| Literature DB >> 3165705 |
R Pieters1, D R Huismans, A Leyva, A J Veerman.
Abstract
The reduction of the tetrazolium salt MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) to a blue-black formazan product by living but not by dead cells can be used to measure chemosensitivity of tumor cells. The main advantages of the MTT assay are its simplicity, rapidity, and the fact that the results are read automatically with a microplate spectrophotometer. Several reports on the use of the MTT assay in chemosensitivity testing have been published, but all these studies dealt with established cell lines and not with specimens obtained directly from patients. Here we present a study in which the MTT assay has been adapted to assess the effect of antineoplastic drugs on lymphoblasts of children with leukemia.Entities:
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Year: 1988 PMID： 3165705 DOI： 10.1016/0304-3835(88)90294-7
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679