Azin Abazari1, Harbans S Dhadwal2, John Wittpenn3. 1. Department of Ophthalmology, SUNY Stony Brook, Stony Brook, NY, USA. 2. Integrated Fiber Optic Systems, Inc, Stony Brook, NY, USA. 3. Ophthalmic Consultants of Long Island, East Setauket, NY, USA.
Abstract
PURPOSE: Preventing or delaying the onset of presbyopia and cataract formation remains a challenge. The goal of this study was to establish the utility of the Vision Index Pen (VIP), designed to measure in vivo dynamic light scattering (DLS) from the crystalline lens, in the detection of early cataract or loss of accommodation and to show reproducibility through trials at two independent sites. The gradual loss of transparency of the lens was characterized by the lens crystallin aggregation index (LCX) derived from measured DLS data. METHODS: Volunteers in different age groups participated in two independently operated observational clinical studies. All subjects underwent a detailed eye exam and VIP measurement of the intensity correlation of the backscattered light from the lens. RESULTS: LCX values extracted from DLS data show strong correlation with the aging lens, ranging from 20 at the age of 20 years to over 150 at 60 years. Quantitatively significant changes in the LCX value occur from 35 years to 55 years. LCX values were found to correlate with the loss of accommodation (correlation of -0.563 with P < 0.001) and with published data regarding the change in relative lens resistance with age. CONCLUSIONS: Results from two independent observational clinical trials have confirmed the repeatability and reproducibility of the VIP diagnostic device as a viable clinical tool for tracking localized macromolecular changes taking place in the aging lens. Detection of early changes in the crystalline lens can be useful in developing patient-specific prediction models, which can be used to screen patients who may benefit from early therapeutic interventions for delaying the onset of presbyopia and cataract growth. TRANSLATIONAL RELEVANCE: The VIP diagnostic device provides in vivo access to the human lens, enabling characterization of the unfolding and decomposition of long-lived macromolecules. Copyright 2019 The Authors.
PURPOSE: Preventing or delaying the onset of presbyopia and cataract formation remains a challenge. The goal of this study was to establish the utility of the Vision Index Pen (VIP), designed to measure in vivo dynamic light scattering (DLS) from the crystalline lens, in the detection of early cataract or loss of accommodation and to show reproducibility through trials at two independent sites. The gradual loss of transparency of the lens was characterized by the lens crystallin aggregation index (LCX) derived from measured DLS data. METHODS: Volunteers in different age groups participated in two independently operated observational clinical studies. All subjects underwent a detailed eye exam and VIP measurement of the intensity correlation of the backscattered light from the lens. RESULTS: LCX values extracted from DLS data show strong correlation with the aging lens, ranging from 20 at the age of 20 years to over 150 at 60 years. Quantitatively significant changes in the LCX value occur from 35 years to 55 years. LCX values were found to correlate with the loss of accommodation (correlation of -0.563 with P < 0.001) and with published data regarding the change in relative lens resistance with age. CONCLUSIONS: Results from two independent observational clinical trials have confirmed the repeatability and reproducibility of the VIP diagnostic device as a viable clinical tool for tracking localized macromolecular changes taking place in the aging lens. Detection of early changes in the crystalline lens can be useful in developing patient-specific prediction models, which can be used to screen patients who may benefit from early therapeutic interventions for delaying the onset of presbyopia and cataract growth. TRANSLATIONAL RELEVANCE: The VIP diagnostic device provides in vivo access to the human lens, enabling characterization of the unfolding and decomposition of long-lived macromolecules. Copyright 2019 The Authors.
Authors: Joan A Martínez-Roda; Meritxell Vilaseca; Juan C Ondategui; Anna Giner; Francisco J Burgos; Genís Cardona; Jaume Pujol Journal: Clin Exp Optom Date: 2010-11-17 Impact factor: 2.742