Regional Variations of HR-pQCT Morphological and Biomechanical Measurements of the Distal Radius and Tibia and Their Associations with Whole Bone Mechanical Properties.

High-resolution peripheral quantitative computed tomography (HRpQCT) is a promising imaging modality that provides in vivo three-dimensional assessment of bone microstructure by scanning fixed regions of the distal radius and tibia. However, how microstructural parameters and mechanical analysis based on these segment scans correlate to whole distal radius and tibia mechanics is not well-characterized. On 26 sets of cadaveric radius and tibia, HRpQCT scans were performed on the standard scan segment, a segment distal to the standard segment, and a segment proximal to the standard segment. Whole distal bone stiffness was determined through mechanical testing. Segment bone stiffness was estimated using linear finite element (FE) analysis based on segment scans. Standard morphological and Individual Trabecula Segmentation (ITS) analyses were used estimate microstructural properties. Significant variations in microstructural parameters were observed among segments at both sites. Correlation to whole distal bone stiffness was moderate for microstructural parameters at the standard segment, but correlation was significantly increased for FE-predicted segment bone stiffness based on standard segment scans. Similar correlation strengths were found between FE-predicted segment bone stiffness and whole distal bone stiffness. Additionally, microstructural parameters at the distal segment had higher correlation to whole distal bone stiffness than at standard or proximal segments. Our results suggest that FE-predicted segment stiffness is a better predictor of whole distal bone stiffness for clinical HRpQCT analysis, and that microstructural parameters at the distal segment is more highly correlated with whole distal bone stiffness than at the standard or proximal segments.