Literature DB >> 3020086

Reduction of nonspecific fluorescence in respiratory specimens by pretreatment with N-acetylcysteine.

H R Miller, P H Phipps, E Rossier.   

Abstract

Treatment of nasopharyngeal specimens with 0.25% N-acetylcysteine for 20 min at room temperature effectively reduced the nonspecific fluorescence encountered during direct examination of the specimens by immunofluorescence. The treatment did not affect specific antigen staining or the viability of several common respiratory viruses.

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Year:  1986        PMID： 3020086      PMCID： PMC268940          DOI： 10.1128/jcm.24.3.470-471.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria.

Authors:  G P KUBICA; W E DYE; M L COHN; G MIDDLEBROOK
Journal:  Am Rev Respir Dis       Date:  1963-05

2.  The reduction in vitro in viscosity of mucoprotein solutions by a new mucolytic agent, N-acetyl-L-cysteine.

Authors:  A L SHEFFNER
Journal:  Ann N Y Acad Sci       Date:  1963-03-30       Impact factor: 5.691

3.  Enzymatic lysis of respiratory secretions by aerosol trypsin.

Authors:  C R LIMBER; H G REISER; L C ROETTIG; G M CURTIS
Journal:  J Am Med Assoc       Date:  1952-06-28

4.  Use of dithiothreitol to replace n-acetyl-L-cysteine for routine sputum digestion-decontamination for the culture of mycobacteria.

Authors:  R R Shah; W E Dye
Journal:  Am Rev Respir Dis       Date:  1966-09

5.  Laboratory and clinical evaluation of the mucolytic properties of acetylcysteine.

Authors:  G A Hurst; P B Shaw; C A LeMaistre
Journal:  Am Rev Respir Dis       Date:  1967-11
  5 in total
  7 in total

1.  Respiratory syncytial virus surveillance in various regions across Canada.

Authors: 
Journal:  CMAJ       Date:  1987-01-15       Impact factor: 8.262

2.  Comparison of the VIDAS RSV assay and the Abbott Testpack RSV with direct immunofluorescence for detection of respiratory syncytial virus in nasopharyngeal aspirates.

Authors:  H Miller; R Milk; F Diaz-Mitoma
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

3.  Typing and subtyping of influenza viruses in clinical samples by PCR.

Authors:  K E Wright; G A Wilson; D Novosad; C Dimock; D Tan; J M Weber
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

4.  Evaluation of the QuickLab RSV test, a new rapid lateral-flow immunoassay for detection of respiratory syncytial virus antigen.

Authors:  Robert Slinger; Rose Milk; Isabelle Gaboury; Francisco Diaz-Mitoma
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

5.  Commercial monoclonal antibodies for rapid detection of respiratory syncytial virus by direct immunofluorescence.

Authors:  B Pozzetto; O G Gaudin; A Ros; S Tartavel-Mermet
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-04       Impact factor: 3.267

6.  Respiratory viruses in acute respiratory tract infections in Western India.

Authors:  Leena Ravindra Yeolekar; Rekha Gangadhar Damle; Aarti Nilesh Kamat; Madhukar Ramchandra Khude; Vijay Simha; Anand Neelkanth Pandit
Journal:  Indian J Pediatr       Date:  2008-05-18       Impact factor: 1.967

7.  Comparison of a new commercial enzyme immunoassay for rapid detection of respiratory syncytial virus.

Authors:  M T Garea; J M Lopez; M L Perez del Molino; A Coira; F Pardo
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-02       Impact factor: 3.267
  7 in total

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