| Literature DB >> 31478454 |
Dan Zhao1, Cong Shen2,3, Tingting Gao4, Hong Li2, Yueshuai Guo3,5, Feng Li1,6, Chenchen Liu3, Yuanyuan Liu2,3, Xia Chen7, Xi Zhang3, Yangyang Wu3, Yi Yu2, Meng Lin3, Yan Yuan8, Xiaofang Chen1, Xiaoyan Huang3, Shenmin Yang2, Jun Yu7, Jun Zhang3, Bo Zheng2,3.
Abstract
Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis and tumor growth. We have previously identified MTMR7 in the proteomic profile of mouse spermatogonial stem cell (SSC) maturation and differentiation, implying that MTMR7 is associated with neonatal testicular development. In this study, to further explore the distribution and function of MTMR7 in mouse testis, we studied the effect of Mtmr7 knockdown on neonatal testicular development by testicular and SSC culture methods. Our results revealed that MTMR7 is exclusively located in early germ cells. Deficiency of MTMR7 by morpholino in neonatal testis caused excessive SSC proliferation, which was attributable to the aberrant PI3K/AKT signaling activation. Altogether, our study demonstrates that MTMR7 maintains SSC homeostasis by inhibiting PI3K/AKT signaling activation.Entities:
Keywords: Myotubularin related protein 7 (MTMR7); PI3K/AKT signaling; germ cells; spermatogonial stem cell (SSC)
Year: 2019 PMID： 31478454 PMCID： PMC6773228 DOI： 10.1080/15384101.2019.1661174
Source DB: PubMed Journal: Cell Cycle ISSN： 1551-4005 Impact factor: 4.534