Assessment of the relationship between serum squamous cell carcinoma antigen (SCC-Ag) concentration in patients with locally advanced squamous cell carcinoma of the uterine cervix and the risk of relapse.

INTRODUCTION: Parameters that will help to identify patients with better and worse prognosis are sought in subjects with locally advanced squamous cell cervical carcinoma. AIM OF THE STUDY: To assess the relationship between squamous cell carcinoma antigen (SCC-Ag) concentration and the risk of relapse in patients with squamous cell cervical carcinoma staged IIB-IIIB.
MATERIAL AND METHODS: The study group consisted of 52 patients with cervical squamous cell carcinoma staged II B (n = 39) and IIIB (n = 13). Serum SCC-Ag concentration was assessed prior to radiochemotherapy or radiotherapy and four weeks after treatment.
RESULTS: The follow-up after treatment ranged from 1 to 33 months (16.2 ±10.5). During follow-up, nine relapses were diagnosed (17.3%). The concentration of SCC-Ag before the treatment was elevated in 41 cases (78.8%) and in 11 cases (21.2%) it was ≤ 2 ng/ml. In all the patients with relapses SCC-Ag concentration before the treatment was elevated. Relapses were diagnosed in five patients with elevated SCC-Ag concentration after the treatment (55.6%) and in four patients with normal SCC-Ag concentration after the treatment (9.3%). There was a positive correlation between SCC-Ag concentration before and after the treatment and relapse occurrence.
CONCLUSIONS: Evaluation of serum SCC-Ag concentration in patients with locally advanced squamous cell cervical carcinoma before treatment is a valuable supplementary diagnostic tool and patients with high SCC-Ag concentration are at an increased risk of relapse. Due to the relationship between elevated serum SCC-Ag concentration after treatment and increased risk of relapse, these patients may require a more intensive post-treatment follow-up.

Introduction

Cervical carcinoma is the third most common malignant neoplasm of the genital organs in women in Poland, after endometrial carcinoma and ovarian carcinoma [1]. Squamous cell carcinoma accounts for about 80% of primary cervical malignancies [2, 3].

The results of treatment in locally advanced cervical carcinoma, i.e. stages IIB-IIIB according to International Federation of Gynecology and Obstetrics (FIGO) [2], depend on a number of factors related to the biology of the tumour and the general condition of patients. Staging, histology and grading are among the parameters characterizing the tumour evaluated in clinical practice. The duration of treatment, number of chemotherapy courses and haemoglobin concentration are also considered as prognostically important [2, 3]. Despite continuous improvement of radiotherapy techniques and progress in systemic treatment, the results of treatment in these patients are still unsatisfactory [3]. Other parameters which will help to identify patients with better and worse prognosis are sought.

The aim of this study was to assess the relationship between the concentration of squamous cell carcinoma antigen (SCC-Ag) and the risk of relapse in patients with locally advanced squamous cell cervical carcinoma.

Material and methods

The study group consisted of 52 patients with cervical squamous cell carcinoma staged IIB (n = 39) and IIIB (n = 13) treated at the Department of Teleradiotherapy, Copernicus Memorial Hospital of Lodz in 2015-2017. Detailed clinical data of the studied population are presented in Table 1. Staging was evaluated clinically based on a pelvic magnetic resonance imaging (MRI) scan. The treatment scheme involved teleradiotherapy to the pelvis, uterus, both adnexa and regional lymph nodes (Fig. 1A, B) up to a dose of 44 Gy fractionated at 2 Gy with weekly injections of cisplatin at a dose of 40 mg/m2. In patients with contraindications to chemotherapy only teleradiotherapy was applied. After teleradiotherapy with or without chemotherapy was completed, high-dose-rate (HDR) brachytherapy was implemented, fractionated at 7 Gy weekly for four weeks up to a total dose of 28 Gy, or teleradiotherapy was continued up to a total dose of 60 Gy. Follow-up appointments after treatment were carried out in the oncological outpatient clinic. Serum SCC-Ag concentration was assessed prior to radiotherapy and four weeks after the end of treatment.

Table 1

Clinical data of studied population

Parameters	Non-relapsed patients		Relapsed patients		Total
	n	%	n	%	n	%
Number of patients	43	82.7	9	17.3	52	100
Menopause
before	8	15.4	2	3.8	10	19.2
after	35	67.3	7	13.5	42	80.8
Childbirth
yes	40	76.9	7	13.5	47	90.4
no	3	5.8	2	3.8	5	9.6
Staging (FIGO)
IIB	36	69.2	3	5.8	39	75.0
IIIB	7	13.5	6	11.5	13	25.0
Histology
G1	7	13.5	0	0	7	13.5
G2	25	48.0	3	5.8	28	53.8
G3	11	21.2	6	11.5	17	32.7
Treatment
RT-CHT	38	73.1	9	17.3	47	90.4
RT	5	9.6	0	0	5	9.6
Median age (years)	60.0 ±10.2		57.4 ±13.2		59.5 ±10.9
Follow-up (months)	16.1 ±10.4		17.7 ±9.3		16.3 ±10.5

Fig. 1

A, B) Irradiation field in teleradiotherapy of cervical carcinoma

The SCC-Ag serum concentration standard for the laboratory where all measurements were performed was 0-2.0 ng/ml. The study was approved by the Bioethics Commission of the Medical University of Lodz no. RN/1/1/15/KE. All data were analysed using Statistica 9.0 software (StatSoft, Tulsa, OK, USA). The p-value lower than 0.05 was considered as significant.

Results

When being diagnosed with primary cancer, the patients were aged between 29 and 78 years (median 59.5 ±10.9); 10 patients (19.2%) were before and 42 patients (80.8%) after the menopause. Forty-seven patients (90.4%) had a history of childbirth. Seven patients (13.5%) had G1 tumours, 28 patients (53.8%) G2 tumours and 17 patients (32.7%) G3 cervical carcinomas. In 47 patients radiochemotherapy was applied and 5 patients received teleradiotherapy up to 44 Gy only. In 50 patients after teleradiotherapy, HDR brachytherapy was used and in 2 patients teleradiotherapy was continued up to a total dose of 60 Gy. The follow-up care after treatment lasted from 1 month to 33 months (median 16.24 ±10.5). During follow-up, 9 relapses (17.3%) were found, including 4 local relapses and 5 distant relapses in the form of metastases to the lungs (n = 2), retroperitoneal lymph nodes (n = 2) and bones (n = 1).

Serum SCC-Ag concentration before the treatment was elevated in 41 cases (78.8%) and in 11 cases (21.2%) it was ≤ 2 ng/ml (p = 0.003). At stage IIB it was 0.6-41.3 ng/ml (median 10.2 ±9.8), and at stage IIIB it was 0.8-89.4 ng/ml (median 36.9 ±11.0; p < 0.001).

In all the patients with relapses serum SCC-Ag concentration before the treatment was elevated and ranged between 4.3 and 89.4 ng/ml (median 23.4 ±14.5), and in 5 of the patients the level was above 10 ng/ml. In 32 patients without relapses (74.4%) the serum SCC-Ag concentration before treatment was elevated, being above 10 ng/ml in 14 cases, whereas in 11 patients (25.6%) it was within normal limits. Serum SCC-Ag concentration before treatment in the non-relapse group was lower than in the relapse group and ranged between 0.7 and 43.8 ng/ml (median 10.0 ±9.1; p < 0.001). In total, among 41 patients with elevated SCC-Ag levels before treatment, 9 (21.9%) were diagnosed with a relapse of cervical carcinoma. None of the patients with a normal SCC-Ag concentration before treatment were diagnosed with a relapse. There was a positive correlation between SCC-Ag concentration before treatment and relapse (r = 0.40; p < 0.05). There was a negative correlation between SCC-Ag concentration before treatment and time to relapse (r = –0.55; p < 0.05).

The frequency of elevated SCC-Ag concentration after treatment was lower than before treatment (p < 0.001; Fig. 2). In all the relapse cases during the follow-up, the concentration of SCC-Ag assessed four weeks after the treatment decreased and ranged between 0.5 and 25.9 ng/ml (median 6.0 ±5.2). In 4 of these cases the concentration of SCC-Ag after the treatment was ≤ 2.0 ng/ml (1 local relapse, 3 distant relapses). In 37 non-relapse cases the concentration of SCC-Ag after the treatment decreased, but it remained above the norm in 4 patients. In the other 6 cases the concentration of SCC-Ag both before and after the treatment was ≤ 2 ng/ml. The concentration of SCC-Ag after the treatment in the non-relapse group was lower than in the relapse group and ranged between 0.4 and 9.2 ng/ml (median 1.2 ±0.9; p < 0.001). A total of 5 out of 9 patients (55.6%) with elevated SCC-Ag concentration after the treatment and 4 out of 43 patients (9.3%) with normal SCC-Ag concentration after the treatment were diagnosed with recurrent cervical carcinoma (p < 0.001). There was a positive correlation between SCC-Ag concentration four weeks after the end of treatment and the occurrence of relapses (r = 0.33; p < 0.05). There was a negative correlation between SCC-Ag concentration after treatment and time to relapse (r = –0.31; p < 0.05).

Fig. 2

Plasma SCC-Ag concentration in patients with locally advanced squamous cell cervical carcinoma before and after treatment

Discussion

This study is valuable because it is prospective, all the SCC-Ag measurements were performed at the same laboratory at predetermined time points related to the treatment, and all the patients were treated according to the same protocol by one medical team. There are no studies in patients with locally advanced squamous cell cervical carcinoma that in prospective observation analyse SCC-Ag concentrations at two time points, i.e. before and after treatment.

Although the relationship between serum levels of SCC-Ag and parameters of cervical carcinoma patients has been the subject of numerous publications, this problem is still unclear. According to Gadducci et al. the concentration of SCC-Ag assessed before treatment is elevated in 28-88% of patients with squamous cell cervical carcinoma and there is a correlation between it and staging, tumour size, depth of stromal infiltration, invasion of lymphatic space and lymph nodes [4]. Similar observations were described by Liu et al. after the analysis of 308 cases in stages IB-IIA, which confirmed that in cases with deep cervical infiltration the concentration of SCC-Ag is elevated [5]. Gaarenstroom, on the other hand, following an analysis of 78 patients, pointed out the relation between elevated SCC-Ag concentration and the parametrial infiltration and the presence of metastases in the lymphatic system [6]. However, not all authors agree with these observations. Salvatici et al., having analysed 197 cases of squamous cell cervical carcinoma in stages I-II, did not confirm the relationship between the concentration of SCC-Ag, staging and grading of squamous cell cervical carcinoma [7]. Bonfrer et al. also reported a lack of correlation between the concentration of SCC-Ag before treatment and staging of cervical carcinoma [8]. Our results in a group of 52 patients with locally advanced squamous cell cervical carcinoma indicate that the serum SCC-Ag concentration at the time of diagnosis is higher in most of these patients and at stage IIIB reaches higher values than in IIB.

Another issue is the possibility of using SCC-Ag concentration assessment in diagnostics of metastases to the lymphatic system. Liu et al. established that the concentration of SCC-Ag is elevated in patients with metastases of cervical carcinoma to the lymphatic system [5]. Analogical views are also presented by other authors [4, 9]. Based on a meta-analysis of 17 studies covering 3985 patients with squamous cell cervical carcinoma, Zhou et al. found that the concentration of SCC-Ag correlates with the presence of metastases to the retroperitoneal lymph nodes and this assay can be used commonly with imaging methods to diagnose metastases to the lymphatic system [9].

The relationship between serum SCC-Ag concentration and cervical carcinoma relapse has also been the subject of numerous studies. In the analysis by Esajas, among 225 cases of early cervical squamous cervical carcinoma, relapses were diagnosed in 35 cases, whereas in 26 cases (74.3%) they were accompanied by elevated levels of SCC-Ag [10]. Similar observations have also been described by other authors [6-8]. Charakorn et al. analysed 61 publications devoted to the relation between SCC-Ag concentration and cervical carcinoma relapse and found that the relative risk of relapse at a high vs. low concentration of SCC-Ag before treatment is 2.44, and when it is assessed after treatment it increases up to 3.91 [11]. In our study, no relapse of cervical carcinoma was observed in any of the patients whose SCC-Ag concentration had not been elevated before the treatment, and the elevated marker concentration four weeks after the end of treatment was associated with an increased risk of relapse. It is believed that if the concentration of SCC-Ag decreases after treatment and then increases again during observation, in 46-92% of cases such an occurrence precedes the clinical diagnosis of a relapse, usually by two to eight months [4]. So far, the relationship between the site of the relapse and the concentration of SCC-Ag has not been confirmed [7].

An increased concentration of SCC-Ag is also associated with an increased risk of death due to cancer. However, it is a less important factor than staging and tumour size [6, 8]. In the study by Esajas, the average survival period after the diagnosis of a relapse was shorter in cases of relapses with a higher SCC-Ag concentration than in cases of relapses without any elevated SCC-Ag concentration (9 vs. 20 months) [10]. We did not study this relationship since the post-treatment observation period was not long enough.

Conclusions

Evaluation of serum SCC-Ag concentration in patients with locally advanced squamous cell cervical carcinoma before treatment is a valuable supplementary diagnostic tool, and patients with high SCC-Ag concentration are at increased risk of relapse.

Due to the relationship between elevated serum SCC-Ag concentration after treatment and increased risk of relapse, these patients may require a more intensive post-treatment follow-up.

Disclosure

The authors report no conflict of interest.