuan; Ying Wu; Caiyan Zhao; Zhongxiang Chen; Lichao Su; Huanghao Yang; Jibin Song
Journal:  Theranostics       Date:  2022-01-01       Impact factor: 11.556

5.  Light-up photoluminescence sensing of a nerve agent simulant by a bis-porphyrin-salen-UO2 complex.

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Journal:  RSC Adv       Date:  2021-04-07       Impact factor: 3.361

6.  Activity-Based NIR Bioluminescence Probe Enables Discovery of Diet-Induced Modulation of the Tumor Microenvironment via Nitric Oxide.

Authors:  Anuj K Yadav; Michael C Lee; Melissa Y Lucero; Shengzhang Su; Christopher J Reinhardt; Jefferson Chan
Journal:  ACS Cent Sci       Date:  2022-03-16       Impact factor: 18.728

7.  A molecular-logic gate for COX-2 and NAT based on conformational and structural changes: visualizing the progression of liver disease.

Authors:  Yuehua Chen; Yuzhu Wang; Yonggang Yang; Yuhuan Li; Yafu Wang; Ge Wang; Tony D James; Xiaopeng Xuan; Hua Zhang; Yufang Liu
Journal:  Chem Sci       Date:  2020-05-25       Impact factor: 9.825

8.  A dual-caged resorufin probe for rapid screening of infections resistant to lactam antibiotics.

Authors:  Jinghang Xie; Ran Mu; Mingxi Fang; Yunfeng Cheng; Fiona Senchyna; Angel Moreno; Niaz Banaei; Jianghong Rao
Journal:  Chem Sci       Date:  2021-05-28       Impact factor: 9.825
  8 in total

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