| Literature DB >> 31439791 |
Max A English1,2, Luis R Soenksen2,3,4, Raphael V Gayet1,2,5, Helena de Puig2,4, Nicolaas M Angenent-Mari1,2,4, Angelo S Mao2,4,6, Peter Q Nguyen4,6, James J Collins7,2,4,8,9,10.
Abstract
Stimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA-defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black-DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics.Entities:
Mesh：
Substances：
Year: 2019 PMID： 31439791 DOI： 10.1126/science.aaw5122
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728