Literature DB >> 3537965

Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae.

E Kellermann, P G Seeboth, C P Hollenberg.   

Abstract

The PDC1 gene of Saccharomyces cerevisiae, encoding pyruvate decarboxylase was sequenced. The gene contains an open reading frame of 1647 base pairs. The codon usage shows the same strong bias as found for some other glycolytic enzymes. Transcription starts mainly at -30 and terminates 100 base pairs downstream of the termination codon. In some strains a second termination site, 46 base pairs upstream of the stop codon was observed. The function of the promoter region was analyzed by fusion to the bacterial structural gene encoding beta-lactamase (bla). On multicopy plasmid or integrated in the genome, the expression of the bla gene showed the regulation of the authentic PDC1 gene.

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Year:  1986        PMID： 3537965      PMCID： PMC311923          DOI： 10.1093/nar/14.22.8963

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Heterogeneity and 5'-terminal structures of the late RNAs of simian virus 40.

Authors:  P K Ghosh; V B Reddy; J Swinscoe; P Lebowitz; S M Weissman
Journal:  J Mol Biol       Date:  1978-12-25       Impact factor: 5.469

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.

Authors:  M Fitzgerald; T Shenk
Journal:  Cell       Date:  1981-04       Impact factor: 41.582

5.  Pyruvate decarboxylase III. Specificity restrictions for thiamine pyrophosphate in the protein association step, sub-unit structure.

Authors:  A D Gounaris; I Turkenkopf; L L Civerchia; J Greenlie
Journal:  Biochim Biophys Acta       Date:  1975-10-20

6.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Proteolysis in eukaryotic cells. Identification of multiple proteolytic enzymes in yeast.

Authors:  T Achstetter; O Emter; C Ehmann; D H Wolf
Journal:  J Biol Chem       Date:  1984-11-10       Impact factor: 5.157

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Isolation and characterization of further cis- and trans-acting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.

Authors:  M Ciriacy
Journal:  Mol Gen Genet       Date:  1979-11
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  28 in total

1.  Molecular cloning of the gene for indolepyruvate decarboxylase from Enterobacter cloacae.

Authors:  J Koga; T Adachi; H Hidaka
Journal:  Mol Gen Genet       Date:  1991-04

2.  Effects of pyruvate decarboxylase overproduction on flux distribution at the pyruvate branch point in Saccharomyces cerevisiae.

Authors:  P van Hoek; M T Flikweert; Q J van der Aart; H Y Steensma; J P van Dijken; J T Pronk
Journal:  Appl Environ Microbiol       Date:  1998-06       Impact factor: 4.792

3.  The glucose-and ethanol-dependent regulation of PDC1 from Saccharomyces cerevisiae are controlled by two distinct promoter regions.

Authors:  E Kellermann; C P Hollenberg
Journal:  Curr Genet       Date:  1988-10       Impact factor: 3.886

4.  Biosynthesis of the peroxisomal dihydroxyacetone synthase from Hansenula polymorpha in Saccharomyces cerevisiae induces growth but not proliferation of peroxisomes.

Authors:  A Gödecke; M Veenhuis; R Roggenkamp; Z A Janowicz; C P Hollenberg
Journal:  Curr Genet       Date:  1989-07       Impact factor: 3.886

5.  pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.

Authors:  P G Seeboth; K Bohnsack; C P Hollenberg
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

6.  Maize pyruvate decarboxylase mRNA is induced anaerobically.

Authors:  P M Kelley
Journal:  Plant Mol Biol       Date:  1989-08       Impact factor: 4.076

7.  Molecular cloning and sequence analysis of an Azospirillum brasilense indole-3-pyruvate decarboxylase gene.

Authors:  A Costacurta; V Keijers; J Vanderleyden
Journal:  Mol Gen Genet       Date:  1994-05-25

8.  Galactokinase encoded by GAL1 is a bifunctional protein required for induction of the GAL genes in Kluyveromyces lactis and is able to suppress the gal3 phenotype in Saccharomyces cerevisiae.

Authors:  J Meyer; A Walker-Jonah; C P Hollenberg
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272

9.  Yeast glycolytic mRNAs are differentially regulated.

Authors:  P A Moore; F A Sagliocco; R M Wood; A J Brown
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272

10.  Induction of pyruvate decarboxylase in glycolysis mutants of Saccharomyces cerevisiae correlates with the concentrations of three-carbon glycolytic metabolites.

Authors:  E Boles; F K Zimmermann
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552
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