Souhaib Aldabbagh1, Isabella Eckerle1, Andreas Müller2, Eric L Delwart3, Anna M Eis-Hübinger4. 1. Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. 2. Dept. of Neonatology and Pediatric Intensive Care, Children's Hospital, University of Bonn Medical Centre, Adenauerallee 119, D-53113 Bonn, Germany. 3. Blood Systems Research Institute, Department of Laboratory Medicine, University of California, 270 Masonic Ave., San Francisco, CA 94118, USA. 4. Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. Electronic address: anna-maria.eis-huebinger@ukb.uni-bonn.de.
Abstract
BACKGROUND: Salivirus (SaV-A) is a novel member of the family Picornaviridae and has been associated with acute gastroenteritis. Recently, a second type of SaV-A, SaV-A2, was identified in a sewage sample from Bangkok, Thailand. No information is available on the prevalence of SaV-A in Western Europe. OBJECTIVES: Stool samples from patients with symptoms of acute viral gastroenteritis were analyzed for SaV-A and the clinical course of SaV-A-positive individuals was evaluated. STUDY DESIGN: A total of 3019 fecal samples collected during 2012-2013 from 1941 hospitalized patients with acute gastroenteritis were screened for SaV-A by a newly designed real-time reverse transcription polymerase chain reaction targeting a conserved sequence in the 5'-untranslated region. Positive results were verified by sequencing the viral capsid protein 1 gene also allowing typing of the virus. Medical records of SaV-A-infected patients were reviewed for clinical features and laboratory data. RESULTS: SaV-A was detected in five patients. Viral RNA concentrations ranged from 7.1×10(6) to 7.2×10(8)copies/g feces. The viruses from four patients were classified as SaV-A1 while SaV-A2 was present in one patient. After reviewing the medical records, SaV-A could not be considered as the sole possible cause of gastroenteritis symptoms given the presence of other plausible causes in all five patients. CONCLUSION: SaV-A infection can be detected in Germany, Western Europe, albeit at low levels. The detection of SaV-A2 in Europe suggests wider spread of SaV-A2. Presence of SaV-A, even at high concentrations, in a stool sample provides no conclusive evidence that SaV is the major cause of the patient's gastroenteritis symptoms.
BACKGROUND: Salivirus (SaV-A) is a novel member of the family Picornaviridae and has been associated with acute gastroenteritis. Recently, a second type of SaV-A, SaV-A2, was identified in a sewage sample from Bangkok, Thailand. No information is available on the prevalence of SaV-A in Western Europe. OBJECTIVES: Stool samples from patients with symptoms of acute viral gastroenteritis were analyzed for SaV-A and the clinical course of SaV-A-positive individuals was evaluated. STUDY DESIGN: A total of 3019 fecal samples collected during 2012-2013 from 1941 hospitalized patients with acute gastroenteritis were screened for SaV-A by a newly designed real-time reverse transcription polymerase chain reaction targeting a conserved sequence in the 5'-untranslated region. Positive results were verified by sequencing the viral capsid protein 1 gene also allowing typing of the virus. Medical records of SaV-A-infectedpatients were reviewed for clinical features and laboratory data. RESULTS:SaV-A was detected in five patients. Viral RNA concentrations ranged from 7.1×10(6) to 7.2×10(8)copies/g feces. The viruses from four patients were classified as SaV-A1 while SaV-A2 was present in one patient. After reviewing the medical records, SaV-A could not be considered as the sole possible cause of gastroenteritis symptoms given the presence of other plausible causes in all five patients. CONCLUSION:SaV-A infection can be detected in Germany, Western Europe, albeit at low levels. The detection of SaV-A2 in Europe suggests wider spread of SaV-A2. Presence of SaV-A, even at high concentrations, in a stool sample provides no conclusive evidence that SaV is the major cause of the patient's gastroenteritis symptoms.
Authors: Jacob D Negrey; John C Mitani; Richard W Wrangham; Emily Otali; Rachna B Reddy; Tressa E Pappas; Kristine A Grindle; James E Gern; Zarin P Machanda; Martin N Muller; Kevin E Langergraber; Melissa Emery Thompson; Tony L Goldberg Journal: Am J Primatol Date: 2022-01-11 Impact factor: 3.014
Authors: Terry Fei Fan Ng; Laura Magaña; Anna Montmayeur; Maria Renee Lopez; Nicole Gregoricus; M Steven Oberste; Jan Vinjé; W Allan Nix Journal: Genome Announc Date: 2016-02-18