Literature DB >> 31493371

Efficient data acquisition with three-channel centerpieces in sedimentation velocity.

Kristian Juul-Madsen1, Huaying Zhao2, Thomas Vorup-Jensen3, Peter Schuck4.   

Abstract

Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record data from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting. Published by Elsevier Inc.

Entities:  

Keywords:  3D printing; Analytical ultracentrifugation; Laboratory automation; Sedimentation velocity

Mesh：

Year:  2019        PMID： 31493371      PMCID： PMC6768728          DOI： 10.1016/j.ab.2019.113414

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  37 in total

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5.  Sedimentation velocity analytical ultracentrifugation and SEDFIT/c(s): limits of quantitation for a monoclonal antibody system.

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Journal:  Anal Biochem       Date:  2006-11-28       Impact factor: 3.365

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Journal:  Anal Biochem       Date:  2010-03-03       Impact factor: 3.365

7.  A bayesian approach for quantifying trace amounts of antibody aggregates by sedimentation velocity analytical ultracentrifugation.

Authors:  Patrick H Brown; Andrea Balbo; Peter Schuck
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Authors:  M J Rosovitz; Peter Schuck; Mini Varughese; Arun P Chopra; Varsha Mehra; Yogendra Singh; Lisa M McGinnis; Stephen H Leppla
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9.  On the analysis of sedimentation velocity in the study of protein complexes.

Authors:  Patrick H Brown; Andrea Balbo; Peter Schuck
Journal:  Eur Biophys J       Date:  2009-07-31       Impact factor: 1.733

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Authors:  Randy P Carney; Jin Young Kim; Huifeng Qian; Rongchao Jin; Hakim Mehenni; Francesco Stellacci; Osman M Bakr
Journal:  Nat Commun       Date:  2011-06-07       Impact factor: 14.919
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Authors:  Leonardo M Schuck; Huaying Zhao
Journal:  Anal Biochem       Date:  2020-05-11       Impact factor: 3.365

2.  Analytical Ultracentrifugation (AUC): An Overview of the Application of Fluorescence and Absorbance AUC to the Study of Biological Macromolecules.

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