Farahnaz Moghadamnia1, Pegah Ghoraeian1, Sara Minaeian2, Atefeh Talebi3, Farnaz Farsi4, Abolfazl Akbari5,6. 1. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran. 2. Institute of Immunology and Infectious Diseases, Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran. 3. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran. 4. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. 5. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran. akbari.ab@iums.ac.ir. 6. Colorectal Research Center, Rasoul-e- Akram Hospital, Sattarkhan Ave, Niyayesh St, Tehran, Iran. akbari.ab@iums.ac.ir.
Abstract
INTRODUCTION: MicroRNAs (miRNAs), as a family of non-coding RNAs, have opened a new window in cancer biology and transcriptome. It has been revealed that miRNAs post-transcriptionally regulate the gene expression and involve in colorectal cancer (CRC) development and progression. Our aim was to examine the differential expression of miRNAs in a CRC and to correlate their expression levels with mRNA levels of CRC-related genes (K-ras, APC, p53). MATERIALS AND METHODS: Seventy-two colorectal tumor tissues from patients with newly diagnosed CRC and 72 matched normal adjacent tissues were analyzed. Relative expression of seven CRC-related miRNAs (miR-21, miR-31, miR-20a, miR-133b, and miR-145, miR-135b and let-7g) and three CRC-related genes (K-ras, APC, p53) was detected using the SYBR Green quantitative real-time PCR technique. The correlation between gene expression levels and clinicopathological features was evaluated. RESULTS: Our results showed a significant difference between the two groups for the expression level of miR-21, miR-31, miR-145, and miR-20a (P < 0.001). Also, a significant difference between the two groups for the expression level of K-ras was found (P < 0.001). Further analysis revealed an inverse significant correlation between miR-145 and K-ras (R2 = 0.662, P < 0.001), while a positive correlation was observed between miR-21 and K-ras (R2 = 0.732, P < 0.001). CONCLUSION: Dysregulation of miRNAs and correlation with molecular signaling pathways designated a biological role for miRNAs in various cellular mechanisms underlying CRC. On the other hand, the pattern of miRNAs expression and its correlation with transcriptional status are helpful to discovery biomarkers and design therapeutics for CRC.
INTRODUCTION: MicroRNAs (miRNAs), as a family of non-coding RNAs, have opened a new window in cancer biology and transcriptome. It has been revealed that miRNAs post-transcriptionally regulate the gene expression and involve in colorectal cancer (CRC) development and progression. Our aim was to examine the differential expression of miRNAs in a CRC and to correlate their expression levels with mRNA levels of CRC-related genes (K-ras, APC, p53). MATERIALS AND METHODS: Seventy-two colorectal tumor tissues from patients with newly diagnosed CRC and 72 matched normal adjacent tissues were analyzed. Relative expression of seven CRC-related miRNAs (miR-21, miR-31, miR-20a, miR-133b, and miR-145, miR-135b and let-7g) and three CRC-related genes (K-ras, APC, p53) was detected using the SYBR Green quantitative real-time PCR technique. The correlation between gene expression levels and clinicopathological features was evaluated. RESULTS: Our results showed a significant difference between the two groups for the expression level of miR-21, miR-31, miR-145, and miR-20a (P < 0.001). Also, a significant difference between the two groups for the expression level of K-ras was found (P < 0.001). Further analysis revealed an inverse significant correlation between miR-145 and K-ras (R2 = 0.662, P < 0.001), while a positive correlation was observed between miR-21 and K-ras (R2 = 0.732, P < 0.001). CONCLUSION: Dysregulation of miRNAs and correlation with molecular signaling pathways designated a biological role for miRNAs in various cellular mechanisms underlying CRC. On the other hand, the pattern of miRNAs expression and its correlation with transcriptional status are helpful to discovery biomarkers and design therapeutics for CRC.
Authors: Laura Cantini; Michele Caselle; Antoine Forget; Andrei Zinovyev; Emmanuel Barillot; Loredana Martignetti Journal: Front Biosci (Landmark Ed) Date: 2017-06-01
Authors: Ioannis S Vlachos; Konstantinos Zagganas; Maria D Paraskevopoulou; Georgios Georgakilas; Dimitra Karagkouni; Thanasis Vergoulis; Theodore Dalamagas; Artemis G Hatzigeorgiou Journal: Nucleic Acids Res Date: 2015-05-14 Impact factor: 16.971