Yibo Wu1, Cong Shen2, Tiantian Wu3, Xiaoyan Huang3, Hong Li4, Bo Zheng5. 1. Department of Obstetrics and Gynecology, Affiliated Hospital of Jiangnan University, Wuxi, 214062, China. 2. State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, 215002, China. 3. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China. 4. State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, 215002, China. hongli@njmu.edu.cn. 5. State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, 215002, China. bozheng@njmu.edu.cn.
Abstract
BACKGROUND: Interactions between Sertoli and germ cells are essential for spermatogenesis and male fertility. However, the mechanism of action underlying these interactions in the testes remains largely unknown. In this study, we investigated the distribution and function of syntaxin binding protein 2 (STXBP2) in the mammalian testis. METHODS AND RESULTS: First, we found that STXBP2 was mainly expressed in Sertoli cells. Then, to explore the function of STXBP2 in the testes, we assessed the effects of Stxbp2 knockdown on neonatal testicular and spermatogonial stem cell (SSC) development. Our results revealed that STXBP2 was required for the migration of Sertoli cells and germ cell survival. Mechanistically, we found that STXBP2 interacted with connexin 43 (Cx43) and regulated its expression. CONCLUSIONS: Taken together, our results demonstrated a novel regulatory mechanism in which the STXBP2/Cx43 complex is essential for the maintenance of Sertoli-germline interactions.
BACKGROUND: Interactions between Sertoli and germ cells are essential for spermatogenesis and male fertility. However, the mechanism of action underlying these interactions in the testes remains largely unknown. In this study, we investigated the distribution and function of syntaxin binding protein 2 (STXBP2) in the mammalian testis. METHODS AND RESULTS: First, we found that STXBP2 was mainly expressed in Sertoli cells. Then, to explore the function of STXBP2 in the testes, we assessed the effects of Stxbp2 knockdown on neonatal testicular and spermatogonial stem cell (SSC) development. Our results revealed that STXBP2 was required for the migration of Sertoli cells and germ cell survival. Mechanistically, we found that STXBP2 interacted with connexin 43 (Cx43) and regulated its expression. CONCLUSIONS: Taken together, our results demonstrated a novel regulatory mechanism in which the STXBP2/Cx43 complex is essential for the maintenance of Sertoli-germline interactions.
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