Anna Prats-Puig1,2, Sílvia Xargay-Torrent1, Robert Feil3, Abel López-Bermejo4,5, Gemma Carreras-Badosa1, Berta Mas-Parés1, Judit Bassols1, Clive J Petry6, Michael Girardot3, Francis D E Zegher7, Lourdes Ibáñez8,9, David B Dunger6. 1. Department of Pediatrics, Girona Institute for Biomedical Research (IDIBGI), Salt, Spain. 2. Department of Physical Therapy, EUSES University of Girona, Salt Girona, Spain. 3. Institute of Molecular Genetics (IGMM), CNRS, University of Montpellier, Montpellier, France. 4. Department of Pediatrics, Girona Institute for Biomedical Research (IDIBGI), Salt, Spain. alopezbermejo@idibgi.org. 5. Department of Pediatrics, Dr. Josep Trueta Hospital, Girona, Spain. alopezbermejo@idibgi.org. 6. Department of Paediatrics, University of Cambridge, Cambridge, UK. 7. Department of Development & Regeneration, University of Leuven, Leuven, Belgium. 8. Endocrinology, Institut de Recerca Pediàtrica Sant Joan de Déu, University of Barcelona, Esplugues, Spain. 9. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), ISCIII, Madrid, Spain.
Abstract
OBJECTIVES: To study DNA methylation at the C19MC locus in the placenta and its association with (1) parental body size, (2) transmission of haplotypes for the C19MC rs55765443 SNP, and (3) offspring's body size and/or body composition at birth and in childhood. SUBJECTS AND METHODS: Seventy-two pregnant women-infant pairs and 63 fathers were included in the study. Weight and height of mothers, fathers and newborns were registered during pregnancy or at birth (n = 72). Placental DNA methylation at the C19MC imprinting control region (ICR) was quantified by bisulfite pyrosequencing. Genotyping of the SNP was performed using restriction fragment length polymorphisms. The children's body size and composition were reassessed at age 6 years (n = 32). RESULTS: Lower levels of placental C19MC methylation were associated with increased body size of mother, specifically with higher pregestational and predelivery weights and height of the mother (β from -0.294 to -0.371; R2 from 0.04 to 0.10 and all p < 0.019), and with higher weight, height, waist and hip circumferences, and fat mass of the child (β from -0.428 to -0.552; R2 from 0.33 to 0.56 and all p < 0.009). Parental transmission of the SNP did not correlate with an altered placental methylation status at the C19MC ICR. CONCLUSIONS: Increased maternal size is associated with reduced placental C19MC methylation, which, in turn, relate to larger body size of the child.
OBJECTIVES: To study DNA methylation at the C19MC locus in the placenta and its association with (1) parental body size, (2) transmission of haplotypes for the C19MC rs55765443 SNP, and (3) offspring's body size and/or body composition at birth and in childhood. SUBJECTS AND METHODS: Seventy-two pregnant women-infant pairs and 63 fathers were included in the study. Weight and height of mothers, fathers and newborns were registered during pregnancy or at birth (n = 72). Placental DNA methylation at the C19MC imprinting control region (ICR) was quantified by bisulfite pyrosequencing. Genotyping of the SNP was performed using restriction fragment length polymorphisms. The children's body size and composition were reassessed at age 6 years (n = 32). RESULTS: Lower levels of placental C19MC methylation were associated with increased body size of mother, specifically with higher pregestational and predelivery weights and height of the mother (β from -0.294 to -0.371; R2 from 0.04 to 0.10 and all p < 0.019), and with higher weight, height, waist and hip circumferences, and fat mass of the child (β from -0.428 to -0.552; R2 from 0.33 to 0.56 and all p < 0.009). Parental transmission of the SNP did not correlate with an altered placental methylation status at the C19MC ICR. CONCLUSIONS: Increased maternal size is associated with reduced placental C19MC methylation, which, in turn, relate to larger body size of the child.
Authors: Carmen Elena Condrat; Valentin Nicolae Varlas; Florentina Duică; Panagiotis Antoniadis; Cezara Alina Danila; Dragos Cretoiu; Nicolae Suciu; Sanda Maria Crețoiu; Silviu Cristian Voinea Journal: Int J Mol Sci Date: 2021-04-09 Impact factor: 5.923