Literature DB >> 3150676

Alpha are more stable than beta anomer oligonucleotides in 3T3 cellular extracts.

S Thenet1, F Morvan, J R Bertrand, C Gautie, C Malvy.   

Abstract

We have compared the stability of alpha and beta anomeric oligonucleotides in NIH 3T3 cellular extracts. We had already shown that alpha are much more resistant than beta oligonucleotides towards purified nucleases. This result is confirmed when using cellular extracts although the difference is smaller. When alpha molecules are combined with an intercalating agent binding in the 3' position a synergistic increase of resistance to degradation is observed.

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Year:  1988        PMID： 3150676     DOI： 10.1016/0300-9084(88)90031-4

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  5 in total

1.  Efficient triple helix formation by oligodeoxyribonucleotides containing alpha- or beta-2-amino-5-(2-deoxy-D-ribofuranosyl) pyridine residues.

Authors:  P J Bates; C A Laughton; T C Jenkins; D C Capaldi; P D Roselt; C B Reese; S Neidle
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

2.  Intracellular distribution of microinjected antisense oligonucleotides.

Authors:  J P Leonetti; N Mechti; G Degols; C Gagnor; B Lebleu
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

3.  alpha-Oligodeoxyribonucleotide N3'-->P5' phosphoramidates: synthesis and duplex formation.

Authors:  K Pongracz; S M Gryaznov
Journal:  Nucleic Acids Res       Date:  1998-02-15       Impact factor: 16.971

4.  Sequence-specific interaction of alpha-beta-anomeric double-stranded DNA with the p50 subunit of NF kappa B: application to the decoy approach.

Authors:  H Tanaka; P Vickart; J R Bertrand; B Rayner; F Morvan; J L Imbach; D Paulin; C Malvy
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971

5.  alpha-DNA X: alpha and beta tetrathymidilates covalently linked to oxazolopyridocarbazolium (OPC): comparative stabilization of oligo beta-[dT]:oligo beta-[dA] and oligo alpha-[dT]:oligo beta-[dA] duplexes by the intercalating agent.

Authors:  D Bazile; C Gautier; B Rayner; J L Imbach; C Paoletti; J Paoletti
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971
  5 in total

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