Song-Tian Che1, Li Bie2, Xu Li1, Hui Qi1, Peng Yu1, Ling Zuo1. 1. Department of Ocular Fundus Disease, the Second Hospital of Jilin University, Changchun 130022, Jilin Province, China. 2. Department of Neurosurgery, the First Hospital of Jilin University, Changchun 130022, Jilin Province, China.
Abstract
AIM: To explore the effect of parthenolide (PTL) on human uveal melanoma (UM) cells (C918 and SP6.5 cells) and its molecular mechanism. METHODS: Carboxyfluorescein succinimidyl amino ester (CFSE) assays and cell counting kit-8 (CCK-8) were performed to detect the cell viability. Flow cytometry was used to analyze cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays were performed to measure proliferation-related and apoptosis-related factors. RESULTS: Firstly, PTL decreased the viability of C918 and SP6.5 cells in a dose-dependent manner, and the effect of PTL on C918 cells was stronger than on SP6.5; however, it did not affect normal cells. Secondly, PTL increased the proportion of cell number at cell cycle G1 phase in C918 cells, and decreased the proportion of cell number at S phase, but the proportion did not change at G2 phase. In addition, PTL induced the apoptosis of C918 cells, and decreased the expressions of Cyclin D1, B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-XL). Also, PTL increased Cyclin inhibition protein 1 (P21), Bcl-2-associated X protein (Bax), Cysteinyl aspartate specific proteinas-3 (Caspase-3) and Caspase-9 expression. However, the expression of Caspase-8 was not changed. CONCLUSION: PTL inhibites proliferation and induces apoptosis in UM cells by arresting G1 phase and regulating mitochondrial pathway, however, it does not affect normal cells. International Journal of Ophthalmology Press.
AIM: To explore the effect of parthenolide (PTL) on human uveal melanoma (UM) cells (C918 and SP6.5 cells) and its molecular mechanism. METHODS:Carboxyfluorescein succinimidyl amino ester (CFSE) assays and cell counting kit-8 (CCK-8) were performed to detect the cell viability. Flow cytometry was used to analyze cell cycle and apoptosis. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays were performed to measure proliferation-related and apoptosis-related factors. RESULTS: Firstly, PTL decreased the viability of C918 and SP6.5 cells in a dose-dependent manner, and the effect of PTL on C918 cells was stronger than on SP6.5; however, it did not affect normal cells. Secondly, PTL increased the proportion of cell number at cell cycle G1 phase in C918 cells, and decreased the proportion of cell number at S phase, but the proportion did not change at G2 phase. In addition, PTL induced the apoptosis of C918 cells, and decreased the expressions of Cyclin D1, B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-XL). Also, PTL increased Cyclin inhibition protein 1 (P21), Bcl-2-associated X protein (Bax), Cysteinyl aspartate specific proteinas-3 (Caspase-3) and Caspase-9 expression. However, the expression of Caspase-8 was not changed. CONCLUSION:PTL inhibites proliferation and induces apoptosis in UM cells by arresting G1 phase and regulating mitochondrial pathway, however, it does not affect normal cells. International Journal of Ophthalmology Press.
Authors: Laurent Sabbagh; Susan M Kaech; Martin Bourbonnière; Minna Woo; Luchino Y Cohen; Elias K Haddad; Nathalie Labrecque; Rafi Ahmed; Rafick-Pierre Sékaly Journal: J Immunol Date: 2004-11-01 Impact factor: 5.422
Authors: A Kemény-Beke; E Berényi; A Facskó; J Damjanovich; A Horváth; A Bodnár; A Berta; J Aradi Journal: Eur J Ophthalmol Date: 2006 Sep-Oct Impact factor: 2.597
Authors: Anna Wyrębska; Katarzyna Gach; Janusz Szemraj; Karolina Szewczyk; Elżbieta Hrabec; Jacek Koszuk; Tomasz Janecki; Anna Janecka Journal: Chem Biol Drug Des Date: 2011-11-04 Impact factor: 2.817
Authors: Wamidh H Talib; Dima Awajan; Reem Ali Hamed; Aya O Azzam; Asma Ismail Mahmod; Intisar Hadi Al-Yasari Journal: Molecules Date: 2022-08-25 Impact factor: 4.927