Isadora Martini Garcia1, Vicente Castelo Branco Leitune2, Fernanda Visioli3, Susana Maria Werner Samuel4, Fabrício Mezzomo Collares5. 1. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: isadora.garcia@ufrgs.br. 2. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: vicente.leitune@ufrgs.br. 3. Laboratory of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: fernanda.visioli@ufrgs.br. 4. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: susana.samuel@ufrgs.br. 5. Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492, Rio Branco, 90035-003, Porto Alegre, RS, Brazil. Electronic address: fabricio.collares@ufrgs.br.
Abstract
OBJECTIVE: To evaluate the influence of zinc oxide quantum dots (ZnOQDs) into an experimental adhesive resin regarding the antibacterial activity against Streptococcus mutans and the cytotoxicity against pulp fibroblasts. MATERIALS AND METHODS: ZnOQDs were synthesized by sol-gel process and were incorporated into 2-hydroxyethyl methacrylate (HEMA). An experimental adhesive resin was formulated by mixing 66.6 wt.% bisphenol A glycol dimethacrylate (BisGMA) and 33.3 wt.% HEMA with a photoinitiator system as control group. HEMA containing ZnOQDs was used for test group formulation. For the antibacterial activity assay, a direct contact inhibition evaluation was performed with biofilm of Streptococcus mutans (NCTC 10449). The cytotoxicity assay was performed by Sulforhodamine B (SRB) colorimetric assay for cell density determination using pulp fibroblasts. Data were analyzed by Student's t-test (α = 0.05). RESULTS: The antibacterial activity assay indicated statistically significant difference between the groups (p = 0.003), with higher values of biofilm formation on the polymerized samples of control group and a reduction of more than 50% of biofilm formation on ZnOQDs group. No difference of pulp fibroblasts viability was found between the adhesives (p = 0.482). CONCLUSION: ZnOQDs provided antibacterial activity when doped into an experimental adhesive resin without cytotoxic effect for pulp fibroblasts. Thus, the use of ZnOQDs is a strategy to develop antibiofilm restorative polymers with non-agglomerated nanofillers. CLINICAL SIGNIFICANCE: ZnOQDs are non-agglomerated nanoscale fillers for dental resins and may be a strategy to reduce biofilm formation at dentin/restoration interface with no cytotoxicity for pulp fibroblasts.
OBJECTIVE: To evaluate the influence of zinc oxide quantum dots (ZnOQDs) into an experimental adhesive resin regarding the antibacterial activity against Streptococcus mutans and the cytotoxicity against pulp fibroblasts. MATERIALS AND METHODS:ZnOQDs were synthesized by sol-gel process and were incorporated into 2-hydroxyethyl methacrylate (HEMA). An experimental adhesive resin was formulated by mixing 66.6 wt.% bisphenol A glycol dimethacrylate (BisGMA) and 33.3 wt.% HEMA with a photoinitiator system as control group. HEMA containing ZnOQDs was used for test group formulation. For the antibacterial activity assay, a direct contact inhibition evaluation was performed with biofilm of Streptococcus mutans (NCTC 10449). The cytotoxicity assay was performed by Sulforhodamine B (SRB) colorimetric assay for cell density determination using pulp fibroblasts. Data were analyzed by Student's t-test (α = 0.05). RESULTS: The antibacterial activity assay indicated statistically significant difference between the groups (p = 0.003), with higher values of biofilm formation on the polymerized samples of control group and a reduction of more than 50% of biofilm formation on ZnOQDs group. No difference of pulp fibroblasts viability was found between the adhesives (p = 0.482). CONCLUSION:ZnOQDs provided antibacterial activity when doped into an experimental adhesive resin without cytotoxic effect for pulp fibroblasts. Thus, the use of ZnOQDs is a strategy to develop antibiofilm restorative polymers with non-agglomerated nanofillers. CLINICAL SIGNIFICANCE: ZnOQDs are non-agglomerated nanoscale fillers for dental resins and may be a strategy to reduce biofilm formation at dentin/restoration interface with no cytotoxicity for pulp fibroblasts.
Authors: Fabrício M Collares; Isadora M Garcia; Mariana Klein; Clarissa F Parolo; Felipe Antonio L Sánchez; Antônio Takimi; Carlos P Bergmann; Susana Maria W Samuel; Mary Anne Melo; Vicente Cb Leitune Journal: Polymers (Basel) Date: 2020-04-02 Impact factor: 4.329
Authors: Z L Shaw; Sruthi Kuriakose; Samuel Cheeseman; Michael D Dickey; Jan Genzer; Andrew J Christofferson; Russell J Crawford; Chris F McConville; James Chapman; Vi Khanh Truong; Aaron Elbourne; Sumeet Walia Journal: Nat Commun Date: 2021-06-23 Impact factor: 14.919
Authors: Louis Hardan; Rim Bourgi; Carlos Enrique Cuevas-Suárez; Maciej Zarow; Naji Kharouf; Davide Mancino; Carlos Fernández Villares; Dariusz Skaba; Monika Lukomska-Szymanska Journal: Microorganisms Date: 2021-06-06