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Literature DB >> 3147147

Identification of an upstream activation site in the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae.

Abstract

The upstream region of the Saccharomyces cerevisiae pyruvate decarboxylase structural gene, PDC1, has been isolated and fused to the indicator gene Escherichia coli lacZ. 1.2 kb of the upstream region has been sequenced. The PDC1-lacZ fusion has been integrated at the ura3-52 locus in the yeast genome, and has a basal level of expression on ethanol. On glucose media this level is increased 30-50 fold. An upstream activation site, UASpdc, between 793 and 535 bp upstream from the ATG of PDC1, which mediates the response to glucose has been identified by deletion analysis. The UASpdc contains a consensus RPG box, originally identified in ribosomal protein genes (Leer et al. 1985). The function of UASpdc is independent of distance from the ATG. There is also an upstream repressing sequence located between 535 and 385 bp upstream from the translational start of PDC1.

Entities: Chemical Gene Species

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Year: 1988 PMID： 3147147 DOI： 10.1007/bf00521261

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

21 in total

1. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae.

Authors: E Kellermann; P G Seeboth; C P Hollenberg
Journal: Nucleic Acids Res Date: 1986-11-25 Impact factor: 16.971

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Authors: D Shore; K Nasmyth
Journal: Cell Date: 1987-12-04 Impact factor: 41.582

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Authors: C Stanway; J Mellor; J E Ogden; A J Kingsman; S M Kingsman
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

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Authors: A D Gounaris; I Turkenkopf; L L Civerchia; J Greenlie
Journal: Biochim Biophys Acta Date: 1975-10-20

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Authors: K Struhl
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

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Authors: K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

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Authors: H D Schmitt; F K Zimmermann
Journal: J Bacteriol Date: 1982-09 Impact factor: 3.490

8. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.

Authors: A R Buchman; W J Kimmerly; J Rine; R D Kornberg
Journal: Mol Cell Biol Date: 1988-01 Impact factor: 4.272

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Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

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Authors: C L Denis; J Ferguson; E T Young
Journal: J Biol Chem Date: 1983-01-25 Impact factor: 5.157

15 in total

1. Efficient production of L-Lactic acid by metabolically engineered Saccharomyces cerevisiae with a genome-integrated L-lactate dehydrogenase gene.

Authors: Nobuhiro Ishida; Satoshi Saitoh; Kenro Tokuhiro; Eiji Nagamori; Takashi Matsuyama; Katsuhiko Kitamoto; Haruo Takahashi
Journal: Appl Environ Microbiol Date: 2005-04 Impact factor: 4.792

2. Characterisation of the DNA binding domain of the yeast RAP1 protein.

Authors: Y A Henry; A Chambers; J S Tsang; A J Kingsman; S M Kingsman
Journal: Nucleic Acids Res Date: 1990-05-11 Impact factor: 16.971

3. The switch from fermentation to respiration in Saccharomyces cerevisiae is regulated by the Ert1 transcriptional activator/repressor.

Authors: Najla Gasmi; Pierre-Etienne Jacques; Natalia Klimova; Xiao Guo; Alessandra Ricciardi; François Robert; Bernard Turcotte
Journal: Genetics Date: 2014-08-13 Impact factor: 4.562

4. Mutational analysis of the upstream activation site of yeast ribosomal protein genes.

Authors: R T Nieuwint; W H Mager; K C Maurer; R J Planta
Journal: Curr Genet Date: 1989-04 Impact factor: 3.886

5. pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

Authors: P G Seeboth; K Bohnsack; C P Hollenberg
Journal: J Bacteriol Date: 1990-02 Impact factor: 3.490

9. Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites.

Authors: E Boles; F K Zimmermann
Journal: Arch Microbiol Date: 1993 Impact factor: 2.552

10. A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1.

Authors: M S Longtine; N M Wilson; M E Petracek; J Berman
Journal: Curr Genet Date: 1989-10 Impact factor: 3.886