Yanyun Fan1, Ying Shi2,3, Zhenhe Lin1, Xiaoxiao Huang1, Jinying Li2,3, Wei Huang2,3, Dongyan Shen4, Guohong Zhuang5, Wenming Liu6. 1. Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China. 2. Department of Gastroenterology, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, Guangdong Province, China. 3. The First Clinical Medical College, Jinan University, Guangzhou, 510632, Guangdong Province, China. 4. Biobank, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, Fujian Province, China. shendongyan@163.com. 5. Organ Transplantation Institute, Medical College of Xiamen University, Xiamen, 361005, Fujian Province, China. zhgh@xmu.edu.cn. 6. Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China. civilise2008@163.com.
Abstract
BACKGROUND: MicroRNA is essential for the malignant progression of human gastric cancer (GC), which is a leading cause of cancer deaths. However, the mechanism is still not so clear. AIMS: In our present research, we investigated the effect of miR-9-5p in GC. METHODS: We detected miR-9-5p expression in human gastric epithelial cell (GES-1) and GC cells (AGS, BGC-823, MKN-45, and MGC-803), plasma of normal or GC patients, as well as orthotopic xenograft mouse models by real-time PCR. The migration ability was detected by Transwell assays after miR-9-5p mimic or inhibitor transfection in GC cells. RESULTS: Our results showed that miR-9-5p expression in GC cells and plasma was significantly decreased. miR-9-5p inhibited migration of GC cells by regulating TNFAIP8L3 directly. Low expression of miR-9-5p in GC patients hardly suppressed the migration mediated by TNFAIP8L3. CONCLUSIONS: miR-9-5p, as a potential tumor suppressor gene, is closely related to the malignant progression of GC. Exploring the regulation between miR-9-5p and TNFAIP8L3 may provide a novel strategy for GC treatment.
BACKGROUND: MicroRNA is essential for the malignant progression of humangastric cancer (GC), which is a leading cause of cancer deaths. However, the mechanism is still not so clear. AIMS: In our present research, we investigated the effect of miR-9-5p in GC. METHODS: We detected miR-9-5p expression in human gastric epithelial cell (GES-1) and GC cells (AGS, BGC-823, MKN-45, and MGC-803), plasma of normal or GC patients, as well as orthotopic xenograft mouse models by real-time PCR. The migration ability was detected by Transwell assays after miR-9-5p mimic or inhibitor transfection in GC cells. RESULTS: Our results showed that miR-9-5p expression in GC cells and plasma was significantly decreased. miR-9-5p inhibited migration of GC cells by regulating TNFAIP8L3 directly. Low expression of miR-9-5p in GC patients hardly suppressed the migration mediated by TNFAIP8L3. CONCLUSIONS:miR-9-5p, as a potential tumor suppressor gene, is closely related to the malignant progression of GC. Exploring the regulation between miR-9-5p and TNFAIP8L3 may provide a novel strategy for GC treatment.
Authors: Alexandros Laios; Sharon O'Toole; Richard Flavin; Cara Martin; Lynn Kelly; Martina Ring; Stephen P Finn; Ciara Barrett; Massimo Loda; Noreen Gleeson; Tom D'Arcy; Eamonn McGuinness; Orla Sheils; Brian Sheppard; John O' Leary Journal: Mol Cancer Date: 2008-04-28 Impact factor: 27.401
Authors: Qiang Li; Dongmei Yu; Zhengyuan Yu; Qian Gao; Ruifang Chen; Lin Zhou; Rong Wang; Yan Li; Yulan Qian; Jun Zhao; Rafael Rosell; Min Tao; Yufeng Xie; Chun Xu Journal: Transl Lung Cancer Res Date: 2021-02