Olive Tang1, Elizabeth Selvin2,3, Valerie Arends4, Amy Saenger4. 1. Johns Hopkins Bloomberg School of Public Health, Baltimore, MD. 2. Johns Hopkins Bloomberg School of Public Health, Baltimore, MD; eselvin@jhu.edu. 3. Welch Center for Prevention, Epidemiology, and Clinical Research, Baltimore, MD. 4. Advanced Research and Diagnostic Laboratory, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN.
Abstract
BACKGROUND: Complete blood counts (CBCs) are commonly obtained in large multicenter studies. We assessed the stability of 10 parameters after short-term (up to 30 days) frozen storage. METHODS: We compared CBC measurements from fresh samples (n = 53) with samples stored for up to 30 days at -70 °C. We calculated the CVs and intraclass correlation coefficients. RESULTS: Mean values of most parameters, with the exception of hemoglobin and platelet count, were significantly different by 15 days of storage. White blood cell count (CV, 38.3%; 95% CI, 31.3%-46.2%) and red cell distribution width (CV, 37.7%; 95% CI, 34.1%-41.3%) were the most variable. After 30 days, only hemoglobin remained stable and reliable (CV, 0.8%; 95% CI, 0.4%-1.3%). CONCLUSIONS: Hemoglobin remained stable in frozen blood samples stored for up to 30 days at -70 °C and may be reliably used in research studies using short-term frozen specimens. Other CBC parameters measured in stored blood are not sufficiently reliable for research or patient care.
BACKGROUND: Complete blood counts (CBCs) are commonly obtained in large multicenter studies. We assessed the stability of 10 parameters after short-term (up to 30 days) frozen storage. METHODS: We compared CBC measurements from fresh samples (n = 53) with samples stored for up to 30 days at -70 °C. We calculated the CVs and intraclass correlation coefficients. RESULTS: Mean values of most parameters, with the exception of hemoglobin and platelet count, were significantly different by 15 days of storage. White blood cell count (CV, 38.3%; 95% CI, 31.3%-46.2%) and red cell distribution width (CV, 37.7%; 95% CI, 34.1%-41.3%) were the most variable. After 30 days, only hemoglobin remained stable and reliable (CV, 0.8%; 95% CI, 0.4%-1.3%). CONCLUSIONS: Hemoglobin remained stable in frozen blood samples stored for up to 30 days at -70 °C and may be reliably used in research studies using short-term frozen specimens. Other CBC parameters measured in stored blood are not sufficiently reliable for research or patient care.
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