Isadora Martini Garcia1, Stéfani Becker Rodrigues1, Gabriela de Souza Balbinot1, Fernanda Visioli2, Vicente Castelo Branco Leitune1, Fabrício Mezzomo Collares3. 1. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, Porto Alegre, RS, 90035-003, Brazil. 2. Laboratory of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, Porto Alegre, RS, 90035-003, Brazil. 3. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, Porto Alegre, RS, 90035-003, Brazil. fabricio.collares@ufrgs.br.
Abstract
OBJECTIVES: The aim of this study was to evaluate the influence of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC) in the physico-chemical properties, antibacterial activity and cytotoxicity of an experimental resin-based sealant. MATERIALS AND METHODS: An experimental resin-based sealant was formulated with dimethacrylates and a photoinitiator system. METAC was added at 2.5 wt.% (G2.5%) and 5 wt.% (G5%) into the experimental resin-based sealant, and one group remained without METAC as control (GCTRL). The resin-based sealants were analysed for polymerization behaviour and degree of conversion (DC), Knoop hardness (KHN) and softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), immediate and long-term micro-shear bond strength (μ-SBS) and antibacterial activity and cytotoxicity against human keratinocytes. RESULTS: The experimental resin-based sealants presented different polymerization behaviours without significant differences in the DC (p > 0.05). There was no significant difference for initial KHN (p > 0.05). The ΔKHN ranged from 51.62 (±3.70)% to 62.40 (±4.14)%, with higher values for G5% (p < 0.05). G2.5% and G5% had decreased μ-SBS between immediate and long-term tests (p < 0.05) without significant differences among groups in the immediate and long-term analyses (p > 0.05). There were no significant differences for UTS, contact angle and SFE among groups (p > 0.05). G2.5% and G5% presented immediate and long-term antibacterial activity (p < 0.05) without cytotoxicity compared to GCTRL (p > 0.05). CONCLUSION: The addition of METAC provided antibacterial activity to the experimental resin-based sealant. CLINICAL RELEVANCE: METAC is an effective quaternary ammonium compound as an antibacterial agent for resin-based sealants without cytotoxic effects against human keratinocytes.
OBJECTIVES: The aim of this study was to evaluate the influence of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC) in the physico-chemical properties, antibacterial activity and cytotoxicity of an experimental resin-based sealant. MATERIALS AND METHODS: An experimental resin-based sealant was formulated with dimethacrylates and a photoinitiator system. METAC was added at 2.5 wt.% (G2.5%) and 5 wt.% (G5%) into the experimental resin-based sealant, and one group remained without METAC as control (GCTRL). The resin-based sealants were analysed for polymerization behaviour and degree of conversion (DC), Knoop hardness (KHN) and softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), immediate and long-term micro-shear bond strength (μ-SBS) and antibacterial activity and cytotoxicity against human keratinocytes. RESULTS: The experimental resin-based sealants presented different polymerization behaviours without significant differences in the DC (p > 0.05). There was no significant difference for initial KHN (p > 0.05). The ΔKHN ranged from 51.62 (±3.70)% to 62.40 (±4.14)%, with higher values for G5% (p < 0.05). G2.5% and G5% had decreased μ-SBS between immediate and long-term tests (p < 0.05) without significant differences among groups in the immediate and long-term analyses (p > 0.05). There were no significant differences for UTS, contact angle and SFE among groups (p > 0.05). G2.5% and G5% presented immediate and long-term antibacterial activity (p < 0.05) without cytotoxicity compared to GCTRL (p > 0.05). CONCLUSION: The addition of METAC provided antibacterial activity to the experimental resin-based sealant. CLINICAL RELEVANCE: METAC is an effective quaternary ammonium compound as an antibacterial agent for resin-based sealants without cytotoxic effects against human keratinocytes.
Authors: I G Chestnutt; R Playle; S Hutchings; S Morgan-Trimmer; D Fitzsimmons; N Aawar; L Angel; S Derrick; C Drew; C Hoddell; K Hood; I Humphreys; N Kirby; T M M Lau; C Lisles; M Z Morgan; S Murphy; J Nuttall; K Onishchenko; C Phillips; T Pickles; C Scoble; J Townson; B Withers; B L Chadwick Journal: J Dent Res Date: 2017-04-10 Impact factor: 6.116
Authors: Jaime Aparecido Cury; Branca Heloisa de Oliveira; Ana Paula Pires dos Santos; Livia Maria Andaló Tenuta Journal: Dent Mater Date: 2016-01-06 Impact factor: 5.304
Authors: Abdulrahman A Balhaddad; Mohammed S AlQranei; Maria S Ibrahim; Michael D Weir; Frederico C Martinho; Hockin H K Xu; Mary Anne S Melo Journal: Int J Mol Sci Date: 2020-10-15 Impact factor: 5.923
Authors: Saad Saeed AlShahrani; Mana'a Saleh AlAbbas; Isadora Martini Garcia; Maha Ibrahim AlGhannam; Muath Abdulrahman AlRuwaili; Fabrício Mezzomo Collares; Maria Salem Ibrahim Journal: Materials (Basel) Date: 2021-01-15 Impact factor: 3.623