PURPOSE: Herein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation. METHODS: Magnetic resonance imaging was performed on room-temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T1 mapping and T 2 ∗ -weighted images were acquired using sweep imaging with Fourier transformation and gradient-echo sequences, respectively. Verification of IONP localization was performed through histological assessment and microcomputer tomography. RESULTS: Quantitative imaging was achieved for organs and VCAs perfused with up to 642 mMFe (36 mgFe /mL), which is above previous demonstrations of upper limit detection in agarose (35.7mMFe [2 mgFe /mL]). The stability of IONPs in the perfusate had an effect on the quality of distribution and imaging within organs or VCA. Finally, MRI provided more accurate IONP localization than Prussian blue histological staining in this system, wherein IONPs remain primarily in the vasculature. CONCLUSION: Using MRI, we were able to assess the distribution of IONPs throughout organs and VCAs varying in complexity. Additional studies are necessary to better understand this system and validate the calibration between T1 measurements and IONP concentration.
PURPOSE: Herein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation. METHODS: Magnetic resonance imaging was performed on room-temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T1 mapping and T 2 ∗ -weighted images were acquired using sweep imaging with Fourier transformation and gradient-echo sequences, respectively. Verification of IONP localization was performed through histological assessment and microcomputer tomography. RESULTS: Quantitative imaging was achieved for organs and VCAs perfused with up to 642 mMFe (36 mgFe /mL), which is above previous demonstrations of upper limit detection in agarose (35.7mMFe [2 mgFe /mL]). The stability of IONPs in the perfusate had an effect on the quality of distribution and imaging within organs or VCA. Finally, MRI provided more accurate IONP localization than Prussian blue histological staining in this system, wherein IONPs remain primarily in the vasculature. CONCLUSION: Using MRI, we were able to assess the distribution of IONPs throughout organs and VCAs varying in complexity. Additional studies are necessary to better understand this system and validate the calibration between T1 measurements and IONP concentration.
Authors: Jinjin Zhang; Hattie L Ring; Katie R Hurley; Qi Shao; Cathy S Carlson; Djaudat Idiyatullin; Navid Manuchehrabadi; P Jack Hoopes; Christy L Haynes; John C Bischof; Michael Garwood Journal: Magn Reson Med Date: 2016-09-25 Impact factor: 4.668
Authors: Pj Hoopes; Rr Strawbridge; Uj Gibson; Q Zeng; Ze Pierce; M Savellano; Ja Tate; Ja Ogden; I Baker; R Ivkov; Ar Foreman Journal: Proc SPIE Int Soc Opt Eng Date: 2007-02-13
Authors: Jinjin Zhang; Ryan Chamberlain; Michael Etheridge; Djaudat Idiyatullin; Curtis Corum; John Bischof; Michael Garwood Journal: Magn Reson Med Date: 2014-03-24 Impact factor: 4.668
Authors: Joseph F Poduslo; Thomas M Wengenack; Geoffry L Curran; Thomas Wisniewski; Einar M Sigurdsson; Slobodon I Macura; Bret J Borowski; Clifford R Jack Journal: Neurobiol Dis Date: 2002-11 Impact factor: 5.996
Authors: Zhe Gao; Baterdene Namsrai; Zonghu Han; Purva Joshi; Joseph Sushil Rao; Vasanth Ravikumar; Anirudh Sharma; Hattie L Ring; Djaudat Idiyatullin; Elliott C Magnuson; Paul A Iaizzo; Elena G Tolkacheva; Michael Garwood; Yoed Rabin; Michael Etheridge; Erik B Finger; John C Bischof Journal: Adv Mater Technol Date: 2021-10-01
Authors: Anirudh Sharma; Joseph Sushil Rao; Zonghu Han; Lakshya Gangwar; Baterdene Namsrai; Zhe Gao; Hattie L Ring; Elliott Magnuson; Michael Etheridge; Brian Wowk; Gregory M Fahy; Michael Garwood; Erik B Finger; John C Bischof Journal: Adv Sci (Weinh) Date: 2021-08-11 Impact factor: 16.806