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Literature DB >> 3054874

Holliday junctions in FLP recombination: resolution by step-arrest mutants of FLP protein.

M Jayaram¹, K L Crain, R L Parsons, R M Harshey.

Abstract

The FLP "recombinase" of the 2-micron circle yeast plasmid can resolve synthetic FLP site-Holliday junctions. Mutants of the FLP protein that are blocked in recombination but are normal in substrate cleavage can also mediate resolution. The products of resolution by these mutants are almost exclusively nicked molecules with a protein-bound 3' end. There is no significant asymmetry in strand cleavage (top versus bottom) by the mutants in linear or in circular FLP substrates; nor is there a bias in resolution (toward parentals or toward recombinants) of Holliday junctions (corresponding to top- or to bottom-strand exchange) by wild-type FLP. During normal FLP recombination, a small amount of the expected Holliday intermediate can be detected.

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Year: 1988 PMID： 3054874 PMCID： PMC282308 DOI： 10.1073/pnas.85.21.7902

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

Review 8. All change at Holliday junction.

Authors: D M Lilley
Journal: Proc Natl Acad Sci U S A Date: 1997-09-02 Impact factor: 11.205

9. Selection of novel, specific single-stranded DNA sequences by Flp, a duplex-specific DNA binding protein.

Authors: X D Zhu; P D Sadowski
Journal: Nucleic Acids Res Date: 1998-03-01 Impact factor: 16.971

10. The stereochemistry of a four-way DNA junction: a theoretical study.

Authors: E von Kitzing; D M Lilley; S Diekmann
Journal: Nucleic Acids Res Date: 1990-05-11 Impact factor: 16.971

Holliday junctions in FLP recombination: resolution by step-arrest mutants of FLP protein.

1. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site.

2. Occurrence of crossed strand-exchange forms in yeast DNA during meiosis.

3. The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences.

4. Preparative and analytical purification of DNA from agarose.

5. Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination.

6. Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage lambda.

7. int-h: An int mutation of phage lambda that enhances site-specific recombination.

8. Structure and function of the phage lambda att site: size, int-binding sites, and location of the crossover point.

9. Nucleotide sequence of the yeast plasmid.

10. The integrase family of site-specific recombinases: regional similarities and global diversity.

1. Domain of a yeast site-specific recombinase (Flp) that recognizes its target site.

2. Viewing single lambda site-specific recombination events from start to finish.

3. Synapsis, strand scission, and strand exchange induced by the FLP recombinase: analysis with half-FRT sites.

4. Characterization of Holliday structures in FLP protein-promoted site-specific recombination.

5. A histone octamer blocks branch migration of a Holliday junction.

6. Similarities and differences among 105 members of the Int family of site-specific recombinases.

7. Near-simultaneous DNA cleavage by the subunits of the junction-resolving enzyme T4 endonuclease VII.

Review 8. All change at Holliday junction.

9. Selection of novel, specific single-stranded DNA sequences by Flp, a duplex-specific DNA binding protein.

10. The stereochemistry of a four-way DNA junction: a theoretical study.