Literature DB >> 3134216

Commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence.

B Pozzetto1, O G Gaudin, A Ros, S Tartavel-Mermet.   

Abstract

A commercially available direct immunofluorescence (IF) test using a reagent consisting of a pool of two monoclonal antibodies against selected surface and internal proteins of respiratory syncytial virus (RSV) was evaluated in comparison with the indirect IF technique using a commercial bovine anti-RSV hyperimmune serum for the rapid detection of RSV in nasopharyngeal aspirates from 228 hospitalized children. Overall agreement between the two IF methods was 95%. The direct IF test was quicker to perform and easier to interpret than the indirect IF test.

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Year:  1988        PMID： 3134216     DOI： 10.1007/bf01963083

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  12 in total

1.  Reduction of nonspecific fluorescence in respiratory specimens by pretreatment with N-acetylcysteine.

Authors:  H R Miller; P H Phipps; E Rossier
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

2.  Evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions.

Authors:  S H Cheeseman; L T Pierik; D Leombruno; K E Spinos; K McIntosh
Journal:  J Clin Microbiol       Date:  1986-07       Impact factor: 5.948

3.  Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques.

Authors:  J McQuillin; P S Gardner
Journal:  Br Med J       Date:  1968-03-09

4.  Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay.

Authors:  R K Chao; M Fishaut; J D Schwartzman; K McIntosh
Journal:  J Infect Dis       Date:  1979-04       Impact factor: 5.226

5.  Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.

Authors:  P D Swenson; M H Kaplan
Journal:  J Clin Microbiol       Date:  1986-03       Impact factor: 5.948

6.  Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins.

Authors:  H W Kim; R G Wyatt; B F Fernie; C D Brandt; J O Arrobio; B C Jeffries; R H Parrott
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948

7.  The detection of respiratory syncytial virus in nasopharyngeal aspirates: assessment, formulation, and evaluation of monoclonal antibodies as a diagnostic reagent.

Authors:  A Freke; E J Stott; A P Roome; E O Caul
Journal:  J Med Virol       Date:  1986-02       Impact factor: 2.327

8.  Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.

Authors:  M Grandien; C A Pettersson; P S Gardner; A Linde; A Stanton
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

9.  Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study.

Authors:  C B Hall; J T McBride; E E Walsh; D M Bell; C L Gala; S Hildreth; L G Ten Eyck; W J Hall
Journal:  N Engl J Med       Date:  1983-06-16       Impact factor: 91.245

10.  The development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence.

Authors:  E G Routledge; J McQuillin; A C Samson; G L Toms
Journal:  J Med Virol       Date:  1985-03       Impact factor: 2.327
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  1 in total

1.  Evaluation of four methods for rapid detection of adenovirus.

Authors:  A Trabelsi; B Pozzetto; A D Mbida; F Grattard; A Ros; O G Gaudin
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267
  1 in total

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