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Literature DB >> 3169007

The functional significance of DNA sequence structure in a site-specific genetic recombination reaction.

Abstract

A series of sequence changes in the spacer region of the FLP recombination target (FRT) site are presented which drastically reduce site function without affecting recognition by the FLP protein. The effects follow a pattern which indicates that two structural features of the FRT site are essential for site function: a pair of pyrimidine tracts arranged in a palindrome and a predominance of AT base pairs in the spacer. The FRT site represents a sequence that serves to facilitate unwinding of the DNA within the spacer region during recombination. The results provide a clear demonstration of a role for a DNA sequence element that is distinct from protein recognition.

Entities: Chemical

Mesh：

Substances：

Year: 1988 PMID： 3169007 PMCID： PMC457177 DOI： 10.1002/j.1460-2075.1988.tb03017.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

34 in total

1. Three-dimensional structure of a complex of antibody with influenza virus neuraminidase.

Authors: P M Colman; W G Laver; J N Varghese; A T Baker; P A Tulloch; G M Air; R G Webster
Journal: Nature Date: 1987 Mar 26-Apr 1 Impact factor: 49.962

2. The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.

Authors: R M Gronostajski; P D Sadowski
Journal: Mol Cell Biol Date: 1985-11 Impact factor: 4.272

3. Specific contacts between the FLP protein of the yeast 2-micron plasmid and its recombination site.

Authors: R C Bruckner; M M Cox
Journal: J Biol Chem Date: 1986-09-05 Impact factor: 5.157

4. Crystalline A-dna: the X-ray analysis of the fragment d(G-G-T-A-T-A-C-C).

Authors: Z Shakked; D Rabinovich; W B Cruse; E Egert; O Kennard; G Sala; S A Salisbury; M A Viswamitra
Journal: Proc R Soc Lond B Biol Sci Date: 1981-11-24

5. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.

Authors: A H Wang; G J Quigley; F J Kolpak; J L Crawford; J H van Boom; G van der Marel; A Rich
Journal: Nature Date: 1979-12-13 Impact factor: 49.962

6. Site-specific recombination promotes plasmid amplification in yeast.

Authors: F C Volkert; J R Broach
Journal: Cell Date: 1986-08-15 Impact factor: 41.582

7. Cationic metals promote sequence-directed DNA bending.

Authors: C H Laundon; J D Griffith
Journal: Biochemistry Date: 1987-06-30 Impact factor: 3.162

8. Mechanism of damage recognition by Escherichia coli DNA photolyase.

Authors: I Husain; G B Sancar; S R Holbrook; A Sancar
Journal: J Biol Chem Date: 1987-09-25 Impact factor: 5.157

9. Homology-dependent interactions in phage lambda site-specific recombination.

Authors: P A Kitts; H A Nash
Journal: Nature Date: 1987 Sep 24-30 Impact factor: 49.962

10. Nuclease S1-sensitive sites in multigene families: human U2 small nuclear RNA genes.

Authors: H Htun; E Lund; G Westin; U Pettersson; J E Dahlberg
Journal: EMBO J Date: 1985-07 Impact factor: 11.598

14 in total

1. A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.

Authors: Yuri Voziyanov; A Francis Stewart; Makkuni Jayaram
Journal: Nucleic Acids Res Date: 2002-04-01 Impact factor: 16.971

The functional significance of DNA sequence structure in a site-specific genetic recombination reaction.

1. Three-dimensional structure of a complex of antibody with influenza virus neuraminidase.

2. The FLP recombinase of the Saccharomyces cerevisiae 2 microns plasmid attaches covalently to DNA via a phosphotyrosyl linkage.

3. Specific contacts between the FLP protein of the yeast 2-micron plasmid and its recombination site.

4. Crystalline A-dna: the X-ray analysis of the fragment d(G-G-T-A-T-A-C-C).

5. Molecular structure of a left-handed double helical DNA fragment at atomic resolution.

6. Site-specific recombination promotes plasmid amplification in yeast.

7. Cationic metals promote sequence-directed DNA bending.

8. Mechanism of damage recognition by Escherichia coli DNA photolyase.

9. Homology-dependent interactions in phage lambda site-specific recombination.

10. Nuclease S1-sensitive sites in multigene families: human U2 small nuclear RNA genes.

1. A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.

2. A bacterial model system for chromosomal targeting.

3. Mechanisms of insertional mutagenesis in human genes causing genetic disease.

4. Recommended Method for Chromosome Exploitation: RMCE-based Cassette-exchange Systems in Animal Cell Biotechnology.

5. Characterization of Holliday structures in FLP protein-promoted site-specific recombination.

6. Holliday junctions in FLP recombination: resolution by step-arrest mutants of FLP protein.

7. Holliday intermediates and reaction by-products in FLP protein-promoted site-specific recombination.

8. SSV1-encoded site-specific recombination system in Sulfolobus shibatae.

9. Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo.

10. Chemical probe and missing nucleoside analysis of Flp recombinase bound to the recombination target sequence.