Ruili Du1,2,3, Xiandi Zhu1,2,3, Shuang Wu1,2,3, Xiaohui Zhang1,2,3, Yang He4, Kai Zhang1,2,3, Xiao He1,2,3, Xiaoqun Wang5, Yujie Sun6, Qiangbin Wang7, Hong Zhang8,9,10, Mei Tian11,12,13. 1. Department of Nuclear Medicine and PET Center, The Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China. 2. Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, China. 3. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China. 4. Institute of Neuroscience, Zhejiang University School of Medicine, Hangzhou, China. 5. Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. 6. State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), Peking University School of Life Sciences, Beijing, China. 7. Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China. 8. Department of Nuclear Medicine and PET Center, The Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China. hzhang21@zju.edu.cn. 9. Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, China. hzhang21@zju.edu.cn. 10. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China. hzhang21@zju.edu.cn. 11. Department of Nuclear Medicine and PET Center, The Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, China. meitian@zju.edu.cn. 12. Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, China. meitian@zju.edu.cn. 13. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China. meitian@zju.edu.cn.
Abstract
PURPOSE: Stem cell transplantation is promising for temporal lobe epilepsy (TLE) treatment. This study aimed to use PET imaging for the investigation of dynamic metabolic changes after transplantation of human neural stem cells (NSCs) and human GABA progenitor cells (GPCs) in a rat model of TLE. METHODS: 18F-FDG PET imaging, video-electroencephalography (EEG), whole-cell patch-clamp recordings and immunostaining were performed after transplantation of NSCs and GPCs. RESULTS: PET imaging demonstrated that glucose metabolism was gradually improved in the NSCs group, but decreased in GPCs and the control. Video-EEG manifested that seizures were suppressed after NSCs or GPCs transplantation; whole-cell patch-clamp confirmed increased inhibitory response of GPC-derived cells; immunostaining studies verified that the transplanted NSCs and GPCs could survive, migrate and differentiate into mature neuronal subtypes. CONCLUSION: 18F-FDG PET imaging could be a distinguishing approach for evaluation of dynamic glycolytic metabolic changes after transplantation of NSCs and GPCs in TLE. Whole-cell patch-clamp provides evidence for functional maturation and integration of transplanted stem cells within host circuits.
PURPOSE: Stem cell transplantation is promising for temporal lobe epilepsy (TLE) treatment. This study aimed to use PET imaging for the investigation of dynamic metabolic changes after transplantation of human neural stem cells (NSCs) and humanGABA progenitor cells (GPCs) in a rat model of TLE. METHODS:18F-FDG PET imaging, video-electroencephalography (EEG), whole-cell patch-clamp recordings and immunostaining were performed after transplantation of NSCs and GPCs. RESULTS: PET imaging demonstrated that glucose metabolism was gradually improved in the NSCs group, but decreased in GPCs and the control. Video-EEG manifested that seizures were suppressed after NSCs or GPCs transplantation; whole-cell patch-clamp confirmed increased inhibitory response of GPC-derived cells; immunostaining studies verified that the transplanted NSCs and GPCs could survive, migrate and differentiate into mature neuronal subtypes. CONCLUSION:18F-FDG PET imaging could be a distinguishing approach for evaluation of dynamic glycolytic metabolic changes after transplantation of NSCs and GPCs in TLE. Whole-cell patch-clamp provides evidence for functional maturation and integration of transplanted stem cells within host circuits.
Authors: Scott C Baraban; Derek G Southwell; Rosanne C Estrada; Daniel L Jones; Joy Y Sebe; Clara Alfaro-Cervello; Jose M García-Verdugo; John L R Rubenstein; Arturo Alvarez-Buylla Journal: Proc Natl Acad Sci U S A Date: 2009-08-24 Impact factor: 11.205
Authors: Dinesh Upadhya; Bharathi Hattiangady; Olagide W Castro; Bing Shuai; Maheedhar Kodali; Sahithi Attaluri; Adrian Bates; Yi Dong; Su-Chun Zhang; Darwin J Prockop; Ashok K Shetty Journal: Proc Natl Acad Sci U S A Date: 2018-12-17 Impact factor: 11.205