| Literature DB >> 31477243 |
Haroon Butt1, Syed Shan-E-Ali Zaidi2, Norhan Hassan1, Magdy Mahfouz3.
Abstract
Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.Entities:
Keywords: CRISPR-Cas9; CRISPR-directed evolution; HDR; NHEJ; crop breeding; trait engineering
Mesh:
Year: 2019 PMID: 31477243 DOI: 10.1016/j.tibtech.2019.08.001
Source DB: PubMed Journal: Trends Biotechnol ISSN: 0167-7799 Impact factor: 19.536