Chih-Hung Chiang1,2,3, Ching-Yi Yeh4, Jing Gung Chung5,6, I-Tsang Chiang7,8,9, Fei-Ting Hsu10. 1. Department of Urology, Medical Research and Education, Taipei Veterans General Hospital, Yuan-Shan/Su-Ao Branch, Yilan, Taiwan, R.O.C. 2. Department of Nursing, Cardinal Tien Junior College of Healthcare and Management, New Taipei, Taiwan, R.O.C. 3. Department of Urology, National Taiwan University Hospital, Taipei, Taiwan, R.O.C. 4. Department of Nursing, Taipei Veteran General Hospital, Yuan-Shan/Su-Ao Branch, Yilan, Taiwan, R.O.C. 5. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 6. Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C. 7. Department of Radiation Oncology, Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. sakiro920@gmail.com john740604@gmail.com. 8. Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. 9. Department of Radiation Oncology, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan, R.O.C. 10. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. sakiro920@gmail.com john740604@gmail.com.
Abstract
BACKGROUND/AIM: Amentoflavone has been shown to be effective against a variety of cancer cells, but its role in bladder cancer remains unclear. Thus, the aim of this study is to evaluate whether amentoflavone may induce toxicity effect of bladder cancer. MATERIALS AND METHODS: Herein, we evaluated amentoflavone effects in a human bladder cancer cell line TSGH8301 in vitro. RESULTS: Amentoflavone caused significant cytotoxicity in TSGH8301 cells at a concentration as low as 200 μM. FAS/FASL-dependent extrinsic apoptosis and mitochondria-dependent intrinsic apoptosis were observed in amentoflavone-treated cells in a dose-dependent manner. Levels of several proapoptotic proteins, such as FAS, FAS-ligand and BAX (B-cell lymphoma 2 associated X) were increased following amentoflavone treatment. Meanwhile, anti-apoptotic MCL-1 (myeloid cell leukemia sequence 1) and cellular FLICE-inhibitory protein (C-FLIP) protein levels were reduced. Additionally, angiogenesis and proliferation-related proteins, including matrix metalloproteinase (MMP)-2, -9, vascular endothelial growth factor (VEGF), urokinase-type plasminogen actvator (uPA) and cyclin D1 were diminished by amentoflavone. CONCLUSION: Amentoflavone induced toxicity of bladder cancer by inhibiting tumor progression and inducing apoptosis signaling transduction. Copyright
BACKGROUND/AIM: Amentoflavone has been shown to be effective against a variety of cancer cells, but its role in bladder cancer remains unclear. Thus, the aim of this study is to evaluate whether amentoflavone may induce toxicity effect of bladder cancer. MATERIALS AND METHODS: Herein, we evaluated amentoflavone effects in a humanbladder cancer cell line TSGH8301 in vitro. RESULTS:Amentoflavone caused significant cytotoxicity in TSGH8301 cells at a concentration as low as 200 μM. FAS/FASL-dependent extrinsic apoptosis and mitochondria-dependent intrinsic apoptosis were observed in amentoflavone-treated cells in a dose-dependent manner. Levels of several proapoptotic proteins, such as FAS, FAS-ligand and BAX (B-cell lymphoma 2 associated X) were increased following amentoflavone treatment. Meanwhile, anti-apoptotic MCL-1 (myeloid cell leukemia sequence 1) and cellular FLICE-inhibitory protein (C-FLIP) protein levels were reduced. Additionally, angiogenesis and proliferation-related proteins, including matrix metalloproteinase (MMP)-2, -9, vascular endothelial growth factor (VEGF), urokinase-type plasminogen actvator (uPA) and cyclin D1 were diminished by amentoflavone. CONCLUSION:Amentoflavone induced toxicity of bladder cancer by inhibiting tumor progression and inducing apoptosis signaling transduction. Copyright
Authors: Nico C Grossmann; Victor M Schuettfort; Benjamin Pradere; Marco Moschini; Fahad Quhal; Hadi Mostafaei; Francesco Soria; Satoshi Katayama; Ekaterina Laukhtina; Keiichiro Mori; Reza Sari Motlagh; Cédric Poyet; Mohammad Abufaraj; Pierre I Karakiewicz; Shahrokh F Shariat; David D'Andrea Journal: Onco Targets Ther Date: 2021-01-13 Impact factor: 4.147