Jia Hu1, Pengpeng Kuang1, Dongni Chen1, Youfang Chen1, Zhesheng Wen1. 1. Department of Thoracic Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
Abstract
BACKGROUND: It is generally believed that the preoperative serum carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCC-Ag) levels are independent predictors of prognosis in multiple malignant tumors. However, their predictive value in esophageal squamous cell carcinoma (ESCC) is still unknown. Therefore, the main purpose of this study is to detect the serum CEA and SCC-Ag levels of ESCC patients before operation, in order to clarify the clinical significance of them as prognostic factors. METHODS: We conducted a retrospective review of 348 patients with ESCC treated by esophagectomy between February 2009 and October 2012. We Analyzed the influence of serum CEA and SCC-Ag level on prognosis. We used a receiver operating characteristic (ROC) curve to identify the serum CEA and SCC-Ag level for predicting survival. We used Log-rank test to compare survival curves, and Cox regression analysis to clarify significant prognostic factors. RESULTS: The cutoffs for CEA and SCC-Ag were 2.28 ng/mL and 0.75 ng/mL, respectively, Under curve area of CEA was 0.600 (95% CI: 0.541-0.660; P=0.001) and under curve area of SCC-Ag was 0.567 (95% CI: 0.507-0.628; P=0.030). According to the Kaplan-Meier curves, the overall survival rate (OS) and disease-free survival rate (DFS) of patients with CEA ≤2.28 ng/mL were higher than those with CEA >2.28 ng/mL. Meanwhile, patients with serum levels of SCC-Ag ≤0.75 ng/mL had a more favorable OS and DFS than those of patients with SCC-Ag >0.75 ng/mL. Cox regression analysis showed that the total mortality of patients with CEA >2.28 ng/mL was higher than that of patients with CEA ≤2.28 ng/mL (HR 1.76; 95% CI: 1.39-2.39; P<0.001). Additionally, SCC-Ag >0.75 ng/mL was an independent negative prognostic factor for DFS (HR 1.86; 95% CI: 1.17-2.96; P=0.009). As the nomogram showed, the survival rate of ESCC patients with high preoperative serum CEA and SCC-Ag levels was relatively low. CONCLUSIONS: High levels of serum CEA and SCC-Ag were independent and significant predictors of ESCC patients after surgical treatment. 2020 Translational Cancer Research. All rights reserved.
BACKGROUND: It is generally believed that the preoperative serum carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCC-Ag) levels are independent predictors of prognosis in multiple malignant tumors. However, their predictive value in esophageal squamous cell carcinoma (ESCC) is still unknown. Therefore, the main purpose of this study is to detect the serum CEA and SCC-Ag levels of ESCC patients before operation, in order to clarify the clinical significance of them as prognostic factors. METHODS: We conducted a retrospective review of 348 patients with ESCC treated by esophagectomy between February 2009 and October 2012. We Analyzed the influence of serum CEA and SCC-Ag level on prognosis. We used a receiver operating characteristic (ROC) curve to identify the serum CEA and SCC-Ag level for predicting survival. We used Log-rank test to compare survival curves, and Cox regression analysis to clarify significant prognostic factors. RESULTS: The cutoffs for CEA and SCC-Ag were 2.28 ng/mL and 0.75 ng/mL, respectively, Under curve area of CEA was 0.600 (95% CI: 0.541-0.660; P=0.001) and under curve area of SCC-Ag was 0.567 (95% CI: 0.507-0.628; P=0.030). According to the Kaplan-Meier curves, the overall survival rate (OS) and disease-free survival rate (DFS) of patients with CEA ≤2.28 ng/mL were higher than those with CEA >2.28 ng/mL. Meanwhile, patients with serum levels of SCC-Ag ≤0.75 ng/mL had a more favorable OS and DFS than those of patients with SCC-Ag >0.75 ng/mL. Cox regression analysis showed that the total mortality of patients with CEA >2.28 ng/mL was higher than that of patients with CEA ≤2.28 ng/mL (HR 1.76; 95% CI: 1.39-2.39; P<0.001). Additionally, SCC-Ag >0.75 ng/mL was an independent negative prognostic factor for DFS (HR 1.86; 95% CI: 1.17-2.96; P=0.009). As the nomogram showed, the survival rate of ESCC patients with high preoperative serum CEA and SCC-Ag levels was relatively low. CONCLUSIONS: High levels of serum CEA and SCC-Ag were independent and significant predictors of ESCC patients after surgical treatment. 2020 Translational Cancer Research. All rights reserved.
Esophageal cancer (EC) is the sixth most common cancer and the fourth leading cause of cancer death in China (1). Epidemiological studies show that the incidence rate of adenocarcinoma is on the rise, and has become the most common subtype of EC in western countries, especially in white patients. On the other hand, squamous cell carcinoma (SCC) comprises approximately 90% of the EC cases in China (2). Esophageal carcinoma has been demonstrated to be one of the most aggressive malignancies and often have a poor prognosis. Although great progress in surgical and oncological treatments have been made, the 5-year survival remains low. Therefore, it is essential to identify reliable prognostic indicators to aid in better treatment recommendations and improve prognosis. The prognostic factors of most gastrointestinal tumors include tumor size, lymph node (LN) metastasis and distant metastasis (3). However, conventional examinations such as computed tomography (CT) or endoscopic ultrasonography have limited role in early detection of microscopic lymph node metastases. Therefore, it is essential to investigate other diagnostic and prognostic markers (4).Serum tumor markers are biological or biochemical substances produced by abnormal tumor cells or stimulated by tumor cells. They are widely used in tumor diagnosis and prognosis prediction. For esophageal squamous cell carcinoma (ESCC) patients, preoperative tumor markers are closely related to tumor invasion depth, lymph node metastasis and prognosis (5,6). Carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag), cytokeratin 19 fragments (Cyfra21-1), and CA19-9 are commonly measured in EC patients. Serum CEA is one of the most widely expressed tumor markers in cancer cells, which is used for preoperative evaluation of colorectal cancer and gastric cancer patients (7,8). Extraction and purification of SCC-Ag from uterine SCC for the first time (9), and SCC-Ag level can predict SCC of cervix and esophagus (10). Cyfra21-1 is a sensitive biomarker in malignant disease, especially in squamous cell carcinoma (11), and CA19-9 is particularly elevated in gastrointestinal cancer (12).CEA and SCC-Ag are common indicators for diagnosis and follow-up of EC, but the sensitivity of biochemical indicators is still not ideal (13,14). Bagaria et al. showed that the combined detection of CEA and CA19-9 was more efficient than single tumor marker in the diagnosis of EC and gastric cancer (8). In this study, the serum CEA and SCC-Ag levels of ESCC patients before operation were measured to clarify its clinical significance as a prognostic factor and its role in guiding treatment.
Methods
Study population, data collection and participant follow-up
From February 2009 to October 2012, 1281 patients with EC underwent therapeutic esophagectomy in Sun Yat-sen University Cancer Center. The exclusion criteria were as follows: (I) patients with tumors of histological subtypes of EC other than ESCC; (II) patients who underwent the sweet approach or for whom the number of removed lymph nodes (RLNs) was less than 15; (III) patients with a history of malignant disease or other primary cancer; (IV) patients with missing data or lost follow-up (exclusion criteria arranged by the investigator). The final study population consisted of 348 patients.The hematological and biochemical profiles of each ESCC patient were evaluated before surgery. Radioimmunoassay (RIA) was used to detect serum CEA and SCC-Ag levels in our clinical laboratory. The normal upper limits of CEA and SCC-Ag were 5.00 and 1.5 ng/mL respectively. The cutoff values of CEA and SCC-Ag were determined by receiver operating characteristic (ROC) curve and Youden index. The cutoff value for CEA was 2.28 ng/mL, while the cutoff value for SCC-Ag was 0.75 ng/mL. According to the TNM classification of the eighth edition of UICC and AJCC, the pathological stage and LN involvement were evaluated (15).After operation, it is recommended to follow up once every 3 months in the first 2 years, once every 6 months in the next 3 years, and once a year later. We observed patients from diagnosis to death or to November 2018, including reviewing the clinical attendance records or telephone interviews with the patient or their family. The average follow-up time was 43.0 months (range, 1–93 months).
Statistical analysis
We used χ2 test to compare the classified variables. The continuous variables were compared by variance (ANOVA). Kaplan Meier method was used to draw survival curve and log rank test was used to evaluate the difference of survival rate between the two groups. Cox Regression Analysis is used in multivariate analysis to determine important prognostic factors. The endpoint of the study was OS. All calculations were carried out using SPSS 17.0 software (SPSS, Chicago, IL) and R (version 3.3.0; http://www.rproject.org). P value less than 0.05 was considered significant.
Results
Patient characteristics and OS
A total of 348 patients were included in the analysis. Preoperative peripheral venous blood of the patients was extracted. CEA and SCC-Ag levels in peripheral venous blood were detected by ELISA. The reagent was purchased from CanAg Company of Sweden. The determination procedure was carried out according to the operation instructions strictly. According to ROC curve and Youden index (Youden index = sensitivity + specificity-1), the cut-off value of CEA was 2.28 ng/mL, and that of SCC-Ag is 0.75 ng/mL (). In addition, the area under the curve of CEA is 0.600 (95% CI: 0.541–0.660; P=0.001; ), and that of SCC-Ag is 0.567 (95% CI: 0.507–0.628; P=0.030; ). According to the critical value, patients were divided into two groups to determine the effect of CEA and SCC-Ag on prognosis.
Figure 1
Receiver operating characteristic were employed to assess the serum levels of CEA and SCC-Ag discriminative performance of the prognosis. (A) The area under the curve (AUC) was 0.600 for CEA (95% CI: 0.541–0.660; P=0.001) and (B) 0.567 for SCC-Ag (95% CI: 0.507–0.628; P=0.030).
Receiver operating characteristic were employed to assess the serum levels of CEA and SCC-Ag discriminative performance of the prognosis. (A) The area under the curve (AUC) was 0.600 for CEA (95% CI: 0.541–0.660; P=0.001) and (B) 0.567 for SCC-Ag (95% CI: 0.507–0.628; P=0.030).The relationship between patient demographics and the cutoff value of CEA and SCC-Ag is summarized in . In general, there were significant differences in TNM stage, gender, surgical complications and prognosis among patients with different CEA levels. Preoperative SCC-Ag level was significantly correlated with T (tumor) stage, preoperative radiotherapy (CRT) and prognosis (P<0.05) (). However, there was no significant correlation between serum CEA, SCC-Ag and other clinical characteristics (P>0.05) (). At the time of this analysis, the median OS was 65.0 months (95% CI: 50.4–79.6 months). In addition, the survival time of patients with high and low serum CEA and SCC-Ag was analyzed and compared. For serum levels of CEA ≤2.28 ng/mL, the median OS was 85.0 months, whereas for patients’ serum levels of CEA >2.28 ng/mL, the median OS was 39.0 months. Similarly, the median OS of patients with serum SCC-Ag ≤0.75 ng/mL was 71.0 months, while that of patients with serum SCC-Ag >0.75 ng/mL was 43.0 months.
Table 1
Baseline characteristics of patients stratified for the cutoff point of the levels of CEA and SCC-Ag
Preoperative serum levels of CEA and SCC-Ag and ESCC patient survival
During the follow-up period, 87 cases recurred in 348 cases and 178 died of all causes. According to Kaplan Meier survival curve, patients with CEA ≤2.28 ng/mL had better OS and DFS than those with CEA >2.28 ng/mL (log rank =16.09, P<0.001; log rank =4.11, P=0.043; , respectively). Meanwhile, patients with serum SCC-Ag ≤0.75 ng/mL had more favorable OS and DFS than those with SCC-Ag >0.75 ng/mL (log rank =5.23, P=0.022; log rank =6.71, P=0.010; ).
Figure 2
Kaplan-Meier analysis of the survival differences classified by the cutoff of CEA. (A) OS in the cohort grouped by the cutoff of CEA (log rank =16.09, P<0.001). (B) Disease free survival in the cohort grouped by the cutoff of CEA (log rank =4.11, P=0.043).
Figure 3
Kaplan-Meier analysis of the survival differences classified by the cutoff of SCC-Ag. (A) OS in the cohort grouped by the cutoff of SCC-Ag (log rank =5.23, P=0.022). (B) Disease free survival in the cohort grouped by the cutoff of SCC-Ag (log rank =6.71, P=0.010).
Kaplan-Meier analysis of the survival differences classified by the cutoff of CEA. (A) OS in the cohort grouped by the cutoff of CEA (log rank =16.09, P<0.001). (B) Disease free survival in the cohort grouped by the cutoff of CEA (log rank =4.11, P=0.043).Kaplan-Meier analysis of the survival differences classified by the cutoff of SCC-Ag. (A) OS in the cohort grouped by the cutoff of SCC-Ag (log rank =5.23, P=0.022). (B) Disease free survival in the cohort grouped by the cutoff of SCC-Ag (log rank =6.71, P=0.010).We conducted Cox regression analysis to assess whether serum CEA and SCC-Ag levels were associated with survival and recurrence after adjustment for potential confounders. Single factor analysis showed that serum CEA, SCC-Ag, N-phase and postoperative CRT levels were significantly correlated with OS (all P<0.05) (). Univariate analysis also showed that gender, the serum level of CEA and SCC-Ag, surgical approaches, N stage, and postoperative CRT were significantly associated with DFS (all P<0.05) (). After adjusting for the confounding covariates, multivariate Cox regression analysis showed that patients with serum levels of CEA >2.28 ng/mL had higher overall mortalities compared to those of patients with serum levels of CEA ≤2.28 ng/mL (HR 1.76; 95% CI: 1.39–2.39; P<0.001). In addition, higher T stage, N metastasis, and complications were independent factors associated with poor survival (). Similarly, multivariate analysis showed that SCC-Ag >0.75 ng/mL was an independent prognostic factor (HR 1.86; 95% CI: 1.17–2.96; P=0.009), while postoperative CRT was an independent prognostic factor (HR 0.60; 95% CI: 0.36-0.82; P<0.001) (). We further investigated the relationship between preoperative serum CEA and SCC-Ag levels and survival in patients with pT3N0M0 ESCC who did not receive adjuvant therapy. Kaplan–Meier analysis demonstrated that pT3N0M0 patients with a serum level of CEA >2.28 ng/mL had a poor outcome compared to that in patients with CEA ≤2.28 ng/mL (log rank =11.56, P=0.001; ), while the relationship between pT3N0M0 patient prognosis and the serum level of SCC-Ag was not statistically significant (log rank =2.92, P=0.087; ).
Table S1
Univariate Cox regression analysis of prognostic factors influencing OS and DFS
Kaplan-Meier analysis of the survival differences classified by the cutoff of CEA and SCC-Ag in pT3N0M0 patients. (A) OS in pT3N0M0 patients grouped by the cutoff of CEA (log rank =11.56, P=0.001). (B) Disease free survival in pT3N0M0 patients grouped by the cutoff of SCC-Ag (log rank =2.92, P=0.087).
HR, hazard ratio; CI, confidence interval; CEA, carcinoembryonic antigen; SCC-Ag, squamous cell carcinoma antigen; CRT, chemoradiation therapy.Kaplan-Meier analysis of the survival differences classified by the cutoff of CEA and SCC-Ag in pT3N0M0 patients. (A) OS in pT3N0M0 patients grouped by the cutoff of CEA (log rank =11.56, P=0.001). (B) Disease free survival in pT3N0M0 patients grouped by the cutoff of SCC-Ag (log rank =2.92, P=0.087).The nomogram constructed for predicting the prognosis is depicted in . The model consisted of the serum level of CEA and SCC-Ag, T stage and N stage. The predicted C index of Harrell survival rate was 0.70. The calibration curves showed a good agreement between the probability of the survival rate by a nomogram and the ideal probability (). As shown in the model, the survival rate of ESCC patients with high preoperative serum CEA and SCC levels may be relatively low.
Figure 5
The nomogram consisted of the serum level of CEA and SCC-Ag, T stage and N stage. The nomogram was composed of 9 rows. Row 1 (“Points”) is the point assignment for each corresponding variable in the model (Row 2 to 5), which is determined by drawing a vertical line from the appropriate position on the variable row to the points row. Then the total is marked in row 6 (“Total points”) when the assigned points for all 4 variables are added. Finally, survival possibility will be presented by drawing a vertical line from the appropriate position on the row Total Points to the corresponding row. Levels above the cut-off values of CEA and SCC-Ag were defined as “1”, while those below the value were defined as “2”. CEA, carcinoembryonic antigen; SCC-Ag, squamous cell carcinoma antigen.
Figure S1
Calibration curves of the nomogram in predicting patient survival at (A) 1-year, (B) 2-year and (C) 3-year.
The nomogram consisted of the serum level of CEA and SCC-Ag, T stage and N stage. The nomogram was composed of 9 rows. Row 1 (“Points”) is the point assignment for each corresponding variable in the model (Row 2 to 5), which is determined by drawing a vertical line from the appropriate position on the variable row to the points row. Then the total is marked in row 6 (“Total points”) when the assigned points for all 4 variables are added. Finally, survival possibility will be presented by drawing a vertical line from the appropriate position on the row Total Points to the corresponding row. Levels above the cut-off values of CEA and SCC-Ag were defined as “1”, while those below the value were defined as “2”. CEA, carcinoembryonic antigen; SCC-Ag, squamous cell carcinoma antigen.
Discussion
The aim of this research was to investigate the relationship between CEA, SCC-Ag and the prognosis of ESCC. A ROC curve was applied to verify the cutoff point of CEA and SCC-Ag for OS prediction Our study identified values of 2.28 ng/mL as the optimum cutoff point for CEA while 0.75 ng/mL as the optimum cutoff point for SCC-Ag, in predicting prognosis in patients with ESCC.CEA is one of the most widely used and easily obtained tumor markers in gastrointestinal cancer, especially colorectal cancer (7,8,16,17). In addition, detecting the level of serum tumor markers is a routine preoperative examination for ESCC patients. Due to the relatively low sensitivity and specificity of CEA and SCC-Ag to ESCC, the specific function of these tumor markers in ESCC patients is not clear clinically. Our research showed that serum CEA >2.28 ng/mL was an independent risk factor for poor OS, and SCC-Ag >0.75 ng/mL was an independent negative prognostic factor for DFS. Lee et al. reported that patients with CEA levels >10 ng/mL had higher rates of lymph node involvement and deeper tumor infiltration than other patients (7). Mihmanli et al. found that the elevation of CEA level was related to the depth of invasion and pathological stage (16). In addition, Munck-Wikland et al. reported that the increase of CEA level is related to the distant metastasis of EC, which may reflect the metastasis potential of EC cells (17). Therefore, an elevated level of preoperative CEA served as a poor prognostic marker for ESCC patients after surgical resection. In addition, it should be noted that regular postoperative monitoring of CEA may help to identify patients with metastatic diseases after surgical treatment. A previous study reported that CEA had a higher positivity rate for ESCC than that of cytokeratin-18 and CA19-9, and the authors believe that CEA level is helpful for the detection of postoperative recurrence of gastric cancer and EC and the evaluation of therapeutic response (18).CEA is an acidic glycoprotein found in thin films of colon and rectal cancer and in embryonic mucosa cells. It can be widely found in the digestive system cancer of endodermal origin, in the digestive tract tissue of normal embryo, and in the serum of normal human. SCC-Ag is an important tumor marker of squamous cell carcinoma. It is separated from cervical squamous cell carcinoma and exists in the cytoplasm of squamous cell carcinoma. When the cells undergo malignant transformation and tumor growth, the synthesis or expression of these antigens is significantly increased due to the de inhibition of the corresponding coding genes. Secondly, genes that were not expressed were reactivated during tumorigenesis. Thirdly, abnormal glycosylation results in special degradation products of protein. What’s more, some aspects of antigen synthesis are abnormal, such as labnormal ectopic expression of embryo antigen or differentiation antigen. In addition, overexpression of gene products, especially signaling molecules leads to tumor antigen elevation (19,20). Based on these factors, serum CEA and SCC-Ag were increased in ESCC patients. Because CEA and SCC-Ag are considered to reflect systemic inflammatory response (SIR) on the basis of hypercytokinemia caused by tumor host interaction, the prognosis of CEA and SCC-Ag may be significantly different (21).Previous studies have reported that elevated CEA and SCC-Ag levels are independent predictors of poor OS in EC patients, and the best cutoff time is uncertain (6,22,23). Ma et al. found that serum SCC-Ag, CEA, CA19-9 were significantly correlated with lymph node metastasis. According to the normal upper limit at their institute, they stated that the cutoff for CEA was 5 µg/L while the cutoff for SCC-Ag was 1.5 µg/L (24). Similarly, Oki et al. defined the cut-off value of CEA as 5 ng/mL because it is the upper limit of normal level (22). Cao et al. suggested that high preoperative levels of CYFRA21-1 and SCC-Ag have a negative impact on the survival of stage II ESCC patients, and those authors also defined the cut off as the normal upper limit of 1.5 µg/L for SCC-Ag (6). Zhang et al. reported that CEA level is an independent predictor of the prognosis of ESCC patients treated with CRT at the same time. According to 95% CIs of cancer-free patients in China, the critical value of CEA is 3.3 ng/mL (23). Huang et al. constructed a prognostic model that combined C-reactive protein and CEA, which proved to be a more precise prognostic factor in ESCC patients. These authors concluded that the optimum cutoff point for CEA was 4.2 ng/mL for predicting cancer- specific survival (CSS) as verified by ROC curves, and the AUC was 0.618 for CEA (25). In clinical practice, we found that ESCC patients with early and medial stages of the disease usually present with serum levels of CEA and SCC-Ag in the normal range. Therefore, according to ROC analysis, the critical values of CEA and SCC-Ag are 2.28 and 0.75 ng/mL, respectively, which are lower than previous studies. We reasoned that we did not defined the cutoff as the normal upper limit, as most of the patients included in our study were in the early and medial stages of the disease and were primarily eligible for surgical treatment, which justifies the lower cutoff values.The relationship between serum SCC-Ag concentration and patient survival was also discussed earlier (5,26,27). Mroczko et al. found that SCC-Ag and CEA had no significant effect on the prognosis of ESCC (27). However, Kosugi et al. found that a high level of serum SCC-Ag before esophagectomy predicted a negative prognosis in patients after esophagectomy. They found a significant correlation between SCC-Ag and tumor invasion depth, lymph node status, TNM stage and vascular invasion (5). Shimada et al. obtained similar results, in which high preoperative SCC-Ag concentration is an important prognostic factor (28). SCC-Ag production has been proven to be enhanced by epidermal growth factor in cervical cancer cells, suggesting that high SCC levels may be involved in elevated tumor proliferative ability (29). Additionally, as an inhibitory serpin, SCC-Ag may function as a regulator of the differentiation of normal squamous epithelium or as an inhibitor of apoptosis in cancer tissue (30).Considering that there are controversies about whether pT3N0M0 ESCC patients should undergo adjuvant treatment after surgery (31), we further analyzed the association between the serum levels of CEA and prognosis for pT3N0M0 ESCC patients who did not receive adjuvant therapy. We found that pT3N0M0 patients with preoperative serum level of CEA >2.28 ng/mL had a poor prognosis compared to that patients with lower levels of CEA. Therefore, we recommended that pT3N0M0 patients with CEA >2.28 ng/mL preoperatively should receive adjuvant treatment even after radical esophagectomy. We further constructed a nomogram using clinical parameters of T stage, N stage and preoperative serum CEA and SCC-Ag levels. The values “0” and “1” represent values below and above the cutoff, respectively. We evaluate an acceptable calibration curve by looking at the nomograph prediction probability versus actual probability (), and a concordance index (c-index) of 0.70 was obtained. These results suggested that our nomogram has an acceptable predictive accuracy (31). This nomogram may contribute to clinical decision-making in terms of postoperative treatment. According to the nomogram, higher serum levels of CEA have a more prominent effect on patient outcome than SCC-Ag, and ESCC patients with high serum levels of both CEA and SCC may suffer a relatively low survival rate. For instance, a patient with pT3N0M0 staging and levels of both CEA and SCC-Ag that are higher than the cutoff (value =1) would score 6.8 total points and have a 5-year survival rate of only approximately 50% (). Adjuvant treatment would be recommended for patients with this profile to improve survival even if lymph node involvement was not detected.There were 53 patients who received neoadjuvant CRT in the cohort. Antitumor therapy may have an effect on the concentration of related tumor markers, only the serum CEA and SCC-Ag levels after treatment are included in the analysis. We found that neoadjuvant CRT could lower the serum levels of tumor markers to some extent, especially for CEA. However, the results were not presented in detail because of the limited size. We reasoned that neoadjuvant therapy plus surgery could serve as a protective factor for OS in EC patients (32), which may be related to the reduced tumor markers.Inaccurate LN dissection and pathological evaluation may result in inappropriate pathologic nodal staging and treatment, a phenomenon called stage migration (33). In order to reduce the possibility of non-standard lymphadenectomy leading to incorrect pathological staging, our study only included patients who received the Ivor Lewis approach (44 cases) and the three-incision approach (304 cases). According to NCCN guidelines for the treatment of EC (in 2016), at least 15 lymph nodes should be removed during radical resection of EC (34). In the current study, patients with RLNs <15 were excluded to ensure accurate pN staging and radical resection. This is the first study to adopt these inclusion criteria and discuss the relationship between tumor marker level and prognosis.The strength of the current study is that we studied ESCC patients from a single institution using relatively standard surgical techniques to ensure accurate pathological staging and the radical resection. The representative sample and the considerably high follow-up rate guarantee the reliability of our results. Nevertheless, there are several limitations that should be considered. First, this is a single center retrospective analysis, which requires a larger cohort of prospective studies to confirm our results. Second, we excluded patients of other histological types. Therefore, caution is needed in extrapolating our findings to these patients. In addition, we included patients who received primary resection and preoperative CRT. However, there were few subjects in the preoperative CRT group. A subgroup analysis was not performed.
Conclusions
In conclusion, we demonstrate that high levels of preoperative serum CEA and SCC-Ag are independent and significant predictors of postoperative ESCC. Higher serum levels of CEA had a more prominent effect on patient outcome than SCC-Ag, and ESCC patients with high serum levels of both CEA and SCC may suffer a relatively low survival rate, which may help clinicians to decide on the adjuvant treatment after surgery. Future prospective studies with larger populations are needed to validate our findings and to explore the potential mechanisms underlying the prognostic value of tumor markers.
Authors: Marta Lukaszewicz-Zając; Barbara Mroczko; Mirosław Kozłowski; Jacek Nikliński; Jerzy Laudański; Maria Siewko; Maciej Szmitkowski Journal: J Clin Lab Anal Date: 2012-02 Impact factor: 2.352
Authors: Luis Vence; A Karolina Palucka; Joseph W Fay; Tomoki Ito; Yong-Jun Liu; Jacques Banchereau; Hideki Ueno Journal: Proc Natl Acad Sci U S A Date: 2007-12-18 Impact factor: 11.205
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Figure S1