Literature DB >> 3133144

Production of heavy-chain class-switch variants of human monoclonal antibody by recombinant DNA technology.

S Komori1, N Yamasaki, M Shigeta, S Isojima, T Watanabe.   

Abstract

We have previously established a human-mouse heterohybridoma (H6-3C4), which produced a human sperm-immobilizing antibody (mu, lambda of human type). The human rearranged immunoglobulin mu-chain and lambda-chain genes were cloned from the hybridoma H6-3C4. The cloned V region of the heavy chain (VH) gene was ligated to human immunoglobulin gamma 1-heavy chain constant region (C gamma 1) genes. This resulted in the heavy-chain class-switch from mu-chain to gamma 1-chain of H6-3C4 antibody. The class-switched heavy-chain gene as well as the cloned lambda-chain gene were introduced into mouse myeloma cell line X63Ag8.653 by protoplast fusion and electroporation. The stable transformants produced the human IgG monoclonal antibody, which fully retained specificity to human sperm cells and sperm-immobilizing activity.

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Year:  1988        PMID： 3133144      PMCID： PMC1541674     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  22 in total

1.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

2.  A genetically engineered murine/human chimeric antibody retains specificity for human tumor-associated antigen.

Authors:  B G Sahagan; H Dorai; J Saltzgaber-Muller; F Toneguzzo; C A Guindon; S P Lilly; K W McDonald; D V Morrissey; B A Stone; G L Davis
Journal:  J Immunol       Date:  1986-08-01       Impact factor: 5.422

3.  The amino acid sequence of a lambda type Bence-Jones protein. 3. The complete amino acid sequence and the location of the disulfide bridges.

Authors:  K Titani; M Wikler; T Shinoda; F W Putnam
Journal:  J Biol Chem       Date:  1970-04-25       Impact factor: 5.157

4.  Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.

Authors:  J V Ravetch; U Siebenlist; S Korsmeyer; T Waldmann; P Leder
Journal:  Cell       Date:  1981-12       Impact factor: 41.582

5.  A hapten-specific chimaeric IgE antibody with human physiological effector function.

Authors:  M S Neuberger; G T Williams; E B Mitchell; S S Jouhal; J G Flanagan; T H Rabbitts
Journal:  Nature       Date:  1985 Mar 21-27       Impact factor: 49.962

6.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors:  H Potter; L Weir; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

7.  Establishment and characterization of a human hybridoma secreting monoclonal antibody with high titers of sperm immobilizing and agglutinating activities against human seminal plasma.

Authors:  S Isojima; K Kameda; Y Tsuji; M Shigeta; Y Ikeda; K Koyama
Journal:  J Reprod Immunol       Date:  1987-01       Impact factor: 4.054

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

Authors:  R C Mulligan; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

10.  Recombinant human-mouse chimeric monoclonal antibody specific for common acute lymphocytic leukemia antigen.

Authors:  Y Nishimura; M Yokoyama; K Araki; R Ueda; A Kudo; T Watanabe
Journal:  Cancer Res       Date:  1987-02-15       Impact factor: 12.701
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  6 in total

1.  A novel human monoclonal antibody against cervical cancer: its immunoreactivity with normal tube and ovary and with ovarian tumor tissue.

Authors:  T Nakanishi; T Okamoto; A Nawa; T Suzuki; K Ino; Y Wakahara; N Horibe; S Goto; Y Tomoda
Journal:  Arch Gynecol Obstet       Date:  1995       Impact factor: 2.344

2.  Immunoglobulin light chain class multiplicity and alternative organizational forms in early vertebrate phylogeny.

Authors:  J P Rast; M K Anderson; T Ota; R T Litman; M Margittai; M J Shamblott; G W Litman
Journal:  Immunogenetics       Date:  1994       Impact factor: 2.846

3.  Engineering tetravalent IgGs with enhanced agglutination potencies for trapping vigorously motile sperm in mucin matrix.

Authors:  Bhawana Shrestha; Alison Schaefer; Elizabeth C Chavez; Alexander J Kopp; Timothy M Jacobs; Thomas R Moench; Samuel K Lai
Journal:  Acta Biomater       Date:  2020-09-13       Impact factor: 8.947

4.  Engineering sperm-binding IgG antibodies for the development of an effective nonhormonal female contraception.

Authors:  Bhawana Shrestha; Alison Schaefer; Yong Zhu; Jamal Saada; Timothy M Jacobs; Elizabeth C Chavez; Stuart S Olmsted; Carlos A Cruz-Teran; Gabriela Baldeon Vaca; Kathleen Vincent; Thomas R Moench; Samuel K Lai
Journal:  Sci Transl Med       Date:  2021-08-11       Impact factor: 17.956

5.  Hexavalent sperm-binding IgG antibody released from vaginal film for development of potent on-demand nonhormonal female contraception.

Authors:  Bhawana Shrestha; Kathleen Vincent; Alison Schaefer; Yong Zhu; Gracie Vargas; Massoud Motamedi; Kelsi Swope; Josh Morton; Carrie Simpson; Henry Pham; Miles B Brennan; Michael H Pauly; Larry Zeitlin; Barry Bratcher; Kevin J Whaley; Thomas R Moench; Samuel K Lai
Journal:  Proc Natl Acad Sci U S A       Date:  2021-11-30       Impact factor: 11.205

Review 6.  Engineering monoclonal antibody-based contraception and multipurpose prevention technologies†.

Authors:  Deborah J Anderson; Joseph A Politch; Richard A Cone; Larry Zeitlin; Samuel K Lai; Philip J Santangelo; Thomas R Moench; Kevin J Whaley
Journal:  Biol Reprod       Date:  2020-08-04       Impact factor: 4.161
  6 in total

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