Ellen Cristine Duarte Garcia1, Ana Carolina Rossaneis2, Alexandre Salvatore Pipino1, Gustavo Vasconcelos Gomes1, Fábio de Rezende Pinna3, Richard Louis Voegels3, Richard L Doty4, Waldiceu Aparecido Verri2, Marco Aurélio Fornazieri5,6,7. 1. Department of Clinical Surgery, Londrina State University (UEL), Londrina, PR, Brazil. 2. Laboratory of Pain, Inflammation, Neuropathy and Cancer, Department of Pathological Sciences, UEL, Londrina, PR, Brazil. 3. Department of Otorhinolaryngology, University of São Paulo, São Paulo, SP, Brazil. 4. Smell and Taste Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. 5. Department of Clinical Surgery, Londrina State University (UEL), Londrina, PR, Brazil. marcofornazieri@gmail.com. 6. Department of Otorhinolaryngology, University of São Paulo, São Paulo, SP, Brazil. marcofornazieri@gmail.com. 7. Department of Medicine, Pontifical Catholic University of Paraná, Londrina, Brazil. marcofornazieri@gmail.com.
Abstract
PURPOSE: There is no standardized approach for preserving olfactory function in the side of the nose where biopsy of the olfactory epithelium (OE) is performed. Moreover, a gold standard technique for obtaining human OE in vivo is still lacking. We determined the efficacy of obtaining good-quality OE specimens suitable for pathological analysis from the lower half of the superior turbinate and verified the safety of this procedure in maintaining bilateral and unilateral olfactory function. METHODS: In 21 individuals without olfactory complaints and who had undergone septoplasty and inferior turbinectomy OE biopsy was made during septoplasty. Olfactory function, both unilateral and bilateral, was assessed using the University of Pennsylvania Smell Identification Test (UPSIT) before and 1 month after the procedure. Specimens were marked with the olfactory marker protein for confirmation of OE presence. RESULTS: Ninety percent of the samples contained OE, although clear histological characterization was possible from only 62%. There was no deterioration of UPSIT scores either bilaterally or unilaterally on the side of the biopsy. Patients also maintained the ability to identify individual odorants. CONCLUSION: Biopsies of the lower half of the superior turbinate do not affect olfactory function and show strong efficacy in yielding OE tissue and moderate efficacy for yielding tissue appropriate for morphological analysis. Future studies are needed to assess the safety of this procedure in other OE regions.
PURPOSE: There is no standardized approach for preserving olfactory function in the side of the nose where biopsy of the olfactory epithelium (OE) is performed. Moreover, a gold standard technique for obtaining human OE in vivo is still lacking. We determined the efficacy of obtaining good-quality OE specimens suitable for pathological analysis from the lower half of the superior turbinate and verified the safety of this procedure in maintaining bilateral and unilateral olfactory function. METHODS: In 21 individuals without olfactory complaints and who had undergone septoplasty and inferior turbinectomy OE biopsy was made during septoplasty. Olfactory function, both unilateral and bilateral, was assessed using the University of Pennsylvania Smell Identification Test (UPSIT) before and 1 month after the procedure. Specimens were marked with the olfactory marker protein for confirmation of OE presence. RESULTS: Ninety percent of the samples contained OE, although clear histological characterization was possible from only 62%. There was no deterioration of UPSIT scores either bilaterally or unilaterally on the side of the biopsy. Patients also maintained the ability to identify individual odorants. CONCLUSION: Biopsies of the lower half of the superior turbinate do not affect olfactory function and show strong efficacy in yielding OE tissue and moderate efficacy for yielding tissue appropriate for morphological analysis. Future studies are needed to assess the safety of this procedure in other OE regions.
Authors: Adrien A Eshraghi; Mehdi Mirsaeidi; Camron Davies; Fred F Telischi; Nirupa Chaudhari; Rahul Mittal Journal: Front Physiol Date: 2020-09-16 Impact factor: 4.566
Authors: Ellen Cristine Duarte Garcia; Lucas de Almeida Luz; Lucas Kanieski Anzolin; José Lucas Barbosa da Silva; Richard L Doty; Fábio de Rezende Pinna; Richard Louis Voegels; Marco Aurélio Fornazieri Journal: Braz J Otorhinolaryngol Date: 2021-06-05