| Literature DB >> 31562586 |
Shu Liu1,2, Sibtain Ahmed3, Yaowei Fang4,5,6.
Abstract
α-Amylases are used in various biotechnological processes including the textile, paper, food, biofuels, detergents and pharmaceutical industries. In this study, a novel gene encoding α-amylase was cloned from marine bacterium Salinispora arenicola CNP193 and the protein was expressed in Escherichia coli. The α-amylase gene from S. arenicola CNP193 had a length of 1839 bp and encoded a α-amylase with an estimated molecular mass of 74 kDa. The optimum temperature and pH for the recombinant α-amylase was 50 °C and 7 respectively. Na+, K+ and Ca2+ increased the activity of the recombinant α-amylase whereas the enzyme was inhibited by Cu2+, Zn2+, Hg2+, Pb2+, Fe3+ and Mn2+. Thin layer chromatography results confirmed that monosaccharide, disaccharide and maltotriose are the hydrolysis products. The results of our study suggest that this enzyme has considerable potential in industrial applications.Entities:
Keywords: Biochemical characterization; Expression; Salinispora arenicola; α-Amylase
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Year: 2019 PMID： 31562586 DOI： 10.1007/s10930-019-09870-3
Source DB: PubMed Journal: Protein J ISSN： 1572-3887 Impact factor: 2.371