Molecular Detection of Human Salivirus in Italy Through Monitoring of Urban Sewages.

Salivirus (SalV) is a newly discovered virus associated to acute gastroenteritis in humans. In Italy, its prevalence and genetic diversity is unknown. To reduce this knowledge gap, 124 sewage samples collected throughout the country were analyzed for SalV by two nested RT-PCRs targeting the 5'UTR and the 3D regions and by real-time RT-qPCR. Virus RNA was detected in 37 (29.8%) samples; of these, 24 could be characterized and all belonged to genotype A1. Viral concentrations ranged between 2.8 × 103 and 1.9 × 105 genome copies per liter. This is the first report of SalV occurrence in water environments in Italy, suggesting that SalV infection is not uncommon in this country.