Literature DB >> 32407733

Resuspending samples in analytical ultracentrifugation.

Leonardo M Schuck1, Huaying Zhao2.   

Abstract

In analytical ultracentrifugation it is often very useful to resuspend samples in situ after sedimentation experiments for further investigation. This can be achieved by manually subjecting the entire sample cell assembly to gentle motion that causes the air bubble in the sample compartment to repeatedly move through the solution and thereby cause convection. Here we describe a cell mixing device that can accomplish the same through axial rotation and slow rocking motion. This cell mixer is low-cost, open-source, and can be easily assembled from readily available components. It can efficiently mix multiple sample cells side-by-side and may be used with various centerpiece designs. Published by Elsevier Inc.

Entities:  

Keywords:  Analytical ultracentrifugation; Laboratory automation; Sedimentation velocity

Year:  2020        PMID: 32407733      PMCID: PMC7398841          DOI: 10.1016/j.ab.2020.113771

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  22 in total

1.  Protein Assembly in Serum and the Differences from Assembly in Buffer.

Authors:  John J Hill; Thomas M Laue
Journal:  Methods Enzymol       Date:  2015-07-14       Impact factor: 1.600

2.  Variable Field Analytical Ultracentrifugation: II. Gravitational Sweep Sedimentation Velocity.

Authors:  Jia Ma; Huaying Zhao; Julia Sandmaier; J Alexander Liddle; Peter Schuck
Journal:  Biophys J       Date:  2016-01-05       Impact factor: 4.033

3.  Enhanced Sample Handling for Analytical Ultracentrifugation with 3D-Printed Centerpieces.

Authors:  Samuel C To; Chad A Brautigam; Sumit K Chaturvedi; Mary T Bollard; Jonathan Krynitsky; John W Kakareka; Thomas J Pohida; Huaying Zhao; Peter Schuck
Journal:  Anal Chem       Date:  2019-04-15       Impact factor: 6.986

4.  An optical alignment system improves precision of soluble aggregate quantitation by sedimentation velocity analytical ultracentrifugation.

Authors:  Brandon L Doyle; Ivan L Budyak; Adam P Rauk; William F Weiss
Journal:  Anal Biochem       Date:  2017-05-19       Impact factor: 3.365

5.  LED based near infrared spectral acquisition for multiwavelength analytical ultracentrifugation: A case study with gold nanoparticles.

Authors:  Joseph Pearson; Helmut Cölfen
Journal:  Anal Chim Acta       Date:  2018-07-11       Impact factor: 6.558

6.  Advanced Multiwavelength Detection in Analytical Ultracentrifugation.

Authors:  Joseph Pearson; Johannes Walter; Wolfgang Peukert; Helmut Cölfen
Journal:  Anal Chem       Date:  2017-12-22       Impact factor: 6.986

7.  Analysis of antibody aggregate content at extremely high concentrations using sedimentation velocity with a novel interference optics.

Authors:  Kristian Schilling; Frank Krause
Journal:  PLoS One       Date:  2015-03-24       Impact factor: 3.240

8.  The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterising the sizes, shapes and interactions of molecules and assemblies in Biochemistry and Polymer Science.

Authors:  Stephen E Harding
Journal:  Eur Biophys J       Date:  2018-07-28       Impact factor: 1.733

9.  A simple cell-alignment protocol for sedimentation velocity analytical ultracentrifugation to complement mechanical and optical alignment procedures.

Authors:  Guy Channell; Vlad Dinu; Gary G Adams; Stephen E Harding
Journal:  Eur Biophys J       Date:  2018-08-29       Impact factor: 1.733

10.  3D-Printing for Analytical Ultracentrifugation.

Authors:  Abhiksha Desai; Jonathan Krynitsky; Thomas J Pohida; Huaying Zhao; Peter Schuck
Journal:  PLoS One       Date:  2016-08-15       Impact factor: 3.240

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  2 in total

1.  Calibrating analytical ultracentrifuges.

Authors:  Huaying Zhao; Ai Nguyen; Samuel C To; Peter Schuck
Journal:  Eur Biophys J       Date:  2021-01-04       Impact factor: 1.733

2.  Quantitative Analysis of Protein Self-Association by Sedimentation Velocity.

Authors:  Huaying Zhao; Wenqi Li; Wendan Chu; Mary Bollard; Regina Adão; Peter Schuck
Journal:  Curr Protoc Protein Sci       Date:  2020-09
  2 in total

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