Literature DB >> 3141570

Construction of a shuttle vector for inducible gene expression in Escherichia coli and Bacillus subtilis.

H Leonhardt1, J C Alonso.   

Abstract

The construction of a shuttle vector for inducible gene expression allowing fast and easy cloning in Escherichia coli and subsequent transformation of Bacillus subtilis is presented. The expression is based on the regulation of the tac promoter by the Lac repressor which was assayed with the xylE gene from Pseudomonas putida as a marker gene. The lacIq gene, transcribed by the strong spo promoter, allowed full repression of the weak tac promoter.

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Mesh：

Year:  1988        PMID： 3141570     DOI： 10.1099/00221287-134-3-605

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  16 in total

1.  Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.

Authors:  David A Schofield; Caroline Westwater; Brian D Hoel; Phillip A Werner; James S Norris; Michael G Schmidt
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

2.  The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.

Authors:  Alexander K W Elsholz; Casper Møller Jørgensen; Robert L Switzer
Journal:  J Bacteriol       Date:  2006-12-08       Impact factor: 3.490

3.  Physical and biochemical characterization of recombination-dependent synthesis of linear plasmid multimers in Bacillus subtilis.

Authors:  H Leonhardt; R Lurz; J C Alonso
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

4.  Nucleotide sequences of Bacillus subtilis flagellar biosynthetic genes fliP and fliQ and identification of a novel flagellar gene, fliZ.

Authors:  D S Bischoff; M D Weinreich; G W Ordal
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

5.  Evidence that the Bacillus subtilis pyrimidine regulatory protein PyrR acts by binding to pyr mRNA at three sites in vivo.

Authors:  Y Lu; R L Switzer
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

6.  Mechanistic diversity in the RuBisCO superfamily: RuBisCO from Rhodospirillum rubrum is not promiscuous for reactions catalyzed by RuBisCO-like proteins.

Authors:  Benjamin P E Warlick; Heidi J Imker; Jaya Sriram; F Robert Tabita; John A Gerlt
Journal:  Biochemistry       Date:  2012-11-14       Impact factor: 3.162

7.  Analysis of structural and biological parameters affecting plasmid deletion formation in Bacillus subtilis.

Authors:  J Kupsch; J C Alonso; T A Trautner
Journal:  Mol Gen Genet       Date:  1989-09

8.  Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription.

Authors:  M T de Souza; M M Lecadet; D Lereclus
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

9.  Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.

Authors:  Qi Meng; Charles L Turnbough; Robert L Switzer
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-13       Impact factor: 11.205

10.  Genetic control of bacterial suicide: regulation of the induction of PBSX in Bacillus subtilis.

Authors:  G E McDonnell; H Wood; K M Devine; D J McConnell
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490
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