Mélanie Wlassow1, Lila Poiteau1, Françoise Roudot-Thoraval2, Isabelle Rosa3, Alexandre Soulier1, Christophe Hézode4, Valérie Ortonne1, Jean-Michel Pawlotsky1, Stéphane Chevaliez5. 1. National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology, hôpital Henri Mondor, France; INSERM U955, Créteil, France. 2. Department of Public Health, hôpital Henri Mondor, France; INSERM U955, Créteil, France. 3. Department of Hepatology and Gastroenterology, hôpital Henri Mondor, France. 4. Department of Hepatology and Gastroenterology, Centre Hospitalier Intercommunal de Créteil, France; INSERM U955, Créteil, France. 5. National Reference Center for Viral Hepatitis B, C, and Delta, Department of Virology, hôpital Henri Mondor, France; INSERM U955, Créteil, France. Electronic address: stephane.chevaliez@aphp.fr.
Abstract
BACKGROUND: Sensitive and accurate hepatitis C virus (HCV) RNA detection and quantification are essential to diagnose and monitor the virological response to antiviral treatment and the emergence of resistance. OBJECTIVE AND STUDY DESIGN: The aim of this study was to assess the ability of the new Xpert HCV Viral Load assay to accurately detect and quantify HCV RNA in serum and in whole blood collected on dried blood spot (DBS). Serum and whole blood from a large series of patients chronically infected with different HCV genotypes were tested in parallel for HCV RNA detection and quantification. RESULTS: A significant relationship between HCV RNA levels measured with the Xpert HCV Viral Load assay and the two commercial real-time PCR comparators (Abbott RealTime HCV test and Cobas AmpliPrep/Cobas Taqman HCV 2.0 test) was found in serum as well as in whole blood specimens. CONCLUSIONS: The Xpert HCV Viral Load assay accurately quantifies HCV RNA regardless of the HCV genotype and can thus confidently be used to detect active HCV infection in serum and in whole blood specimens.
BACKGROUND: Sensitive and accurate hepatitis C virus (HCV) RNA detection and quantification are essential to diagnose and monitor the virological response to antiviral treatment and the emergence of resistance. OBJECTIVE AND STUDY DESIGN: The aim of this study was to assess the ability of the new Xpert HCV Viral Load assay to accurately detect and quantify HCV RNA in serum and in whole blood collected on dried blood spot (DBS). Serum and whole blood from a large series of patients chronically infected with different HCV genotypes were tested in parallel for HCV RNA detection and quantification. RESULTS: A significant relationship between HCV RNA levels measured with the Xpert HCV Viral Load assay and the two commercial real-time PCR comparators (Abbott RealTime HCV test and Cobas AmpliPrep/Cobas Taqman HCV 2.0 test) was found in serum as well as in whole blood specimens. CONCLUSIONS: The Xpert HCV Viral Load assay accurately quantifies HCV RNA regardless of the HCV genotype and can thus confidently be used to detect active HCV infection in serum and in whole blood specimens.
Authors: Jason Grebely; Phillip Read; Evan B Cunningham; Martin Weltman; Gail V Matthews; Adrian Dunlop; Mark Montebello; Marianne Martinello; Rosie Gilliver; Philippa Marks; Tanya L Applegate; Gregory J Dore Journal: Health Sci Rep Date: 2020-03-15
Authors: Mehlayl Tariq; Abu Bakar Shoukat; Sedrah Akbar; Samaia Hameed; Muniba Zainab Naqvi; Ayesha Azher; Muhammad Saad; Muhammad Rizwan; Muhammad Nadeem; Anum Javed; Asad Ali; Shahid Aziz Journal: SAGE Open Med Date: 2022-06-29