| Literature DB >> 31133759 |
Maxwell R Mumbach1,2, Jeffrey M Granja1,3, William J Greenleaf4,5,6,7, Howard Y Chang8,9,10,11, Ryan A Flynn1, Caitlin M Roake12,13, Ansuman T Satpathy1,14, Adam J Rubin15, Yanyan Qi1, Zhaozhao Jiang16, Shadi Shams1, Bryan H Louie1, Jimmy K Guo1, David G Gennert1,2, M Ryan Corces1, Paul A Khavari15, Maninjay K Atianand17, Steven E Artandi12, Katherine A Fitzgerald16.
Abstract
Modular domains of long non-coding RNAs can serve as scaffolds to bring distant regions of the linear genome into spatial proximity. Here, we present HiChIRP, a method leveraging bio-orthogonal chemistry and optimized chromosome conformation capture conditions, which enables interrogation of chromatin architecture focused around a specific RNA of interest down to approximately ten copies per cell. HiChIRP of three nuclear RNAs reveals insights into promoter interactions (7SK), telomere biology (telomerase RNA component) and inflammatory gene regulation (lincRNA-EPS).Entities:
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Year: 2019 PMID： 31133759 PMCID： PMC6638558 DOI： 10.1038/s41592-019-0407-x
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547